MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-45
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 312 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.972e+006	11 (18)	31.0	18.3	-8.28	46.5		46268/8.3		HUMAN	47 kDa heat shock protein precursor (Collagen-binding protein 1) (Colligin 1)
2	1.965e+006	13 (21)	37.0	21.7	-9.47	42.9		46441/8.7		HUMAN	Collagen-binding protein 2 precursor (Colligin 2) (Rheumatoid arthritis related antigen RA-A47)
3	5921	8 (13)	13.0	13.3	15.1	32.3		96493/5.4		MOUSE	Heat-shock protein 105 kDa (Heat shock-related 100 kDa protein E7I) (HSP-E7I) (Heat shock 110 kDa protein) (42 degrees C-HSP)
4	2629	6 (10)	20.0	10.0	-0.374	71.3		36926/9.0		MOUSE	Beta-1,3-N-acetylglucosaminyltransferase radical fringe
5	2425	7 (11)	19.0	11.7	-6.09	35.1		46590/8.9		MOUSE	47 kDa heat shock protein precursor (Collagen-binding protein 1) (Serine protease inhibitor J6)

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48410	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 11/60 matches (18%).
Acc. #: Species: HUMAN Name: 47 kDa heat shock protein precursor (Collagen-binding protein 1) (Colligin 1)
Index: MW: 46268 Da pI: 8.3

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
986.5143	986.5635	-50		94	102	0	(K) (D)
1224.6274	1224.6588	-26		308	318	0	(K) (H)
1293.6584	1293.6803	-17		393	404	0	(R) (L)
1337.7038	1337.7429	-29		319	331	0	(K) (N)
1437.7093	1437.7338	-17		103	115	0	(R) (S)
1579.8669	1579.8808	-8.8		319	333	1	(K) (A)
1637.7685	1637.7335	21		239	251	1	(R) (L)
1659.7910	1659.8019	-6.6		133	147	0	(R) (S)
2000.1086	2000.1003	4.1	1Met-ox	263	279	0	(K)LSSLIILMPHHVEPLER(L)
2405.2949	2405.2789	6.7		94	115	1	(K) (S)
2551.5132	2551.4347	31		60	85	0	(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (131/417AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/60 matches (21%).
Acc. #: Species: HUMAN Name: Collagen-binding protein 2 precursor (Colligin 2) (Rheumatoid arthritis related antigen RA-A47)
Index: MW: 46441 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
986.5143 986.5635 -50 95 103 0 (K) (D)
1224.6274 1224.6588 -26 309 319 0 (K) (H)
1293.6584 1293.6803 -17 394 405 0 (R) (L)
1337.7038 1337.7429 -29 320 332 0 (K) (N)
1359.5617 1359.5859 -18 3Met-ox 229 239 0 (R)SYTVGVMMMHR(T)
1437.7093 1437.7338 -17 104 116 0 (R) (S)
1473.7201 1473.7412 -14 1Met-ox 47 60 0 (R)SAGLAFSLYQAMAK(D)
1579.8669 1579.8808 -8.8 320 334 1 (K) (A)
1637.7685 1637.7335 21 240 252 1 (R) (L)
1659.7910 1659.8019 -6.6 134 148 0 (R) (S)
2000.1086 2000.1003 4.1 1Met-ox 264 280 0 (K)LSSLIILMPHHVEPLER(L)
2405.2949 2405.2789 6.7 95 116 1 (K) (S)
2551.5132 2551.4347 31 61 86 0 (K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (156/418AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/60 matches (13%).
Acc. #: Species: MOUSE Name: Heat-shock protein 105 kDa (Heat shock-related 100 kDa protein E7I) (HSP-E7I) (Heat shock 110 kDa protein) (42 degrees C-HSP)
Index: MW: 96493 Da pI: 5.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1293.6584 1293.6302 22 240 249 0 (K) (T)
1296.6835 1296.6548 22 186 196 0 (K) (V)
1308.6409 1308.6510 -7.7 85 95 0 (K) (N)
1425.7291 1425.6936 25 610 621 0 (R) (M)
1637.7685 1637.7117 35 738 750 1 (K) (K)
1940.9581 1940.9718 -7.0 357 374 1 (K) (G)
2267.0983 2267.0371 27 1Met-ox 755 773 1 (K)SVNEVMEWMNNVMNAQAKR(S)
2420.2980 2420.2859 5.0 792 812 1 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (115/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Beta-1,3-N-acetylglucosaminyltransferase radical fringe
Index: MW: 36926 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
986.5143 986.5536 -40 82 89 1 (R) (Q)
1179.5691 1179.5614 6.5 110 119 0 (R) (T)
1659.7910 1659.7545 22 122 134 1 (R) (F)
1675.7631 1675.7494 8.2 1Met-ox 122 134 1 (R)QALCCKMSVEYDK(F)
1940.9581 1941.0459 -45 253 268 0 (R) (L)
2211.1412 2211.0392 46 90 109 0 (R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (67/332AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/60 matches (11%).
Acc. #: Species: MOUSE Name: 47 kDa heat shock protein precursor (Collagen-binding protein 1) (Serine protease inhibitor J6)
Index: MW: 46590 Da pI: 8.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1224.6274 1224.6588 -26 308 318 0 (K) (H)
1296.6835 1296.6808 2.0 252 262 0 (K) (L)
1337.7038 1337.7429 -29 319 331 0 (K) (N)
1579.8669 1579.8808 -8.8 319 333 1 (K) (A)
1637.7685 1637.7335 21 239 251 1 (R) (L)
1659.7910 1659.8019 -6.6 133 147 0 (R) (S)
2000.1086 2000.1003 4.1 1Met-ox 263 279 0 (K)LSSLIILMPHHVEPLER(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (82/417AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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