MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-11
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 259 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.015e+00820 (33)40.033.3-11.526.8 74140/6.6HUMANLamin A/C (70 kDa lamin)
21.018e+00616 (26)29.026.7-13.028.0 74210/6.5MOUSELamin A
33.206e+00515 (25)31.025.0-13.528.7 65447/6.4MOUSELamins C and C2
42.870e+00510 (16)23.016.7-6.3037.4 72334/5.1HUMAN78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BIP) (Endoplasmic reticulum lumenal Ca2+ binding protein grp78)
53.360e+0049 (15)20.015.0-5.9139.6 72423/5.1MOUSE78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BIP)
81589....xx...xx..xx.x.xxx..xx..x.xxx.x....x................xx...
15157....xx...xx..xx...xx...x...x.xxxxx....x.....................
118002....xx...xx..xx...xx...x.....xxxxx....x.....................
13425......................x.xxxx.......x.x..x...x...x...........
109822......................x.xxxx.......x.x..x...x...............


Detailed Results

1. 20/60 matches (33%).
Acc. #: Species: HUMAN Name: Lamin A/C (70 kDa lamin)
Index: MW: 74140 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1023.48051023.5111-302092160(K) (E)
1028.54761028.5740-262412490(R) (A)
1089.53691089.5540-1651600(R) (L)
1148.55561148.5799-2163720(R) (E)
1182.57981182.6119-271571660(R) (G)
1187.61921187.584329pyroGlu 1Met-ox 1821901(K)QLQDEMLRR(V)
1187.61921187.6384-163203291(K) (E)
1274.58041274.6017-17pyroGlu 4724820(R)QNGDDPLLTYR(F)
1347.65461347.6731-141Met-ox 3673770(K)LALDMEIHAYR(K)
1359.64921359.6868-2812250(R) (I)
1377.61831377.6334-115165270(K) (T)
1475.74581475.7681-151Met-ox 3673781(K)LALDMEIHAYRK(L)
1525.73101525.7572-171Met-ox 1972081(R)LQTMKEELDFQK(N)
1566.74201566.7512-5.96286440(R) (S)
1605.79021605.8124-144404531(R) (L)
1629.80571629.8084-1.629411(R) (L)
1699.95501699.9707-9.22973111(R) (Q)
1752.85251752.8629-6.02812960(R) (I)
1909.91641909.9217-2.81Met-ox 3523660(R)MQQQLDEYQELLDIK(L)
2365.16312365.15951.55986240(K) (S)
2533.33072533.32034.14915150(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (269/664AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/60 matches (26%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1023.48051023.5111-302092160(K) (E)
1028.54761028.5740-262412490(R) (A)
1089.53691089.5540-1651600(R) (L)
1148.55561148.5799-2163720(R) (E)
1182.57981182.6119-271571660(R) (G)
1187.61921187.584329pyroGlu 1Met-ox 1821901(K)QLQDEMLRR(V)
1187.61921187.6384-163203291(K) (E)
1347.65461347.6731-141Met-ox 3673770(K)LALDMEIHAYR(K)
1359.64921359.6868-2812250(R) (I)
1475.74581475.7681-151Met-ox 3673781(K)LALDMEIHAYRK(L)
1566.74201566.7512-5.96296450(R) (S)
1605.79021605.8124-144404531(R) (L)
1629.80571629.8084-1.629411(R) (L)
1699.95501699.9707-9.22973111(R) (Q)
1707.77141707.8050-204214351(K) (T)
1752.85251752.8629-6.02812960(R) (I)
1909.91641909.9217-2.81Met-ox 3523660(R)MQQQLDEYQELLDIK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (197/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 15/60 matches (25%).
Acc. #: Species: MOUSE Name: Lamins C and C2
Index: MW: 65447 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1023.48051023.5111-302092160(K) (E)
1028.54761028.5740-262412490(R) (A)
1089.53691089.5540-1651600(R) (L)
1148.55561148.5799-2163720(R) (E)
1182.57981182.6119-271571660(R) (G)
1187.61921187.584329pyroGlu 1Met-ox 1821901(K)QLQDEMLRR(V)
1187.61921187.6384-163203291(K) (E)
1347.65461347.6731-141Met-ox 3673770(K)LALDMEIHAYR(K)
1359.64921359.6868-2812250(R) (I)
1475.74581475.7681-151Met-ox 3673781(K)LALDMEIHAYRK(L)
1605.79021605.8124-144404531(R) (L)
1629.80571629.8084-1.629411(R) (L)
1699.95501699.9707-9.22973111(R) (Q)
1707.77141707.8050-204214351(K) (T)
1752.85251752.8629-6.02812960(R) (I)
1909.91641909.9217-2.81Met-ox 3523660(R)MQQQLDEYQELLDIK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (180/574AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: 78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BIP) (Endoplasmic reticulum lumenal Ca2+ binding protein grp78)
Index: MW: 72334 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1430.72021430.6916201021130(R) (F)
1525.73101525.6844305415531(R) (K)
1528.72601528.7470-141Met-ox 3253361(R)AKFEELNMDLFR(S)
1552.74841552.7933-291Met-ox 1391520(K)TFAPEEISAMVLTK(M)
1566.74201566.7804-2461740(R) (L)
1815.97891815.9969-9.91982141(R) (E)
1887.95401887.9717-9.41651810(K) (Q)
1934.01431934.01360.384754920(K) (G)
1999.05121999.0864-184935100(R) (V)
2164.97142164.9926-9.83073240(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (153/654AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/60 matches (15%).
Acc. #: Species: MOUSE Name: 78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BIP)
Index: MW: 72423 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1430.72021430.6916201031140(R) (F)
1525.73101525.6844305425541(R) (K)
1528.72601528.7470-141Met-ox 3263371(R)AKFEELNMDLFR(S)
1552.74841552.7933-291Met-ox 1401530(K)TFAPEEISAMVLTK(M)
1566.74201566.7804-2462750(R) (L)
1815.97891815.9969-9.91992151(R) (E)
1887.95401887.9717-9.41661820(K) (Q)
1934.01431934.01360.384764930(K) (G)
1999.05121999.0864-184945110(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (135/655AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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