MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D114
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 166 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/53(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.745e+010	15 (28)	48.0	28.3	1.89	13.5		35993/8.7		MOUSE	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
2	1.678e+010	15 (28)	46.0	28.3	1.89	13.5		37430/9.0		HUMAN	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
3	6489	9 (16)	32.0	17.0	1.63	33.9		36054/8.6		HUMAN	Glyceraldehyde 3-phosphate dehydrogenase, liver
4	4076	6 (11)	10.0	11.3	-0.709	43.3		78011/8.7		HUMAN	Cytoplasmic tyrosine-protein kinase BMX (Bone marrow kinase BMX) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38)
5	1925	4 (7)	27.0	7.5	-10.7	68.0		16841/9.3		HUMAN	Ribonuclease 4 precursor (RNase 4)

81777	x	x	x	x	x	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	x	.	x	x	.	.	.	.	.	.	.	.	x	x	.	x	.	.	.	.	.	.	x	.	.
119594	x	x	x	x	x	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	x	.	x	x	.	.	.	.	.	.	.	.	x	x	.	x	.	.	.	.	.	.	x	.	.
117116	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	x
36351	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
93711	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 15/53 matches (28%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 35993 Da pI: 8.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1013.4374	1013.4441	-6.6		192	201	0	(R) (G)
1050.4356	1050.4420	-6.1		118	125	0	(R) (I)
1087.4826	1087.4849	-2.1		27	34	0	(R) (L)
1188.6492	1188.6476	1.3		126	135	0	(K) (Q)
1377.6317	1377.6300	1.2		202	216	0	(R) (G)
1410.6831	1410.6878	-3.4		162	173	0	(K) (K)
1695.7690	1695.7655	2.0		142	156	0	(R) (I)
1798.9041	1798.9227	-10		11	26	0	(K) (N)
1851.8871	1851.8666	11		141	156	1	(K) (I)
1879.9843	1879.9666	9.4		102	117	1	(K) (D)
1927.0298	1927.0177	6.3		10	26	1	(R) (N)
2189.9077	2189.9059	0.83		314	338	0	(R) (S)
2205.9222	2205.9008	9.7	1Met-ox	314	338	0	(R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.0959	2220.0712	11		118	135	1	(R) (Q)
2495.0499	2495.0401	3.9		227	254	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (165/341AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/53 matches (28%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 37430 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1013.4374 1013.4441 -6.6 204 213 0 (R) (G)
1050.4356 1050.4420 -6.1 130 137 0 (R) (I)
1087.4826 1087.4849 -2.1 39 46 0 (R) (L)
1188.6492 1188.6476 1.3 138 147 0 (K) (Q)
1377.6317 1377.6300 1.2 214 228 0 (R) (G)
1410.6831 1410.6878 -3.4 174 185 0 (K) (K)
1695.7690 1695.7655 2.0 154 168 0 (R) (I)
1798.9041 1798.9227 -10 23 38 0 (K) (N)
1851.8871 1851.8666 11 153 168 1 (K) (I)
1879.9843 1879.9666 9.4 114 129 1 (K) (D)
1927.0298 1927.0177 6.3 22 38 1 (R) (N)
2189.9077 2189.9059 0.83 326 350 0 (R) (S)
2205.9222 2205.9008 9.7 1Met-ox 326 350 0 (R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.0959 2220.0712 11 130 147 1 (R) (Q)
2495.0499 2495.0401 3.9 239 266 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 46% (165/353AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/53 matches (16%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1544.8141 1544.8107 2.2 235 248 0 (R) (L)
1613.9097 1613.9015 5.1 67 80 0 (K) (D)
1763.8094 1763.8029 3.7 310 323 0 (K) (V)
1861.9756 1861.9516 13 146 162 0 (K) (V)
2041.1060 2041.1082 -1.1 67 84 1 (K) (I)
2229.0126 2229.1048 -41 1Met-ox 119 139 0 (R)VIISAPSADAPMFVMGVNHEK(Y)
2245.1310 2245.0997 14 2Met-ox 119 139 0 (R)VIISAPSADAPMFVMGVNHEK(Y)
2401.2305 2401.2008 12 2Met-ox 118 139 1 (K)RVIISAPSADAPMFVMGVNHEK(Y)
2611.3735 2611.3554 6.9 1Met-ox 163 186 0 (K)VIHDNFGIVEGLMTTVHAITATQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (109/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/53 matches (11%).
Acc. #: Species: HUMAN Name: Cytoplasmic tyrosine-protein kinase BMX (Bone marrow kinase BMX) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38)
Index: MW: 78011 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1377.6317 1377.6228 6.5 238 247 0 (R) (K)
1410.6831 1410.6846 -1.1 578 588 0 (K) (Y)
1613.9097 1613.8539 35 399 413 0 (K) (R)
1777.8389 1777.8920 -30 373 387 0 (K) (L)
1927.0298 1927.0289 0.45 396 413 1 (K) (R)
2042.1098 2042.1398 -15 656 672 1 (K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (71/675AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/53 matches (7%).
Acc. #: Species: HUMAN Name: Ribonuclease 4 precursor (RNase 4)
Index: MW: 16841 Da pI: 9.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1087.4826 1087.5029 -19 97 105 0 (K) (V)
1704.7680 1704.8498 -48 70 82 0 (R) (S)
1851.8871 1851.9063 -10 131 147 0 (R) (-)
2008.0760 2008.0075 34 130 147 1 (R) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (40/147AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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