MS-Fit Search Results


.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-105
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 100000 Da) selects 104019 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 12734 entries.
Pre searches select 12734 entries.

Data Set 1 Results

MS-Fit search selects 168 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/43(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.797e+0049 (20)39.020.9-27.520.8 22836/6.5HUMANProteasome subunit beta type 2 (Proteasome component C7-I) (Macropain subunit C7-I) (Multicatalytic endopeptidase complex subunit C7-I)
251597 (16)30.016.3-27.924.0 22907/6.5MOUSEProteasome subunit beta type 2 (Proteasome component C7-I) (Macropain subunit C7-I) (Multicatalytic endopeptidase complex subunit C7-I)
36985 (11)12.011.610.446.7 44059/6.9MOUSELymphoid enhancer binding factor 1 (LEF-1)
43185 (11)18.011.6-21.247.9 40871/9.4HUMANRNA 3'-terminal phosphate cyclase-like protein (HSPC338)
52494 (9)7.09.3-28.335.9 87799/6.7HUMANGlutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
24813...x..xx...x.....xx....x......x....x.......
23560...x..xx...x.....x.....x...........x.......
18563......x.x..............xx.........x........
23592............x...................x.x....x.x.
58413.....xx...............................x..x.


Detailed Results

1. 9/43 matches (20%).
Acc. #: Species: HUMAN Name: Proteasome subunit beta type 2 (Proteasome component C7-I) (Macropain subunit C7-I) (Multicatalytic endopeptidase complex subunit C7-I)
Index: MW: 22836 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
875.4048875.4409-4187930(R) (S)
1000.47331000.5104-371461530(R) (E)
1031.50851031.5420-3386931(R) (S)
1306.74541306.7523-5.31711810(R) (I)
1462.80621462.8534-321701811(K) (I)
1469.69991469.7389-261861980(K) (Q)
1611.74231611.7767-2171850(R) (R)
1938.97931939.0289-261821981(R) (Q)
2020.01382020.0656-261271450(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (79/201AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/43 matches (16%).
Acc. #: Species: MOUSE Name: Proteasome subunit beta type 2 (Proteasome component C7-I) (Macropain subunit C7-I) (Multicatalytic endopeptidase complex subunit C7-I)
Index: MW: 22907 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
875.4048875.4409-4187930(R) (S)
1000.47331000.5104-371461530(R) (E)
1031.50851031.5420-3386931(R) (S)
1306.74541306.7523-5.31711810(R) (V)
1462.80621462.8534-321701811(K) (V)
1611.74231611.7767-2171850(R) (R)
2020.01382020.0656-261271450(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (62/201AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/43 matches (11%).
Acc. #: Species: MOUSE Name: Lymphoid enhancer binding factor 1 (LEF-1)
Index: MW: 44059 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1000.47331000.4852-12941020(K) (G)
1045.52321045.48024126341(K) (I)
1611.74231611.696129pyroGlu 1Met-ox 2252390(R)QPYPSSLSGDTSMSR(F)
1612.72961612.72771.22252390(R) (F)
2012.96692012.9817-7.335520(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (51/397AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/43 matches (11%).
Acc. #: Species: HUMAN Name: RNA 3'-terminal phosphate cyclase-like protein (HSPC338)
Index: MW: 40871 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1308.72721308.7891-471761870(K) (I)
1940.92581940.91117.61170(-) (Q)
2012.96692013.0149-241Met-ox 3523701(K)GGDKVLMTCVGIGFSNLSK(T)
2211.06202211.1524-412142311(R) (G)
2225.06752225.0708-1.51191(-) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (68/373AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/43 matches (9%).
Acc. #: Species: HUMAN Name: Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Index: MW: 87799 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
934.5314934.5726-445165230(R) (R)
1000.47331000.5063-335595670(R) (A)
2197.04482197.0502-2.53143310(K) (V)
2225.06752225.1422-345395580(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (55/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.