MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-83
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 579 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/67(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.716e+007	13 (19)	40.0	19.4	-40.4	30.6		35993/8.7		MOUSE	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
2	1.650e+007	13 (19)	38.0	19.4	-40.4	30.6		37430/9.0		HUMAN	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
3	2.693e+004	6 (8)	16.0	9.0	-21.7	53.1		71289/6.0		MOUSE	GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
4	6840	6 (8)	13.0	9.0	-12.7	70.8		66268/5.3		MOUSE	DNA POLYMERASE ALPHA 70 KDA SUBUNIT (DNA POLYMERASE SUBUNIT B)
5	6490	9 (13)	35.0	13.4	-34.5	18.6		36054/8.6		HUMAN	Glyceraldehyde 3-phosphate dehydrogenase, liver

81777	x	x	x	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.
119594	x	x	x	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.
110305	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.
85413	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.
117116	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.

Detailed Results

1. 13/67 matches (19%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 35993 Da pI: 8.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1013.3804	1013.4441	-63		192	201	0	(R) (G)
1050.3829	1050.4420	-56		118	125	0	(R) (I)
1087.4225	1087.4849	-57		27	34	0	(R) (L)
1188.5814	1188.6476	-56		126	135	0	(K) (Q)
1377.5682	1377.6300	-45		202	216	0	(R) (G)
1410.6233	1410.6878	-46		162	173	0	(K) (K)
1695.7021	1695.7655	-37		142	156	0	(R) (I)
1798.8516	1798.9227	-40		11	26	0	(K) (N)
1851.8352	1851.8666	-17		141	156	1	(K) (I)
1879.8991	1879.9666	-36		102	117	1	(K) (D)
1926.9539	1927.0177	-33		10	26	1	(R) (N)
2205.8595	2205.9008	-19	1Met-ox	314	338	0	(R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.0242	2220.0712	-21		118	135	1	(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (137/341AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/67 matches (19%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 37430 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1013.3804 1013.4441 -63 204 213 0 (R) (G)
1050.3829 1050.4420 -56 130 137 0 (R) (I)
1087.4225 1087.4849 -57 39 46 0 (R) (L)
1188.5814 1188.6476 -56 138 147 0 (K) (Q)
1377.5682 1377.6300 -45 214 228 0 (R) (G)
1410.6233 1410.6878 -46 174 185 0 (K) (K)
1695.7021 1695.7655 -37 154 168 0 (R) (I)
1798.8516 1798.9227 -40 23 38 0 (K) (N)
1851.8352 1851.8666 -17 153 168 1 (K) (I)
1879.8991 1879.9666 -36 114 129 1 (K) (D)
1926.9539 1927.0177 -33 22 38 1 (R) (N)
2205.8595 2205.9008 -19 1Met-ox 326 350 0 (R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.0242 2220.0712 -21 130 147 1 (R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (137/353AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/67 matches (8%).
Acc. #: Species: MOUSE Name: GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
Index: MW: 71289 Da pI: 6.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1614.8295 1614.8280 0.91 341 354 0 (R) (A)
1628.8325 1628.8284 2.5 478 492 0 (K) (N)
1838.8376 1838.9149 -42 59 74 1 (R) (E)
1851.8352 1851.8289 3.4 462 477 0 (R) (T)
2036.0153 2036.1028 -43 516 537 0 (R) (E)
2211.0177 2211.1331 -52 564 584 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (104/643AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/67 matches (8%).
Acc. #: Species: MOUSE Name: DNA POLYMERASE ALPHA 70 KDA SUBUNIT (DNA POLYMERASE SUBUNIT B)
Index: MW: 66268 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1838.8376 1838.9037 -36 168 185 0 (R) (G)
1848.8484 1848.9206 -39 370 385 0 (R) (H)
2041.0365 2040.9258 54 pyroGlu 36 54 0 (R)QTEDGMVSELIAFCTSAGK(T)
2245.0593 2245.0963 -16 293 312 0 (K) (R)
2401.1147 2401.1974 -34 293 313 1 (K) (L)
2611.3001 2611.3116 -4.4 168 194 1 (R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (83/600AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/67 matches (13%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1233.5539 1233.5972 -35 2Met-ox 324 334 0 (R)VVDLMAHMASK(E)
1544.7450 1544.8107 -43 235 248 0 (R) (L)
1613.8374 1613.9015 -40 67 80 0 (K) (D)
1763.7393 1763.8029 -36 310 323 0 (K) (V)
1861.8648 1861.9516 -47 146 162 0 (K) (V)
2041.0365 2041.1082 -35 67 84 1 (K) (I)
2245.0593 2245.0997 -18 2Met-ox 119 139 0 (R)VIISAPSADAPMFVMGVNHEK(Y)
2401.1147 2401.2008 -36 2Met-ox 118 139 1 (K)RVIISAPSADAPMFVMGVNHEK(Y)
2611.3001 2611.3554 -21 1Met-ox 163 186 0 (K)VIHDNFGIVEGLMTTVHAITATQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (120/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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