MS-Fit Search Results


.

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): HSG-100
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 88 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/62(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.429e+0059 (14)27.014.5-54.430.1 35077/7.6HUMANGuanine nucleotide-binding protein beta subunit-like protein 12.3 (P205) (Receptor of activated protein kinase C 1) (RACK1) (Receptor for activated C kinase)
29204 (6)5.06.5-34.883.9 99400/5.2HUMANLipin 2
36295 (8)6.08.1-26.368.3 69873/7.8HUMANTyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
44164 (6)9.06.5-35.154.3 38178/6.5HUMANLeucine-rich alpha-2-glycoprotein precursor (LRG)
52825 (8)8.08.1-28.664.2 69900/6.3HUMANBaculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 2) (HIAP2) (HIAP-2) (C-IAP1) (TNFR2-TRAF signaling complex protein 2) (IAP homolog B)
81538................x......x...............x...x..x..x..xx.x......
105062................x....................................xx......x
40982........x....x.x...................................x.....x....
53605.....x.......x.x.......................................x......
18491....xx.........................x..............x...x...........


Detailed Results

1. 9/62 matches (14%).
Acc. #: Species: HUMAN Name: Guanine nucleotide-binding protein beta subunit-like protein 12.3 (P205) (Receptor of activated protein kinase C 1) (RACK1) (Receptor for activated C kinase)
Index: MW: 35077 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1018.46001018.5144-531761830(K) (L)
1059.53681059.5951-553093170(R) (-)
1192.50241192.5598-4848570(R) (A)
1309.54901309.6388-691071180(K) (Q)
1562.73711562.7927-3645571(K) (A)
1788.86901788.9959-712652801(K) (A)
1978.87281978.9987-641011181(R) (Q)
1995.75411995.8871-671401550(K) (F)
2036.97992037.0365-281071251(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (87/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/62 matches (6%).
Acc. #: Species: HUMAN Name: Lipin 2
Index: MW: 99400 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1018.46001018.5243-637887950(K) (N)
1995.75411995.8547-502582740(R) (V)
2011.90502011.8496281Met-ox 2582740(R)SESHMEWTWGGFPESTK(V)
2383.84122383.9683-531691910(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 5% (48/896AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/62 matches (8%).
Acc. #: Species: HUMAN Name: Tyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
Index: MW: 69873 Da pI: 7.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
982.3606982.3974-373783850(K) (E)
998.3726998.3923-201Met-ox 3783850(K)ADTEEMMR(E)
1014.41761014.3872302Met-ox 3783850(K)ADTEEMMR(E)
1977.86021977.9605-515805941(K) (A)
2210.99732211.1153-534314500(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 6% (43/619AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/62 matches (6%).
Acc. #: Species: HUMAN Name: Leucine-rich alpha-2-glycoprotein precursor (LRG)
Index: MW: 38178 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
968.5105968.5205-102402470(R) (L)
998.3726998.4366-643213280(K) (C)
1014.41761014.4315-141Met-ox 3213280(K)MFSQNDTR(C)
2036.97992037.0868-521922090(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (34/347AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/62 matches (8%).
Acc. #: Species: HUMAN Name: Baculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 2) (HIAP2) (HIAP-2) (C-IAP1) (TNFR2-TRAF signaling complex protein 2) (IAP homolog B)
Index: MW: 69900 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
960.3644960.3845-212342410(K) (R)
968.5105968.4941175305370(K) (N)
1126.49691126.5753-701Met-ox 3333401(R)CEFLIRMK(G)
1562.73711562.7749-242592720(R) (M)
1940.80311940.8911-4520360(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (55/618AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.