MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-66
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 700 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/76(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	2.549e+007	17 (22)	52.0	22.4	16.1	47.2	42040/8.5	HUMAN	3-ketoacyl-CoA thiolase, mitochondrial (Beta-ketothiolase) (Acetyl-CoA acyltransferase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1)
2	3.664e+006	14 (18)	36.0	18.4	4.51	40.4	44730/7.6	MOUSE	Putative GTP-binding protein PTD004 homolog
3	3.663e+006	14 (18)	36.0	18.4	4.51	40.4	44744/7.6	HUMAN	Putative GTP-binding protein PTD004 (PRO2455)
4	6.348e+004	7 (9)	22.0	9.2	7.70	38.3	44537/7.5	MOUSE	Phosphoglycerate kinase 1
5	1.212e+004	6 (7)	10.0	7.9	-10.2	46.1	56558/5.7	HUMAN	Sestrin 1 (p53-regulated protein PA26)

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112987	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 17/76 matches (22%).
Acc. #: Species: HUMAN Name: 3-ketoacyl-CoA thiolase, mitochondrial (Beta-ketothiolase) (Acetyl-CoA acyltransferase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1)
Index: MW: 42040 Da pI: 8.5

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
955.4784	955.5114	-35		271	278	0	(K) (I)
993.5217	993.5118	10.0		182	189	0	(K) (W)
1083.5830	1083.6063	-22		270	278	1	(K) (I)
1123.5993	1123.6152	-14		15	25	0	(R) (D)
1188.6770	1188.6853	-7.0		361	370	0	(R) (R)
1279.7240	1279.7163	6.0		14	25	1	(K) (D)
1415.6812	1415.6695	8.3		26	38	0	(K) (A)
1668.8127	1668.7652	28		177	189	1	(R) (W)
1835.0082	1834.9524	30		341	360	0	(K) (I)
1954.9226	1954.8857	19		192	209	0	(K) (T)
1970.9300	1970.8806	25	1Met-ox	192	209	0	(K)AANDAGYFNDEMAPIEVK(T)
2325.1964	2325.1073	38	1Met-ox	313	332	0	(K)DMDLVEVNEAFAPQYLAVER(S)
2380.2657	2380.1567	46	pyroGlu 1Met-ox	215	234	0	(K)QTMQVDEHARPQTTLEQLQK(L)
2387.1339	2387.0681	28	1Met-ox	110	130	0	(K)EAEVVLCGGTESMSQAPYCVR(N)
2397.2770	2397.1832	39	1Met-ox	215	234	0	(K)QTMQVDEHARPQTTLEQLQK(L)
2516.3341	2516.2480	34		242	269	0	(K) (K)
2767.4877	2767.3824	38	1Met-ox	46	71	0	(K)VSPETVDSVIMGNVLQSSSDAIYLAR(H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 52% (210/397AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/76 matches (18%).
Acc. #: Species: MOUSE Name: Putative GTP-binding protein PTD004 homolog
Index: MW: 44730 Da pI: 7.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
959.4599 959.4951 -37 387 394 0 (K) (K)
1074.5074 1074.5431 -33 271 279 0 (K) (Q)
1276.7292 1276.7377 -6.7 6 18 0 (K) (F)
1404.8414 1404.8327 6.2 5 18 1 (K) (F)
1481.7253 1481.7310 -3.8 1Met-ox 282 294 0 (K)YLEANMTQSALPK(I)
1568.9652 1568.9416 15 84 98 0 (K) (G)
1572.8344 1572.7950 25 374 386 0 (R) (F)
1654.8426 1654.8052 23 71 83 0 (R) (I)
1707.8131 1707.8126 0.27 341 354 1 (K) (D)
1835.9955 1835.9941 0.79 1Met-ox 282 297 1 (K)YLEANMTQSALPKIIK(A)
1910.0944 1910.0864 4.2 6 24 1 (K) (I)
1949.9955 1949.9398 29 202 216 1 (R) (H)
2014.9740 2014.9358 19 126 143 0 (R) (D)
2025.0525 2025.0082 22 pyroGlu 370 386 1 (R)QQGRNYIVEDGDIIFFK(F)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
959.4599	959.4951	-37		387	394	0	(K) (K)
1074.5074	1074.5431	-33		271	279	0	(K) (Q)
1276.7292	1276.7377	-6.7		6	18	0	(K) (F)
1404.8414	1404.8327	6.2		5	18	1	(K) (F)
1481.7253	1481.7310	-3.8	1Met-ox	282	294	0	(K)YLEANMTQSALPK(I)
1568.9652	1568.9416	15		84	98	0	(K) (G)
1572.8344	1572.7950	25		374	386	0	(R) (F)
1654.8426	1654.8052	23		71	83	0	(R) (I)
1707.8131	1707.8126	0.27		341	354	1	(K) (D)
1835.9955	1835.9941	0.79	1Met-ox	282	297	1	(K)YLEANMTQSALPKIIK(A)
1910.0944	1910.0864	4.2		6	24	1	(K) (I)
1949.9955	1949.9398	29		202	216	1	(R) (H)
2014.9740	2014.9358	19		126	143	0	(R) (D)
2025.0525	2025.0082	22	pyroGlu	370	386	1	(R)QQGRNYIVEDGDIIFFK(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (145/396AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 14/76 matches (18%).
Acc. #: Species: HUMAN Name: Putative GTP-binding protein PTD004 (PRO2455)
Index: MW: 44744 Da pI: 7.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
959.4599 959.4951 -37 387 394 0 (K) (K)
1074.5074 1074.5431 -33 271 279 0 (K) (Q)
1276.7292 1276.7377 -6.7 6 18 0 (K) (F)
1404.8414 1404.8327 6.2 5 18 1 (K) (F)
1481.7253 1481.7310 -3.8 1Met-ox 282 294 0 (K)YLEANMTQSALPK(I)
1568.9652 1568.9416 15 84 98 0 (K) (G)
1572.8344 1572.7950 25 374 386 0 (R) (F)
1654.8426 1654.8052 23 71 83 0 (R) (I)
1707.8131 1707.8126 0.27 341 354 1 (K) (E)
1835.9955 1835.9941 0.79 1Met-ox 282 297 1 (K)YLEANMTQSALPKIIK(A)
1910.0944 1910.0864 4.2 6 24 1 (K) (I)
1949.9955 1949.9398 29 202 216 1 (R) (H)
2014.9740 2014.9358 19 126 143 0 (R) (D)
2025.0525 2025.0082 22 pyroGlu 370 386 1 (R)QQGRNYIVEDGDIIFFK(F)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
959.4599	959.4951	-37		387	394	0	(K) (K)
1074.5074	1074.5431	-33		271	279	0	(K) (Q)
1276.7292	1276.7377	-6.7		6	18	0	(K) (F)
1404.8414	1404.8327	6.2		5	18	1	(K) (F)
1481.7253	1481.7310	-3.8	1Met-ox	282	294	0	(K)YLEANMTQSALPK(I)
1568.9652	1568.9416	15		84	98	0	(K) (G)
1572.8344	1572.7950	25		374	386	0	(R) (F)
1654.8426	1654.8052	23		71	83	0	(R) (I)
1707.8131	1707.8126	0.27		341	354	1	(K) (E)
1835.9955	1835.9941	0.79	1Met-ox	282	297	1	(K)YLEANMTQSALPKIIK(A)
1910.0944	1910.0864	4.2		6	24	1	(K) (I)
1949.9955	1949.9398	29		202	216	1	(R) (H)
2014.9740	2014.9358	19		126	143	0	(R) (D)
2025.0525	2025.0082	22	pyroGlu	370	386	1	(R)QQGRNYIVEDGDIIFFK(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (145/396AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/76 matches (9%).
Acc. #: Species: MOUSE Name: Phosphoglycerate kinase 1
Index: MW: 44537 Da pI: 7.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1083.5830 1083.6050 -20 407 417 0 (K) (-)
1614.8748 1614.8889 -8.7 217 230 1 (K) (V)
1634.7865 1634.7927 -3.8 157 171 0 (K) (A)
1769.0256 1768.9961 17 200 216 0 (K) (V)
1982.9953 1982.9381 29 247 264 0 (K) (I)
1998.9919 1998.9330 29 1Met-ox 247 264 0 (K)VLNNMEIGTSLYDEEGAK(I)
2023.0625 2023.0388 12 280 297 1 (K) (T)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1083.5830	1083.6050	-20		407	417	0	(K) (-)
1614.8748	1614.8889	-8.7		217	230	1	(K) (V)
1634.7865	1634.7927	-3.8		157	171	0	(K) (A)
1769.0256	1768.9961	17		200	216	0	(K) (V)
1982.9953	1982.9381	29		247	264	0	(K) (I)
1998.9919	1998.9330	29	1Met-ox	247	264	0	(K)VLNNMEIGTSLYDEEGAK(I)
2023.0625	2023.0388	12		280	297	1	(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (93/417AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/76 matches (7%).
Acc. #: Species: HUMAN Name: Sestrin 1 (p53-regulated protein PA26)
Index: MW: 56558 Da pI: 5.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1074.5074 1074.5067 0.59 31 39 0 (R) (I)
1276.7292 1276.7741 -35 465 475 0 (K) (M)
1648.8525 1648.9209 -41 476 489 1 (R) (Y)
1954.9226 1954.9308 -4.2 379 394 0 (K) (D)
1970.9300 1970.9257 2.2 1Met-ox 379 394 0 (K)FHIAYNLTYNTMAMHK(D)
1986.9548 1986.9206 17 2Met-ox 379 394 0 (K)FHIAYNLTYNTMAMHK(D)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1074.5074	1074.5067	0.59		31	39	0	(R) (I)
1276.7292	1276.7741	-35		465	475	0	(K) (M)
1648.8525	1648.9209	-41		476	489	1	(R) (Y)
1954.9226	1954.9308	-4.2		379	394	0	(K) (D)
1970.9300	1970.9257	2.2	1Met-ox	379	394	0	(K)FHIAYNLTYNTMAMHK(D)
1986.9548	1986.9206	17	2Met-ox	379	394	0	(K)FHIAYNLTYNTMAMHK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (50/492AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):