MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-91
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 460 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.117e+008	13 (21)	51.0	21.7	-32.7	32.8		28804/6.7		HUMAN	Phosphoglycerate mutase 1 (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1)
2	1.116e+008	13 (21)	51.0	21.7	-32.7	32.8		28832/6.7		MOUSE	Phosphoglycerate mutase 1 (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1)
3	3.081e+004	6 (10)	42.0	10.0	6.15	73.3		24291/6.7		MOUSE	Beta crystallin B3 (Beta-B3-crystallin)
4	1.226e+004	7 (11)	7.0	11.7	-15.5	48.2		94506/5.6		HUMAN	Osmotic stress protein 94 (Heat shock 70-related protein APG-1)
5	1.201e+004	6 (10)	25.0	10.0	0.202	79.6		40833/8.3		HUMAN	LIM and cysteine-rich domains protein 1 (Dyxin)

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Detailed Results

1. 13/60 matches (21%).
Acc. #: Species: HUMAN Name: Phosphoglycerate mutase 1 (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1)
Index: MW: 28804 Da pI: 6.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1150.5955	1150.6697	-64		181	191	0	(R) (G)
1312.5406	1312.6034	-48		11	21	0	(R) (F)
1683.8546	1683.9110	-33		163	176	0	(R) (E)
1793.7422	1793.8056	-35		47	61	0	(R) (R)
1882.7779	1882.8744	-51		142	157	0	(R) (D)
1979.8190	1979.8775	-30		22	39	0	(R) (R)
2115.0269	2115.1272	-47		223	240	0	(K) (K)
2131.0666	2131.1221	-26	1Met-ox	223	240	0	(K)NLKPIKPMQFLGDEETVR(K)
2135.9378	2135.9787	-19		22	40	1	(R) (G)
2259.1723	2259.2171	-20	1Met-ox	223	241	1	(K)NLKPIKPMQFLGDEETVRK(A)
2433.0567	2433.1073	-21	1Met-ox	118	138	0	(R)SYDVPPPPMEPDHPFYSNISK(D)
2439.1091	2439.1713	-25		142	162	1	(R) (A)
2589.1976	2589.2084	-4.2	1Met-ox	117	138	1	(R)RSYDVPPPPMEPDHPFYSNISK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 51% (132/254AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/60 matches (21%).
Acc. #: Species: MOUSE Name: Phosphoglycerate mutase 1 (Phosphoglycerate mutase isozyme B) (PGAM-B) (BPG-dependent PGAM 1)
Index: MW: 28832 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1150.5955 1150.6697 -64 181 191 0 (R) (G)
1312.5406 1312.6034 -48 11 21 0 (R) (F)
1683.8546 1683.9110 -33 163 176 0 (R) (E)
1793.7422 1793.8056 -35 47 61 0 (R) (R)
1882.7779 1882.8744 -51 142 157 0 (R) (D)
1979.8190 1979.8775 -30 22 39 0 (R) (R)
2115.0269 2115.1272 -47 223 240 0 (K) (K)
2131.0666 2131.1221 -26 1Met-ox 223 240 0 (K)NLKPIKPMQFLGDEETVR(K)
2135.9378 2135.9787 -19 22 40 1 (R) (G)
2259.1723 2259.2171 -20 1Met-ox 223 241 1 (K)NLKPIKPMQFLGDEETVRK(A)
2433.0567 2433.1073 -21 1Met-ox 118 138 0 (R)SYDVPPPPMEPDHPFYSNISK(D)
2439.1091 2439.1713 -25 142 162 1 (R) (A)
2589.1976 2589.2084 -4.2 1Met-ox 117 138 1 (R)RSYDVPPPPMEPDHPFYSNISK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 51% (132/254AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Beta crystallin B3 (Beta-B3-crystallin)
Index: MW: 24291 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1150.5955 1150.5394 49 89 97 1 (R) (S)
2006.9006 2006.9415 -20 39 55 0 (R) (V)
2230.1480 2230.0239 56 129 147 0 (K) (V)
2284.1236 2284.1879 -28 148 167 1 (R) (G)
2439.1091 2439.1475 -16 1 25 1 (-) (V)
2455.1344 2455.1424 -3.3 1Met-ox 1 25 1 (-)MAEQHGAPEQAAAGKSHGGLGGSYK(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (90/211AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Osmotic stress protein 94 (Heat shock 70-related protein APG-1)
Index: MW: 94506 Da pI: 5.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1328.5476 1328.5905 -32 529 539 0 (K) (C)
1344.5273 1344.5854 -43 1Met-ox 529 539 0 (K)MQVDQEEGHQK(C)
1777.7240 1777.7703 -26 723 736 1 (K) (V)
1793.7422 1793.7652 -13 1Met-ox 723 736 1 (K)DERYDHLDPTEMEK(V)
2111.9105 2111.9676 -27 740 757 1 (K) (L)
2135.9378 2135.9192 8.7 501 519 0 (K) (N)
2151.9668 2151.9141 25 1Met-ox 501 519 0 (K)QNLEGDHSDAPMETETSFK(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (62/839AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: LIM and cysteine-rich domains protein 1 (Dyxin)
Index: MW: 40833 Da pI: 8.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1683.8546 1683.8456 5.3 278 290 0 (K) (D)
2135.9378 2135.8974 19 52 68 1 (K) (I)
2145.0857 2144.9871 46 291 307 1 (K) (C)
2181.0049 2181.1556 -69 pyroGlu 188 209 1 (K)QYKSEALGVGEVALPGQGGLPK(E)
2198.2220 2198.1821 18 188 209 1 (K) (E)
2447.1094 2447.1538 -18 214 237 0 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (93/365AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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