MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-107
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 908 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	7.561e+005	9 (15)	44.0	15.0	-51.3	25.5		24722/8.3		HUMAN	Superoxide dismutase [Mn], mitochondrial precursor
2	3.620e+004	8 (13)	18.0	13.3	-16.7	90.0		61164/6.3		HUMAN	Zinc finger imprinted 2
3	3.035e+004	7 (11)	8.0	11.7	-14.2	95.1		94985/5.1		HUMAN	Putative suppressor of tumorigenicity ST7
4	1.947e+004	5 (8)	21.0	8.3	0.223	104		25989/5.6		MOUSE	Ras-related protein Rab-20
5	1.247e+004	6 (10)	12.0	10.0	-57.0	60.5		70776/9.0		MOUSE	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3 (MAPK/ERK KINASE KINASE 3) (MEK KINASE 3) (MEKK 3)

82550	.	.	.	.	.	.	x	.	x	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.
86598	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.
115501	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	x	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.
9359	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
70161	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: Superoxide dismutase [Mn], mitochondrial precursor
Index: MW: 24722 Da pI: 8.3

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1004.4904	1004.5529	-62		195	202	0	(K) (A)
1028.5329	1028.6104	-75		115	123	1	(K) (D)
1090.4545	1090.5209	-61		124	132	1	(R) (E)
1424.7343	1424.8113	-54		76	89	0	(K) (F)
1738.7800	1738.8513	-41		54	68	0	(K) (Y)
1743.8035	1743.8818	-45		203	216	0	(K) (Y)
2034.9458	2035.0401	-46		135	154	0	(K) (E)
2320.0958	2320.1838	-38		135	156	1	(K) (G)
2542.1699	2542.2690	-39		54	75	1	(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 44% (98/222AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Zinc finger imprinted 2
Index: MW: 61164 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
894.3762 894.3740 2.5 383 390 0 (K) (K)
1089.3611 1089.4489 -81 282 290 0 (R) (S)
1687.8252 1687.9271 -60 129 144 0 (K) (R)
1759.7942 1759.8186 -14 114 127 0 (R) (K)
1763.7532 1763.8362 -47 360 373 0 (K) (H)
1775.8030 1775.8135 -5.9 1Met-ox 114 127 0 (R)KPHNTIQDNMENYR(K)
2098.9593 2098.8316 61 458 473 1 (K) (V)
2283.0957 2283.0716 11 1Met-ox 145 164 1 (K)RNTEMLDNLPSAGSQFPDFK(H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (97/527AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Putative suppressor of tumorigenicity ST7
Index: MW: 94985 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1090.4545 1090.4918 -34 141 149 0 (R) (K)
1109.4622 1109.5566 -85 74 81 0 (K) (A)
1187.5251 1187.5584 -28 306 316 0 (K) (I)
1449.6613 1449.5792 57 395 405 0 (R) (D)
1757.8002 1757.7351 37 447 459 0 (K) (N)
2299.0840 2299.1166 -14 614 636 0 (R) (E)
2542.1699 2542.2497 -31 612 636 1 (R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (77/859AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/60 matches (8%).
Acc. #: Species: MOUSE Name: Ras-related protein Rab-20
Index: MW: 25989 Da pI: 5.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1743.8035 1743.7392 37 170 184 0 (R) (T)
1757.8002 1757.9226 -70 29 44 1 (R) (Q)
1759.7942 1759.7341 34 1Met-ox 170 184 0 (R)EMPAAEQMCFETSAK(T)
1775.8030 1775.7290 42 2Met-ox 170 184 0 (R)EMPAAEQMCFETSAK(T)
2211.0193 2211.1120 -42 95 114 0 (R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (51/233AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3 (MAPK/ERK KINASE KINASE 3) (MEK KINASE 3) (MEKK 3)
Index: MW: 70776 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1350.5966 1350.6874 -67 514 525 0 (R) (S)
1424.7343 1424.7358 -1.1 162 174 1 (R) (S)
1685.7509 1685.8647 -67 438 452 0 (K) (D)
1701.7336 1701.8596 -74 1Met-ox 438 452 0 (K)ILTIFMEYMPGGSVK(D)
2050.8915 2050.9868 -46 314 332 0 (R) (G)
2082.8895 2083.0685 -86 579 597 0 (K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (78/626AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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