MS-Fit Search Results

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Sample ID (comment): HSGA04
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 4098 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	75.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.87e+011	31/100 (31%)	83295.0 / 4.97	HUMAN	HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (HSP 90)
2	7.24e+010	32/100 (32%)	84674.5 / 4.94	HUMAN	HEAT SHOCK PROTEIN HSP 90-ALPHA (HSP 86)
3	1.05e+010	29/100 (29%)	83326.0 / 4.97	MOUSE	HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (TUMOR SPECIFIC TRANSPLANTATION 84 KD ANTIGEN) (TSTA)
4	1.75e+009	29/100 (29%)	84788.6 / 4.93	MOUSE	HEAT SHOCK PROTEIN HSP 90-ALPHA (HSP 86) (TUMOR SPECIFIC TRANSPLANTATION 86 KD ANTIGEN) (TSTA)
5	9.56e+004	12/100 (12%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)

Detailed Results

1. 31/100 matches (31%). 83295.0 Da, pI = 4.97. Acc. # P08238. HUMAN. HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (HSP 90).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
515.3591	515.3669	-15.2836	403	406	(K)(N)
585.3885	585.4088	-34.7526	143	147	(K)(K)
617.3318	617.3510	-31.1392	569	573	(K)(K)
677.4428	677.3986	65.1290	37	41	(K)(E)
730.4891	730.4463	58.5954	451	456	(R)(Y)
829.5871	829.5511	43.4179	198	204	(R)(H)
829.5871	829.5300	68.8905	331	337	(R)(R)
901.5803	901.5260	60.3116	285	291	(K)(N)
1151.6392	1151.5584	70.1090	276	284	(K)(T)
1194.7008	1194.6483	43.9481	73	82	(K)(T)
1236.6927	1236.6377	44.4983	338	347	(R)(K)
1249.6842	1249.6177	53.2374	492	502	(K)(V)
1311.6294	1311.5705	44.9296	187	196	(K)(R)
1348.7368	1348.6650	53.2674	320	330	(K)(A)
1364.7901	1364.7327	42.0714	338	348	(R)(K)
1513.8596	1513.7862	48.4708	379	392	(R)(E)
1527.8061	1527.7443	40.4487	307	319	(K)(H)
1544.8697	1544.8284	26.7249	42	55	(R)(Y)
1782.9882	1782.9503	21.2894	625	639	(K)(Q)
1808.9795	1808.9394	22.1474	54	69	(K)(E)
1808.9795	1808.9587	11.4897	205	219	(K)(E)
1808.9795	1808.9844	-2.6922	436	450	(K)(L)
1847.8449	1847.7976	25.6132	292	306	(R)(S)
1911.1250	1911.0452	41.7436	624	639	(K)(Q)
1994.0435	1993.9202	61.8589	250	267	(K)(K)
1994.0435	1994.0819	-19.2193	558	573	(K)AKFENLCKLMKEILDK(K)	1Cys-am
2015.1224	2015.0449	38.4396	181	196	(K)(R)
2116.1220	2116.0272	44.7941	411	427	(K)(K)
2116.1220	2116.0272	44.7941	412	428	(K)(F)
2177.0216	2176.9457	34.8462	457	475	(R)(M)
2193.0398	2192.9406	45.2446	457	475	(R)YHTSQSGDEMTSLSEYVSR(M)	1Met-ox
2256.0247	2255.9594	28.9385	149	168	(K)(A)
2384.0231	2384.0543	-13.1137	148	168	(R)(A)
2388.2000	2388.1620	15.9123	360	378	(R)VFIMDSCDELIPEYLNFIR(G)	1Cys-am
2404.2445	2404.1569	36.4484	360	378	(R)VFIMDSCDELIPEYLNFIR(G)	1Met-ox 1Cys-am
2460.1757	2460.1362	16.0919	584	604	(R)LVSSPCCIVTSTYGWTANMER(I)	2Cys-am

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 49% (357/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 31.0 Mean Error = 31.5973 ppm Data Tolerance = 55.2181 ppm Mean Missed Cleavages = 0.7

2. 32/100 matches (32%). 84674.5 Da, pI = 4.94. Acc. # P07900. HUMAN. HEAT SHOCK PROTEIN HSP 90-ALPHA (HSP 86).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3591 515.3669 -15.2836 411 414 (K)(N)
617.3318 617.3510 -31.1392 577 581 (K)(K)
677.4428 677.3986 65.1290 42 46 (K)(E)
730.4891 730.4463 58.5954 459 464 (K)(Y)
815.5692 815.5143 67.2886 339 345 (R)(R)
901.5803 901.5260 60.3116 293 299 (K)(N)
1108.6062 1108.5427 57.2068 347 355 (R)(K)
1151.6392 1151.5584 70.1090 284 292 (K)(T)
1224.6985 1224.6265 58.8495 490 499 (K)(D)
1235.6664 1235.6020 52.0632 500 510 (K)(L)
1236.6927 1236.6377 44.4983 347 356 (R)(K)
1264.7033 1264.6438 46.9960 346 355 (R)(K)
1311.6294 1311.5705 44.9296 192 201 (K)(R)
1348.7368 1348.6650 53.2674 328 338 (K)(A)
1364.7901 1364.7327 42.0714 347 357 (R)(K)
1392.7920 1392.7388 38.1925 346 356 (R)(K)
1513.8596 1513.7862 48.4708 387 400 (R)(E)
1527.8061 1527.7443 40.4487 315 327 (K)(H)
1566.8056 1566.6998 67.5220 465 478 (R)YYTSASGDEMVSLK(D) 1Met-ox
1589.9031 1589.8764 16.7982 75 87 (K)(T)
1765.7988 1765.7616 21.0615 227 241 (R)(E)
1779.0065 1778.9481 32.8256 210 224 (K)(E)
1786.9930 1786.9452 26.7379 633 647 (K)(Q)
1833.8285 1833.7819 25.3918 300 314 (R)(S)
1915.1061 1915.0401 34.4542 632 647 (K)(Q)
2015.1224 2015.0449 38.4396 186 201 (K)(R)
2116.1220 2116.0353 40.9759 613 631 (R)IMKAQALRDNSTMGYMAAK(K) 1Met-ox
2193.0398 2192.9207 54.3490 251 269 (K)(K)
2256.0247 2255.9594 28.9385 154 173 (K)(T)
2431.2195 2431.1678 21.2616 368 386 (R)VFIMDNCEELIPEYLNFIR(G) 1Met-ox
2460.1757 2460.1362 16.0919 592 612 (R)LVTSPCCIVTSTYGWTANMER(I) 1Cys-am
2705.2676 2705.3683 -37.2234 513 534 (R)(E)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
515.3591	515.3669	-15.2836	411	414	(K)(N)
617.3318	617.3510	-31.1392	577	581	(K)(K)
677.4428	677.3986	65.1290	42	46	(K)(E)
730.4891	730.4463	58.5954	459	464	(K)(Y)
815.5692	815.5143	67.2886	339	345	(R)(R)
901.5803	901.5260	60.3116	293	299	(K)(N)
1108.6062	1108.5427	57.2068	347	355	(R)(K)
1151.6392	1151.5584	70.1090	284	292	(K)(T)
1224.6985	1224.6265	58.8495	490	499	(K)(D)
1235.6664	1235.6020	52.0632	500	510	(K)(L)
1236.6927	1236.6377	44.4983	347	356	(R)(K)
1264.7033	1264.6438	46.9960	346	355	(R)(K)
1311.6294	1311.5705	44.9296	192	201	(K)(R)
1348.7368	1348.6650	53.2674	328	338	(K)(A)
1364.7901	1364.7327	42.0714	347	357	(R)(K)
1392.7920	1392.7388	38.1925	346	356	(R)(K)
1513.8596	1513.7862	48.4708	387	400	(R)(E)
1527.8061	1527.7443	40.4487	315	327	(K)(H)
1566.8056	1566.6998	67.5220	465	478	(R)YYTSASGDEMVSLK(D)	1Met-ox
1589.9031	1589.8764	16.7982	75	87	(K)(T)
1765.7988	1765.7616	21.0615	227	241	(R)(E)
1779.0065	1778.9481	32.8256	210	224	(K)(E)
1786.9930	1786.9452	26.7379	633	647	(K)(Q)
1833.8285	1833.7819	25.3918	300	314	(R)(S)
1915.1061	1915.0401	34.4542	632	647	(K)(Q)
2015.1224	2015.0449	38.4396	186	201	(K)(R)
2116.1220	2116.0353	40.9759	613	631	(R)IMKAQALRDNSTMGYMAAK(K)	1Met-ox
2193.0398	2192.9207	54.3490	251	269	(K)(K)
2256.0247	2255.9594	28.9385	154	173	(K)(T)
2431.2195	2431.1678	21.2616	368	386	(R)VFIMDNCEELIPEYLNFIR(G)	1Met-ox
2460.1757	2460.1362	16.0919	592	612	(R)LVTSPCCIVTSTYGWTANMER(I)	1Cys-am
2705.2676	2705.3683	-37.2234	513	534	(R)(E)

The matched peptides cover 46% (338/732AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 32.0 Mean Error = 37.1759 ppm Data Tolerance = 52.5389 ppm Mean Missed Cleavages = 0.5

3. 29/100 matches (29%). 83326.0 Da, pI = 4.97. Acc. # P11499. MOUSE. HEAT SHOCK PROTEIN HSP 90-BETA (HSP 84) (TUMOR SPECIFIC TRANSPLANTATION 84 KD ANTIGEN) (TSTA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3591 515.3669 -15.2836 403 406 (K)(N)
617.3318 617.3510 -31.1392 569 573 (K)(K)
677.4428 677.3986 65.1290 37 41 (K)(E)
730.4891 730.4463 58.5954 451 456 (R)(Y)
829.5871 829.5511 43.4179 198 204 (R)(H)
901.5803 901.5260 60.3116 285 291 (K)(N)
1151.6392 1151.5584 70.1090 276 284 (K)(T)
1194.7008 1194.6483 43.9481 73 82 (K)(T)
1236.6927 1236.6377 44.4983 338 347 (R)(K)
1311.6294 1311.5705 44.9296 187 196 (K)(R)
1348.7368 1348.6650 53.2674 320 330 (K)(A)
1364.7901 1364.7327 42.0714 338 348 (R)(K)
1513.8596 1513.7862 48.4708 379 392 (R)(E)
1527.8061 1527.7443 40.4487 307 319 (K)(H)
1544.8697 1544.8284 26.7249 42 55 (R)(Y)
1782.9882 1782.9503 21.2894 625 639 (K)(Q)
1808.9795 1808.9394 22.1474 54 69 (K)(E)
1808.9795 1808.9587 11.4897 205 219 (K)(E)
1808.9795 1808.9844 -2.6922 436 450 (K)(L)
1847.8449 1847.7976 25.6132 292 306 (R)(S)
1911.1250 1911.0452 41.7436 624 639 (K)(Q)
1940.9905 1941.1034 -58.1894 169 186 (R)(E)
1994.0435 1993.9202 61.8589 250 267 (K)(K)
1994.0435 1994.0819 -19.2193 558 573 (K)AKFENLCKLMKEILDK(K) 1Cys-am
2015.1224 2015.0449 38.4396 181 196 (K)(R)
2116.1220 2116.0272 44.7941 411 427 (K)(K)
2116.1220 2116.0272 44.7941 412 428 (K)(F)
2177.0216 2176.9457 34.8462 457 475 (R)(M)
2193.0398 2193.1609 -55.2096 320 337 (K)(R)
2193.0398 2192.9406 45.2446 457 475 (R)YHTSQSGDEMTSLSEYVSR(M) 1Met-ox
2256.0247 2255.9594 28.9385 149 168 (K)(A)
2388.2000 2388.1620 15.9123 360 378 (R)VFIMDSCDELIPEYLNFIR(G) 1Cys-am
2404.2445 2404.1569 36.4484 360 378 (R)VFIMDSCDELIPEYLNFIR(G) 1Met-ox 1Cys-am
2460.1757 2460.1362 16.0919 584 604 (R)LVSSPCCIVTSTYGWTANMER(I) 2Cys-am

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Click individual mass to do a non-specific cleavage search.

The matched peptides cover 48% (352/724AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 29.0 Mean Error = 27.9365 ppm Data Tolerance = 63.7800 ppm Mean Missed Cleavages = 0.8

4. 29/100 matches (29%). 84788.6 Da, pI = 4.93. Acc. # P07901. MOUSE. HEAT SHOCK PROTEIN HSP 90-ALPHA (HSP 86) (TUMOR SPECIFIC TRANSPLANTATION 86 KD ANTIGEN) (TSTA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3591 515.3669 -15.2836 412 415 (K)(N)
617.3318 617.3510 -31.1392 578 582 (K)(K)
677.4428 677.3986 65.1290 42 46 (K)(E)
730.4891 730.4463 58.5954 460 465 (K)(Y)
815.5692 815.5143 67.2886 340 346 (R)(R)
901.5803 901.5260 60.3116 294 300 (K)(N)
1108.6062 1108.5427 57.2068 348 356 (R)(K)
1151.6392 1151.5584 70.1090 285 293 (K)(T)
1235.6664 1235.6020 52.0632 501 511 (K)(L)
1236.6927 1236.6377 44.4983 348 357 (R)(K)
1264.7033 1264.6438 46.9960 347 356 (R)(K)
1311.6294 1311.5705 44.9296 192 201 (K)(R)
1348.7368 1348.6650 53.2674 329 339 (K)(A)
1364.7901 1364.7327 42.0714 348 358 (R)(K)
1392.7920 1392.7388 38.1925 347 357 (R)(K)
1513.8596 1513.7862 48.4708 388 401 (R)(E)
1566.8056 1566.6998 67.5220 466 479 (R)YYTSASGDEMVSLK(D) 1Met-ox
1707.8260 1707.8706 -26.0997 487 500 (K)(D)
1779.0065 1778.9481 32.8256 210 224 (K)(E)
1786.9930 1786.9452 26.7379 634 648 (K)(Q)
1808.9795 1808.9394 22.1474 59 74 (K)(E)
1833.8285 1833.7819 25.3918 301 315 (R)(S)
1915.1061 1915.0401 34.4542 633 648 (K)(Q)
2015.1224 2015.0449 38.4396 186 201 (K)(R)
2116.1220 2116.0353 40.9759 614 632 (R)IMKAQALRDNSTMGYMAAK(K) 1Met-ox
2256.0247 2255.9594 28.9385 154 173 (K)(T)
2431.2195 2431.1678 21.2616 369 387 (R)VFIMDNCEELIPEYLNFIR(G) 1Met-ox
2460.1757 2460.1362 16.0919 593 613 (R)LVTSPCCIVTSTYGWTANMER(I) 1Cys-am
2705.2676 2705.3683 -37.2234 514 535 (R)(E)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
515.3591	515.3669	-15.2836	412	415	(K)(N)
617.3318	617.3510	-31.1392	578	582	(K)(K)
677.4428	677.3986	65.1290	42	46	(K)(E)
730.4891	730.4463	58.5954	460	465	(K)(Y)
815.5692	815.5143	67.2886	340	346	(R)(R)
901.5803	901.5260	60.3116	294	300	(K)(N)
1108.6062	1108.5427	57.2068	348	356	(R)(K)
1151.6392	1151.5584	70.1090	285	293	(K)(T)
1235.6664	1235.6020	52.0632	501	511	(K)(L)
1236.6927	1236.6377	44.4983	348	357	(R)(K)
1264.7033	1264.6438	46.9960	347	356	(R)(K)
1311.6294	1311.5705	44.9296	192	201	(K)(R)
1348.7368	1348.6650	53.2674	329	339	(K)(A)
1364.7901	1364.7327	42.0714	348	358	(R)(K)
1392.7920	1392.7388	38.1925	347	357	(R)(K)
1513.8596	1513.7862	48.4708	388	401	(R)(E)
1566.8056	1566.6998	67.5220	466	479	(R)YYTSASGDEMVSLK(D)	1Met-ox
1707.8260	1707.8706	-26.0997	487	500	(K)(D)
1779.0065	1778.9481	32.8256	210	224	(K)(E)
1786.9930	1786.9452	26.7379	634	648	(K)(Q)
1808.9795	1808.9394	22.1474	59	74	(K)(E)
1833.8285	1833.7819	25.3918	301	315	(R)(S)
1915.1061	1915.0401	34.4542	633	648	(K)(Q)
2015.1224	2015.0449	38.4396	186	201	(K)(R)
2116.1220	2116.0353	40.9759	614	632	(R)IMKAQALRDNSTMGYMAAK(K)	1Met-ox
2256.0247	2255.9594	28.9385	154	173	(K)(T)
2431.2195	2431.1678	21.2616	369	387	(R)VFIMDNCEELIPEYLNFIR(G)	1Met-ox
2460.1757	2460.1362	16.0919	593	613	(R)LVTSPCCIVTSTYGWTANMER(I)	1Cys-am
2705.2676	2705.3683	-37.2234	514	535	(R)(E)

The matched peptides cover 40% (298/733AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 29.0 Mean Error = 34.2817 ppm Data Tolerance = 58.4674 ppm Mean Missed Cleavages = 0.5

5. 12/100 matches (12%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
515.3591 515.3306 55.3215 404 407 (R)(L)
1127.6245 1127.5407 74.2981 289 298 (K)(V)
1179.6546 1179.6010 45.4273 377 386 (K)(H)
1231.6643 1231.6283 29.2959 356 366 (K)(A)
1300.6272 1300.5302 74.5784 258 267 (K)(N)
1383.7474 1383.6909 40.8483 186 197 (K)(V)
1475.8185 1475.7858 22.1370 200 211 (R)(W)
1475.8185 1475.7494 46.7931 212 223 (K)(T)
1716.8919 1716.8517 23.4497 418 432 (K)(G)
1765.7988 1765.7353 35.9661 46 65 (R)(S)
1994.0435 1993.9772 33.2701 224 239 (R)(R)
2015.1224 2015.1362 -6.8270 66 86 (R)(G)
2384.0231 2383.9524 29.6491 519 549 (R)(G)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
515.3591	515.3306	55.3215	404	407	(R)(L)
1127.6245	1127.5407	74.2981	289	298	(K)(V)
1179.6546	1179.6010	45.4273	377	386	(K)(H)
1231.6643	1231.6283	29.2959	356	366	(K)(A)
1300.6272	1300.5302	74.5784	258	267	(K)(N)
1383.7474	1383.6909	40.8483	186	197	(K)(V)
1475.8185	1475.7858	22.1370	200	211	(R)(W)
1475.8185	1475.7494	46.7931	212	223	(K)(T)
1716.8919	1716.8517	23.4497	418	432	(K)(G)
1765.7988	1765.7353	35.9661	46	65	(R)(S)
1994.0435	1993.9772	33.2701	224	239	(R)(R)
2015.1224	2015.1362	-6.8270	66	86	(R)(G)
2384.0231	2383.9524	29.6491	519	549	(R)(G)

The matched peptides cover 28% (184/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.0 Mean Error = 38.7852 ppm Data Tolerance = 43.7149 ppm Mean Missed Cleavages = 0.3