MS-Fit Search Results


.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-56
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 413 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
17.654e+01016 (26)50.026.7-29.426.6 47169/7.0HUMANAlpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
22.391e+0079 (15)34.015.0-28.731.7 47141/6.4MOUSEAlpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1)
31.497e+00511 (18)18.018.3-1.6867.1 69873/7.8HUMANTyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
46.791e+0047 (11)18.011.7-23.159.3 49478/5.8HUMANAlpha enolase, lung specific (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (HLE1)
56.172e+0046 (10)19.010.0-35.215.3 46987/7.6HUMANBeta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (MSE) (Enolase 3)
65205.xx.......xx..x.......xx...........xxx...x...x..x......xx.x.
41608.xx........x..........xx.................x...x..x......x....
42773.......xx..x...........x...x....x..x........x....x.x...x....
27268.x........x...x...x...x.x................x..................
27136...........x..x...x...xx...............................x....


Detailed Results

1. 16/60 matches (26%).
Acc. #: Species: HUMAN Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
Index: MW: 47169 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1143.56031143.6162-491841930(R) (N)
1406.66551406.7167-3616280(R) (G)
1540.70951540.7834-482402530(K) (S)
1541.71161541.7647-341Met-ox 3593720(K)LAQANGWGVMVSHR(S)
1556.72031556.7783-371Met-ox 2402530(K)VVIGMDVAASEFFR(S)
1647.76391647.8376-453443580(K) (L)
1804.88521804.9445-3333500(R) (D)
1907.92571907.9876-321631790(K) (E)
1923.91211923.9825-371Met-ox 1631790(K)LAMQEFMILPVGAANFR(E)
1939.92331939.9774-282Met-ox 1631790(K)LAMQEFMILPVGAANFR(E)
1960.88591960.9252-202032210(K) (E)
2033.00812033.0555-233073260(K) (R)
2192.04292192.0698-121Met-ox 2342531(K)AGYTDKVVIGMDVAASEFFR(S)
2367.12292367.1754-223733940(R) (T)
2510.09932510.1152-6.32863060(K) (F)
3011.54793011.5703-7.41331620(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 50% (219/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/60 matches (15%).
Acc. #: Species: MOUSE Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1)
Index: MW: 47141 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1143.56031143.6162-491841930(R) (N)
1406.66551406.7167-3616280(R) (G)
1541.71161541.7647-343593720(K) (S)
1647.76391647.8376-453443580(K) (L)
1804.88521804.9445-3333500(R) (D)
1960.88591960.9252-202032210(K) (E)
2033.00812033.0555-233073260(K) (R)
2192.04292192.03344.42342530(K) (S)
2367.12292367.1754-223733940(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (151/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/60 matches (18%).
Acc. #: Species: HUMAN Name: Tyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
Index: MW: 69873 Da pI: 7.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1508.70771508.636147pyroGlu 3733851(K)QGTEKADTEEMMR(E)
1525.67821525.6627103733851(K) (E)
1541.71161541.6576351Met-ox 3733851(K)QGTEKADTEEMMR(E)
1804.88521804.88301.22832981(R) (I)
1826.88661826.9223-203864000(R) (L)
1891.92951891.8761281611751(R) (K)
1907.92571907.8710291Met-ox 1611751(R)MPWYHSSLTREEAER(K)
1993.87051993.9554-431Met-ox 5805941(K)WEDRPDFLTVEQRMR(A)
2211.05002211.1153-304314500(R) (Y)
2233.05512233.1399-383343531(K) (Q)
2367.12292367.2165-404304501(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (115/619AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Alpha enolase, lung specific (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (HLE1)
Index: MW: 49478 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1143.56031143.6162-491932020(R) (N)
1540.70951540.6556352662780(K) (Y)
1556.72031556.7783-372492620(K) (D)
1572.71071572.7732-401Met-ox 2492620(K)VVIGMDVAASEFYR(D)
1647.76391647.8376-453653790(K) (L)
1809.82501809.8363-6.2961111(K) (F)
1960.88591960.9252-202122300(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (87/458AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Beta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (MSE) (Enolase 3)
Index: MW: 46987 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1541.71161541.7647-343593720(K) (S)
1556.72031556.7783-372402530(K) (N)
1572.71071572.7732-401Met-ox 2402530(K)VVIGMDVAASEFYR(N)
1647.76391647.8376-453443580(K) (L)
1804.88521804.9445-3333500(R) (D)
2367.12292367.1754-223733940(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (83/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.