MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-87
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 162 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/59(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
19.328e+00716 (27)56.027.1-40.316.6 34196/9.3MOUSEHeterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1) (HDP-1) (Topoisomerase-inhibitor suppressed)
28.211e+00716 (27)48.027.1-40.316.6 38846/9.3HUMANHeterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1)
350596 (10)14.010.2-14.263.9 82274/9.2MOUSEZinc finger protein 60 (Zfp-60) (Zinc finger protein Mfg-3)
432825 (8)9.08.54.7258.5 75492/6.0HUMANCoagulation factor XIII B chain precursor (Protein-glutamine gamma-glutamyltransferase B chain) (Transglutaminase B chain) (Fibrin stabilizing factor B subunit)
530955 (8)5.08.5-30.429.0 95487/7.9MOUSEUbiquitin carboxyl-terminal hydrolase 26 (Ubiquitin thiolesterase 26) (Ubiquitin-specific processing protease 26) (Deubiquitinating enzyme 26)
111679x.xx.........x..x..x.....xxx..xx..x....x.........x.x..x....
109746x.xx.........x..x..x.....xxx..xx..x....x.........x.x..x....
47670.............xx..................xxx.......x...............
89901..x....................x...........x........x......x.......
27805....x...........................x............x.x.x.........


Detailed Results

1. 16/59 matches (27%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1) (HDP-1) (Topoisomerase-inhibitor suppressed)
Index: MW: 34196 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1049.40761049.4580-481231300(R) (I)
1234.57871234.6353-461Met-ox 1311400(K)IEVIEIMTDR(G)
1299.61211299.6545-334141(K) (K)
1437.67871437.7450-46931050(R) (K)
1499.64061499.6814-271671780(K) (K)
1628.72161628.7821-372853000(R) (N)
1694.63601694.7006-383013180(R) (R)
1699.67711699.7604-491471610(R) (I)
1784.83671784.9071-3916310(K) (S)
1855.77791855.8615-451461611(K) (I)
1879.88981879.9666-411071221(K) (D)
1912.91611913.0020-4515311(R) (S)
1995.86981995.9057-181Met-ox 32470(R)SHFEQWGTLTDCVVMR(D)
2195.86582195.9634-441141301(K) (I)
2281.04842281.1505-451471661(R) (Y)
2526.09992526.2087-431Met-ox 56780(R)GFGFVTYATVEEVDAAMNARPHK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 56% (181/320AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/59 matches (27%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1)
Index: MW: 38846 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1049.40761049.4580-481231300(R) (I)
1234.57871234.6353-461Met-ox 1311400(K)IEVIEIMTDR(G)
1299.61211299.6545-334141(K) (K)
1437.67871437.7450-46931050(R) (K)
1499.64061499.6814-271671780(K) (K)
1628.72161628.7821-373373520(R) (N)
1694.63601694.7006-383533700(R) (R)
1699.67711699.7604-491471610(R) (I)
1784.83671784.9071-3916310(K) (S)
1855.77791855.8615-451461611(K) (I)
1879.88981879.9666-411071221(K) (D)
1912.91611913.0020-4515311(R) (S)
1995.86981995.9057-181Met-ox 32470(R)SHFEQWGTLTDCVVMR(D)
2195.86582195.9634-441141301(K) (I)
2281.04842281.1505-451471661(R) (Y)
2526.09992526.2087-431Met-ox 56780(R)GFGFVTYATVEEVDAAMNARPHK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (181/372AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/59 matches (10%).
Acc. #: Species: MOUSE Name: Zinc finger protein 60 (Zfp-60) (Zinc finger protein Mfg-3)
Index: MW: 82274 Da pI: 9.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1437.67871437.6473224774870(R) (E)
1439.63231439.6854-373093190(K) (V)
1893.88401893.9558-381191370(K) (E)
1893.88401893.9758-486416551(R) (E)
1912.91611912.8468361170(-) (D)
1917.87361917.9030-154885031(K) (T)
2058.83022058.8690-196786931(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (105/707AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/59 matches (8%).
Acc. #: Species: HUMAN Name: Coagulation factor XIII B chain precursor (Protein-glutamine gamma-glutamyltransferase B chain) (Transglutaminase B chain) (Fibrin stabilizing factor B subunit)
Index: MW: 75492 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1234.57871234.5818-2.544530(K) (K)
1681.68141681.7532-431761900(K) (K)
1917.87361917.8475143833970(K) (H)
2103.02292102.9639283813971(R) (H)
2281.04842280.9865271291470(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (61/661AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/59 matches (8%).
Acc. #: Species: MOUSE Name: Ubiquitin carboxyl-terminal hydrolase 26 (Ubiquitin thiolesterase 26) (Ubiquitin-specific processing protease 26) (Deubiquitinating enzyme 26)
Index: MW: 95487 Da pI: 7.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1340.59841340.6098-8.55145230(R) (K)
1883.87001883.9503-4377921(K) (R)
2163.90662163.9731-313924090(R) (D)
2179.91212179.9680-261Met-ox 3924090(R)VTMMWQSENDSGDFYLLK(D)
2195.86582195.9629-442Met-ox 3924090(R)VTMMWQSENDSGDFYLLK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 5% (44/835AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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