MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-112
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( MAMMALS ) selects 22098 entries.
Combined molecular weight, pI and species searches select 19837 entries.
Pre searches select 19837 entries.

Data Set 1 Results

MS-Fit search selects 1613 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/80(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	8.922e+007	11 (13)	29.0	13.8	-63.0	41.1	46675/4.8	SHEEP	Keratin, type I microfibrillar 48 kDa, component 8C-1 (Low-sulfur keratin)
2	1.465e+006	9 (11)	21.0	11.3	-68.3	25.9	46063/4.9	SHEEP	Keratin, type I microfibrillar, 47.6 kDa (Low-sulfur keratin)
3	1.071e+006	12 (15)	28.0	15.0	-78.3	34.6	53682/5.5	SHEEP	Keratin, type II microfibrillar, component 7C
4	9.093e+004	8 (10)	19.0	10.0	-71.6	64.9	77009/6.7	MOUSE	Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (Hexosephosphate aminotransferase 2) (D-fructose-6-phosphate amidotransferase 2) (GFAT 2) (GFAT2)
5	6.600e+004	11 (13)	21.0	13.8	-85.4	50.4	55256/6.1	SHEEP	Keratin, type II microfibrillar, component 5

42289	.	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	x	x	.	.	x	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
72169	.	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	x	x	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
89633	.	x	.	x	x	x	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
23049	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
94235	.	.	.	x	x	x	.	.	.	.	x	x	x	x	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 11/80 matches (13%).
Acc. #: Species: SHEEP Name: Keratin, type I microfibrillar 48 kDa, component 8C-1 (Low-sulfur keratin)
Index: MW: 46675 Da pI: 4.8

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1109.4403	1109.5267	-78		241	248	0	(R) (Q)
1153.4275	1153.5312	-90		57	65	0	(K) (L)
1169.4530	1169.5261	-63	1Met-ox	57	65	0	(K)ETMQFLNDR(L)
1449.6254	1449.7450	-82		229	240	1	(R) (D)
1478.6008	1478.6975	-65		293	305	0	(R) (Y)
1487.6561	1487.7494	-63	pyroGlu	334	345	0	(R)QNQEYQVLLDVR(A)
1504.6726	1504.7760	-69		334	345	0	(R) (A)
1625.7416	1625.8499	-67		208	222	0	(R) (V)
1834.8865	1834.9775	-50		277	292	0	(R) (D)
2157.8824	2158.0028	-56		91	107	0	(R) (T)
2348.1489	2348.1768	-12		256	275	0	(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (122/412AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/80 matches (11%).
Acc. #: Species: SHEEP Name: Keratin, type I microfibrillar, 47.6 kDa (Low-sulfur keratin)
Index: MW: 46063 Da pI: 4.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1109.4403 1109.5267 -78 242 249 0 (R) (Q)
1153.4275 1153.5312 -90 58 66 0 (K) (L)
1169.4530 1169.5261 -63 1Met-ox 58 66 0 (K)ETMQFLNDR(L)
1449.6254 1449.7450 -82 230 241 1 (R) (D)
1478.6008 1478.6975 -65 294 306 0 (R) (Y)
1487.6561 1487.7494 -63 pyroGlu 335 346 0 (R)QNQEYQVLLDVR(A)
1504.6726 1504.7760 -69 335 346 0 (R) (A)
1834.8865 1834.9775 -50 278 293 0 (R) (D)
2157.8824 2158.0028 -56 92 108 0 (R) (T)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1109.4403	1109.5267	-78		242	249	0	(R) (Q)
1153.4275	1153.5312	-90		58	66	0	(K) (L)
1169.4530	1169.5261	-63	1Met-ox	58	66	0	(K)ETMQFLNDR(L)
1449.6254	1449.7450	-82		230	241	1	(R) (D)
1478.6008	1478.6975	-65		294	306	0	(R) (Y)
1487.6561	1487.7494	-63	pyroGlu	335	346	0	(R)QNQEYQVLLDVR(A)
1504.6726	1504.7760	-69		335	346	0	(R) (A)
1834.8865	1834.9775	-50		278	293	0	(R) (D)
2157.8824	2158.0028	-56		92	108	0	(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (87/404AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/80 matches (15%).
Acc. #: Species: SHEEP Name: Keratin, type II microfibrillar, component 7C
Index: MW: 53682 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
952.4078 952.5005 -97 141 147 0 (K) (Q)
973.3928 973.4954 -105 412 419 0 (R) (L)
1011.3849 1011.4536 -68 294 301 0 (R) (S)
1011.3849 1011.4570 -71 217 224 0 (K) (K)
1038.4198 1038.4856 -63 283 291 0 (K) (S)
1245.5462 1245.6439 -78 321 330 1 (R) (V)
1254.5015 1254.5867 -68 292 301 1 (R) (S)
1263.5883 1263.6949 -84 400 410 0 (K) (R)
1549.6066 1549.7368 -84 14 27 0 (R) (C)
2053.7153 2053.9357 -107 1Met-ox 266 282 1 (K)MDNSRDLNMDCIVAEIK(A)
2112.9245 2113.0525 -61 349 368 0 (K) (C)
2191.0146 2191.1247 -50 225 243 1 (R) (R)
2371.9923 2372.1808 -79 391 410 1 (K) (R)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
952.4078	952.5005	-97		141	147	0	(K) (Q)
973.3928	973.4954	-105		412	419	0	(R) (L)
1011.3849	1011.4536	-68		294	301	0	(R) (S)
1011.3849	1011.4570	-71		217	224	0	(K) (K)
1038.4198	1038.4856	-63		283	291	0	(K) (S)
1245.5462	1245.6439	-78		321	330	1	(R) (V)
1254.5015	1254.5867	-68		292	301	1	(R) (S)
1263.5883	1263.6949	-84		400	410	0	(K) (R)
1549.6066	1549.7368	-84		14	27	0	(R) (C)
2053.7153	2053.9357	-107	1Met-ox	266	282	1	(K)MDNSRDLNMDCIVAEIK(A)
2112.9245	2113.0525	-61		349	368	0	(K) (C)
2191.0146	2191.1247	-50		225	243	1	(R) (R)
2371.9923	2372.1808	-79		391	410	1	(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (142/491AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/80 matches (10%).
Acc. #: Species: MOUSE Name: Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (Hexosephosphate aminotransferase 2) (D-fructose-6-phosphate amidotransferase 2) (GFAT 2) (GFAT2)
Index: MW: 77009 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
968.3936 968.4478 -56 666 673 0 (R) (N)
1504.6726 1504.8375 -110 345 358 0 (R) (D)
1543.6455 1543.7658 -78 80 92 0 (K) (W)
1763.7024 1763.8435 -80 1 14 1 (-) (T)
2296.9368 2297.2141 -121 274 294 1 (R) (V)
2307.0300 2307.1006 -31 30 50 1 (R) (E)
2334.0463 2334.1254 -34 155 173 1 (K) (V)
2371.9923 2372.1444 -64 415 435 0 (R) (Y)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
968.3936	968.4478	-56	666	673	0	(R) (N)
1504.6726	1504.8375	-110	345	358	0	(R) (D)
1543.6455	1543.7658	-78	80	92	0	(K) (W)
1763.7024	1763.8435	-80	1	14	1	(-) (T)
2296.9368	2297.2141	-121	274	294	1	(R) (V)
2307.0300	2307.1006	-31	30	50	1	(R) (E)
2334.0463	2334.1254	-34	155	173	1	(K) (V)
2371.9923	2372.1444	-64	415	435	0	(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (131/682AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/80 matches (13%).
Acc. #: Species: SHEEP Name: Keratin, type II microfibrillar, component 5
Index: MW: 55256 Da pI: 6.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.3928 973.4954 -105 425 432 0 (R) (L)
1011.3849 1011.4536 -68 307 314 0 (R) (S)
1038.4198 1038.4856 -63 296 304 0 (K) (S)
1153.4275 1153.5676 -121 229 237 1 (K) (K)
1153.4275 1153.5676 -121 230 238 1 (K) (S)
1169.4530 1169.5492 -82 154 161 0 (K) (C)
1245.5462 1245.6439 -78 334 343 1 (R) (V)
1254.5015 1254.5867 -68 305 314 1 (R) (S)
1263.5883 1263.6949 -84 413 423 0 (K) (R)
1526.5495 1526.7208 -112 27 39 1 (K) (G)
1834.8865 1834.9598 -40 54 71 1 (R) (A)
2371.9923 2372.1808 -79 404 423 1 (K) (R)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
973.3928	973.4954	-105	425	432	0	(R) (L)
1011.3849	1011.4536	-68	307	314	0	(R) (S)
1038.4198	1038.4856	-63	296	304	0	(K) (S)
1153.4275	1153.5676	-121	229	237	1	(K) (K)
1153.4275	1153.5676	-121	230	238	1	(K) (S)
1169.4530	1169.5492	-82	154	161	0	(K) (C)
1245.5462	1245.6439	-78	334	343	1	(R) (V)
1254.5015	1254.5867	-68	305	314	1	(R) (S)
1263.5883	1263.6949	-84	413	423	0	(K) (R)
1526.5495	1526.7208	-112	27	39	1	(K) (G)
1834.8865	1834.9598	-40	54	71	1	(R) (A)
2371.9923	2372.1808	-79	404	423	1	(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (106/502AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):