MS-Fit Search Results

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B68-26
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 4160 entries (results displayed for top 10 matches).

Results Summary

	MOWSE Score	#/121(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.537e+008	15 (12)	32.0	12.4	70.1	58.9	47656/4.7		HUMAN	Chromatin assembly factor 1 subunit C (CAF-1 subunit C) (Chromatin assembly factor I p48 subunit) (CAF-I 48 kDa subunit) (CAF-Ip48) (Retinoblastoma binding protein p48) (Retinoblastoma-binding protein 4) (RBBP-4) (MSI1 protein homolog)
2	1.173e+008	15 (12)	31.0	12.4	71.9	58.7	51771/4.9		MOUSE	Chromatin assembly factor 1 subunit C (CAF-1 subunit C) (Chromatin assembly factor I p48 subunit) (CAF-I 48 kDa subunit) (CAF-Ip48) (Retinoblastoma binding protein p48) (Retinoblastoma-binding protein 4) (RBBP-4)
3	2.589e+006	12 (9)	20.0	9.9	50.0	75.9	69741/5.6		MOUSE	HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK PROTEIN 70.2)
4	6.980e+005	11 (9)	22.0	9.1	67.9	30.8	49671/4.8	M	HUMAN	Class I beta tubulin. Tubulin beta-5 chain
5	6.968e+005	11 (9)	22.0	9.1	67.9	30.8	49759/4.8		HUMAN	Tubulin beta-1 chain
6	6.957e+005	12 (9)	26.0	9.9	63.6	38.0	49831/4.8		HUMAN	Tubulin beta-2 chain
7	5.433e+005	12 (9)	22.0	9.9	28.6	86.1	53925/9.6		MOUSE	Double-stranded RNA-binding protein Staufen homolog
8	4.887e+005	17 (14)	19.0	14.0	34.3	78.4	99635/5.5		HUMAN	Rod cGMP-specific 3',5'-cyclic phosphodiesterase alpha-subunit (GMP-PDE alpha) (PDE V-B1)
9	3.967e+005	12 (9)	20.0	9.9	43.0	68.0	70022/5.6		HUMAN	Heat shock-related 70 kDa protein 2 (Heat shock 70 kDa protein 2)
10	3.353e+005	18 (14)	29.0	14.9	35.8	70.2	69346/5.8		MOUSE	Ezrin (p81) (Cytovillin) (Villin 2)

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70103	.	.	.	.	x	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.
14926	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.
43754	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.
27059	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.
22033	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.
71233	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
86142	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.	x	.	.	x	.	.	.	.	.	.	.	x	x	x	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.
79083	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	x	.	.	.	.	.	x	.
108545	.	.	.	x	x	.	x	.	.	.	x	.	.	x	.	x	.	x	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	x	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.

Detailed Results

1. 15/121 matches (12%).
Acc. #: Species: HUMAN Name: Chromatin assembly factor 1 subunit C (CAF-1 subunit C) (Chromatin assembly factor I p48 subunit) (CAF-I 48 kDa subunit) (CAF-Ip48) (Retinoblastoma binding protein p48) (Retinoblastoma-binding protein 4) (RBBP-4) (MSI1 protein homolog)
Index: MW: 47656 Da pI: 4.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
894.5366	894.4573	89		16	22	0	(R) (I)
934.5595	934.4820	83		252	258	0	(K) (S)
950.5497	950.4770	77	1Met-ox	252	258	0	(K)LMIWDTR(S)
973.6276	973.5471	83		297	304	0	(K) (N)
1067.6161	1067.5234	87		121	129	0	(K) (A)
1128.6250	1128.5326	82		103	114	0	(K) (I)
1130.7139	1130.6322	72		341	349	1	(R) (I)
1251.6578	1251.5493	87		5	15	0	(K) (V)
1328.7266	1328.7690	-32		297	307	1	(K) (L)
1449.8276	1449.7234	72		132	143	0	(R) (T)
1465.8276	1465.7183	75	1Met-ox	132	143	0	(R)YMPQNPCIIATK(T)
1471.8448	1471.7321	77		144	156	0	(K) (H)
1607.8354	1607.7189	72	AcetN	1	15	1	(-)ADKEAAFDDAVEER(V)
1819.9747	1819.8397	74		157	172	0	(K) (L)
2873.5170	2873.3594	55		350	376	0	(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (138/425AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/121 matches (12%).
Acc. #: Species: MOUSE Name: Chromatin assembly factor 1 subunit C (CAF-1 subunit C) (Chromatin assembly factor I p48 subunit) (CAF-I 48 kDa subunit) (CAF-Ip48) (Retinoblastoma binding protein p48) (Retinoblastoma-binding protein 4) (RBBP-4)
Index: MW: 51771 Da pI: 4.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
894.5366 894.4573 89 16 22 0 (R) (I)
934.5595 934.4668 99 454 461 0 (R) (-)
934.5595 934.4820 83 252 258 0 (K) (S)
950.5497 950.4770 77 1Met-ox 252 258 0 (K)LMIWDTR(S)
973.6276 973.5471 83 297 304 0 (K) (N)
1067.6161 1067.5234 87 121 129 0 (K) (A)
1128.6250 1128.5326 82 103 114 0 (K) (I)
1130.7139 1130.6322 72 341 349 1 (R) (I)
1251.6578 1251.5493 87 5 15 0 (K) (V)
1328.7266 1328.7690 -32 297 307 1 (K) (L)
1449.8276 1449.7234 72 132 143 0 (R) (T)
1465.8276 1465.7183 75 1Met-ox 132 143 0 (R)YMPQNPCIIATK(T)
1471.8448 1471.7321 77 144 156 0 (K) (H)
1607.8354 1607.7189 72 AcetN 1 15 1 (-)ADKEAAFDDAVEER(V)
1819.9747 1819.8397 74 157 172 0 (K) (L)
2873.5170 2873.3594 55 350 376 0 (K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (146/461AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/121 matches (9%).
Acc. #: Species: MOUSE Name: HEAT SHOCK-RELATED 70 KD PROTEIN 2 (HEAT SHOCK PROTEIN 70.2)
Index: MW: 69741 Da pI: 5.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1050.5382 1050.5472 -8.6 130 139 0 (K) (V)
1081.6328 1081.5682 60 352 360 0 (K) (E)
1229.7044 1229.6854 15 161 172 0 (K) (I)
1301.7275 1301.6378 69 543 553 0 (K) (Q)
1309.7539 1309.6826 54 128 139 1 (K) (V)
1450.8121 1450.8058 4.3 349 360 1 (K) (E)
1471.8448 1471.7943 34 601 612 1 (K) (L)
1487.8477 1487.7018 98 38 50 0 (R) (L)
1620.9348 1620.7804 95 58 71 0 (K) (K)
1636.9248 1636.7753 91 1Met-ox 58 71 0 (K)NQVAMNPTNTIFDR(K)
1994.0764 1993.9231 77 79 94 1 (K) (V)
2873.5170 2873.4896 9.5 473 500 1 (R) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1050.5382	1050.5472	-8.6		130	139	0	(K) (V)
1081.6328	1081.5682	60		352	360	0	(K) (E)
1229.7044	1229.6854	15		161	172	0	(K) (I)
1301.7275	1301.6378	69		543	553	0	(K) (Q)
1309.7539	1309.6826	54		128	139	1	(K) (V)
1450.8121	1450.8058	4.3		349	360	1	(K) (E)
1471.8448	1471.7943	34		601	612	1	(K) (L)
1487.8477	1487.7018	98		38	50	0	(R) (L)
1620.9348	1620.7804	95		58	71	0	(K) (K)
1636.9248	1636.7753	91	1Met-ox	58	71	0	(K)NQVAMNPTNTIFDR(K)
1994.0764	1993.9231	77		79	94	1	(K) (V)
2873.5170	2873.4896	9.5		473	500	1	(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (130/633AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/121 matches (9%).
Acc. #: Species: HUMAN Name: Class I beta tubulin. Tubulin beta-5 chain
Acc. #: Species: CHICK Name: TUBULIN BETA-7 CHAIN (TUBULIN BETA 4')
Index: MW: 49671 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1039.6650 1039.5940 68 310 318 0 (R) (G)
1042.6328 1042.5355 93 351 359 0 (K) (G)
1077.6211 1077.5329 82 155 162 1 (K) (I)
1159.6806 1159.6298 44 1Met-ox 253 262 0 (K)LAVNMVPFPR(L)
1229.7044 1229.5989 86 381 390 0 (R) (R)
1245.6906 1245.5938 78 1Met-ox 381 390 0 (R)ISEQFTAMFR(R)
1258.7558 1258.6908 52 242 252 1 (R) (L)
1301.7275 1301.6378 69 47 58 0 (R) (Y)
1620.9348 1620.8361 61 263 276 0 (R) (G)
1636.9248 1636.8310 57 1Met-ox 263 276 0 (R)LHFFMPGFAPLTSR(G)
1959.0951 1958.9823 58 104 121 0 (K) (K)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1039.6650	1039.5940	68		310	318	0	(R) (G)
1042.6328	1042.5355	93		351	359	0	(K) (G)
1077.6211	1077.5329	82		155	162	1	(K) (I)
1159.6806	1159.6298	44	1Met-ox	253	262	0	(K)LAVNMVPFPR(L)
1229.7044	1229.5989	86		381	390	0	(R) (R)
1245.6906	1245.5938	78	1Met-ox	381	390	0	(R)ISEQFTAMFR(R)
1258.7558	1258.6908	52		242	252	1	(R) (L)
1301.7275	1301.6378	69		47	58	0	(R) (Y)
1620.9348	1620.8361	61		263	276	0	(R) (G)
1636.9248	1636.8310	57	1Met-ox	263	276	0	(R)LHFFMPGFAPLTSR(G)
1959.0951	1958.9823	58		104	121	0	(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (101/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/121 matches (9%).
Acc. #: Species: HUMAN Name: Tubulin beta-1 chain
Index: MW: 49759 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1039.6650 1039.5940 68 310 318 0 (R) (G)
1042.6328 1042.5355 93 351 359 0 (K) (G)
1077.6211 1077.5329 82 155 162 1 (K) (I)
1159.6806 1159.6298 44 1Met-ox 253 262 0 (K)LAVNMVPFPR(L)
1229.7044 1229.5989 86 381 390 0 (R) (R)
1245.6906 1245.5938 78 1Met-ox 381 390 0 (R)ISEQFTAMFR(R)
1258.7558 1258.6908 52 242 252 1 (R) (L)
1301.7275 1301.6378 69 47 58 0 (R) (Y)
1620.9348 1620.8361 61 263 276 0 (R) (G)
1636.9248 1636.8310 57 1Met-ox 263 276 0 (R)LHFFMPGFAPLTSR(G)
1959.0951 1958.9823 58 104 121 0 (K) (K)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1039.6650	1039.5940	68		310	318	0	(R) (G)
1042.6328	1042.5355	93		351	359	0	(K) (G)
1077.6211	1077.5329	82		155	162	1	(K) (I)
1159.6806	1159.6298	44	1Met-ox	253	262	0	(K)LAVNMVPFPR(L)
1229.7044	1229.5989	86		381	390	0	(R) (R)
1245.6906	1245.5938	78	1Met-ox	381	390	0	(R)ISEQFTAMFR(R)
1258.7558	1258.6908	52		242	252	1	(R) (L)
1301.7275	1301.6378	69		47	58	0	(R) (Y)
1620.9348	1620.8361	61		263	276	0	(R) (G)
1636.9248	1636.8310	57	1Met-ox	263	276	0	(R)LHFFMPGFAPLTSR(G)
1959.0951	1958.9823	58		104	121	0	(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (101/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 12/121 matches (9%).
Acc. #: Species: HUMAN Name: Tubulin beta-2 chain
Index: MW: 49831 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1039.6650 1039.5940 68 310 318 0 (R) (G)
1042.6328 1042.5355 93 351 359 0 (K) (G)
1077.6211 1077.5329 82 155 162 1 (K) (I)
1159.6806 1159.6298 44 1Met-ox 253 262 0 (K)LAVNMVPFPR(L)
1229.7044 1229.5989 86 381 390 0 (R) (R)
1245.6906 1245.5938 78 1Met-ox 381 390 0 (R)ISEQFTAMFR(R)
1258.7558 1258.6908 52 242 252 1 (R) (L)
1328.7266 1328.6487 59 47 58 0 (R) (Y)
1620.9348 1620.8361 61 263 276 0 (R) (G)
1636.9248 1636.8310 57 1Met-ox 263 276 0 (R)LHFFMPGFAPLTSR(G)
1707.9074 1707.8627 26 1Met-ox 283 297 0 (R)ALTVPELTQQMFDAK(N)
1959.0951 1958.9823 58 104 121 0 (K) (K)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1039.6650	1039.5940	68		310	318	0	(R) (G)
1042.6328	1042.5355	93		351	359	0	(K) (G)
1077.6211	1077.5329	82		155	162	1	(K) (I)
1159.6806	1159.6298	44	1Met-ox	253	262	0	(K)LAVNMVPFPR(L)
1229.7044	1229.5989	86		381	390	0	(R) (R)
1245.6906	1245.5938	78	1Met-ox	381	390	0	(R)ISEQFTAMFR(R)
1258.7558	1258.6908	52		242	252	1	(R) (L)
1328.7266	1328.6487	59		47	58	0	(R) (Y)
1620.9348	1620.8361	61		263	276	0	(R) (G)
1636.9248	1636.8310	57	1Met-ox	263	276	0	(R)LHFFMPGFAPLTSR(G)
1707.9074	1707.8627	26	1Met-ox	283	297	0	(R)ALTVPELTQQMFDAK(N)
1959.0951	1958.9823	58		104	121	0	(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (116/445AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 12/121 matches (9%).
Acc. #: Species: MOUSE Name: Double-stranded RNA-binding protein Staufen homolog
Index: MW: 53925 Da pI: 9.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
933.5320 933.5192 14 359 367 0 (K) (E)
949.5268 949.5141 13 1Met-ox 359 367 0 (K)ATVTAMIAR(E)
1158.6455 1158.6271 16 109 118 0 (R) (E)
1229.7044 1229.6101 77 1 10 0 (-) (M)
1245.6906 1245.6050 69 1Met-ox 1 10 0 (-)MYKPVDPHSR(M)
1434.8727 1434.7453 89 235 248 1 (K) (V)
1449.8276 1449.7412 60 253 265 0 (R) (V)
1465.8276 1465.7361 62 1Met-ox 253 265 0 (R)NAAENMLEILGFK(V)
1471.8448 1471.9113 -45 164 176 1 (R) (I)
1519.8401 1519.8484 -5.5 96 108 1 (K) (N)
1621.8775 1621.8372 25 1Met-ox 252 265 1 (K)RNAAENMLEILGFK(V)
2717.2154 2717.2987 -31 462 486 1 (K) (C)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
933.5320	933.5192	14		359	367	0	(K) (E)
949.5268	949.5141	13	1Met-ox	359	367	0	(K)ATVTAMIAR(E)
1158.6455	1158.6271	16		109	118	0	(R) (E)
1229.7044	1229.6101	77		1	10	0	(-) (M)
1245.6906	1245.6050	69	1Met-ox	1	10	0	(-)MYKPVDPHSR(M)
1434.8727	1434.7453	89		235	248	1	(K) (V)
1449.8276	1449.7412	60		253	265	0	(R) (V)
1465.8276	1465.7361	62	1Met-ox	253	265	0	(R)NAAENMLEILGFK(V)
1471.8448	1471.9113	-45		164	176	1	(R) (I)
1519.8401	1519.8484	-5.5		96	108	1	(K) (N)
1621.8775	1621.8372	25	1Met-ox	252	265	1	(K)RNAAENMLEILGFK(V)
2717.2154	2717.2987	-31		462	486	1	(K) (C)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 22% (108/487AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 17/121 matches (14%).
Acc. #: Species: HUMAN Name: Rod cGMP-specific 3',5'-cyclic phosphodiesterase alpha-subunit (GMP-PDE alpha) (PDE V-B1)
Index: MW: 99635 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
890.4271 890.4372 -11 198 205 0 (K) (R)
929.6296 929.5672 67 32 40 0 (K) (E)
977.6021 977.5308 73 448 455 0 (K) (Y)
1114.6024 1114.6294 -24 384 393 0 (K) (K)
1128.6250 1128.6992 -66 30 40 1 (R) (E)
1130.7139 1130.6244 79 1Met-ox 384 393 0 (K)NVLSMPIVNK(K)
1142.6169 1142.5767 35 1Met-ox 275 284 0 (R)YSVGLLDMTK(Q)
1156.6394 1156.5540 74 22 29 0 (K) (A)
1245.6906 1245.6744 13 pyroGlu 258 267 1 (R)QFHKALYTVR(A)
1251.6578 1251.5857 58 67 76 0 (R) (C)
1251.6578 1251.6196 30 545 554 1 (R) (K)
1254.6821 1254.6040 62 811 821 1 (K) (V)
1258.7558 1258.7193 29 1Met-ox 384 394 1 (K)NVLSMPIVNKK(E)
1320.7250 1320.6146 84 1Met-ox 373 383 0 (K)EPLDESGWMIK(N)
1350.7943 1350.7646 22 630 640 1 (R) (D)
1650.9432 1650.9332 6.1 531 544 1 (K) (F)
1836.0018 1835.9478 29 563 579 0 (R) (L)
1852.0562 1851.9427 61 1Met-ox 563 579 0 (R)HGFNVGQTMFSLLVTGK(L)
1852.0562 1852.0009 30 312 326 1 (R) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
890.4271	890.4372	-11		198	205	0	(K) (R)
929.6296	929.5672	67		32	40	0	(K) (E)
977.6021	977.5308	73		448	455	0	(K) (Y)
1114.6024	1114.6294	-24		384	393	0	(K) (K)
1128.6250	1128.6992	-66		30	40	1	(R) (E)
1130.7139	1130.6244	79	1Met-ox	384	393	0	(K)NVLSMPIVNK(K)
1142.6169	1142.5767	35	1Met-ox	275	284	0	(R)YSVGLLDMTK(Q)
1156.6394	1156.5540	74		22	29	0	(K) (A)
1245.6906	1245.6744	13	pyroGlu	258	267	1	(R)QFHKALYTVR(A)
1251.6578	1251.5857	58		67	76	0	(R) (C)
1251.6578	1251.6196	30		545	554	1	(R) (K)
1254.6821	1254.6040	62		811	821	1	(K) (V)
1258.7558	1258.7193	29	1Met-ox	384	394	1	(K)NVLSMPIVNKK(E)
1320.7250	1320.6146	84	1Met-ox	373	383	0	(K)EPLDESGWMIK(N)
1350.7943	1350.7646	22		630	640	1	(R) (D)
1650.9432	1650.9332	6.1		531	544	1	(K) (F)
1836.0018	1835.9478	29		563	579	0	(R) (L)
1852.0562	1851.9427	61	1Met-ox	563	579	0	(R)HGFNVGQTMFSLLVTGK(L)
1852.0562	1852.0009	30		312	326	1	(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 19% (165/860AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 12/121 matches (9%).
Acc. #: Species: HUMAN Name: Heat shock-related 70 kDa protein 2 (Heat shock 70 kDa protein 2)
Index: MW: 70022 Da pI: 5.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1050.5382 1050.5472 -8.6 130 139 0 (K) (V)
1081.6328 1081.5682 60 352 360 0 (K) (E)
1229.7044 1229.6854 15 161 172 0 (K) (I)
1309.7539 1309.6826 54 128 139 1 (K) (V)
1315.7666 1315.6534 86 543 553 0 (K) (Q)
1450.8121 1450.8058 4.3 349 360 1 (K) (E)
1471.8448 1471.7943 34 601 612 1 (K) (L)
1487.8477 1487.7018 98 38 50 0 (R) (L)
1819.9747 1819.9125 34 58 73 1 (K) (L)
1836.0018 1835.9074 51 1Met-ox 58 73 1 (K)NQVAMNPTNTIFDAKR(L)
1994.0764 1993.9231 77 79 94 1 (K) (V)
2873.5170 2873.4896 9.5 473 500 1 (R) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1050.5382	1050.5472	-8.6		130	139	0	(K) (V)
1081.6328	1081.5682	60		352	360	0	(K) (E)
1229.7044	1229.6854	15		161	172	0	(K) (I)
1309.7539	1309.6826	54		128	139	1	(K) (V)
1315.7666	1315.6534	86		543	553	0	(K) (Q)
1450.8121	1450.8058	4.3		349	360	1	(K) (E)
1471.8448	1471.7943	34		601	612	1	(K) (L)
1487.8477	1487.7018	98		38	50	0	(R) (L)
1819.9747	1819.9125	34		58	73	1	(K) (L)
1836.0018	1835.9074	51	1Met-ox	58	73	1	(K)NQVAMNPTNTIFDAKR(L)
1994.0764	1993.9231	77		79	94	1	(K) (V)
2873.5170	2873.4896	9.5		473	500	1	(R) (E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 20% (132/639AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 18/121 matches (14%).
Acc. #: Species: MOUSE Name: Ezrin (p81) (Cytovillin) (Villin 2)
Index: MW: 69346 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
890.4271 890.4583 -35 331 337 1 (R) (E)
894.5366 894.5453 -9.8 101 107 0 (K) (D)
916.5394 916.5216 19 64 71 1 (K) (K)
916.5394 916.5216 19 65 72 1 (K) (E)
933.5320 933.4828 53 336 342 1 (R) (E)
933.5320 933.4828 53 338 344 1 (K) (E)
949.5268 949.4777 52 1Met-ox 336 342 1 (R)EKEQMLR(E)
949.5268 949.4777 52 1Met-ox 338 344 1 (K)EQMLREK(E)
954.5282 954.5559 -29 1 8 0 (-) (V)
956.5470 956.5053 44 pyroGlu 321 328 1 (R)QQLEAEKK(R)
973.6276 973.5318 98 321 328 1 (R) (R)
976.6033 976.5468 58 28 35 0 (K) (T)
989.6029 989.5420 62 72 79 1 (R) (F)
1152.6715 1152.5901 71 351 359 1 (R) (R)
1493.8228 1493.6919 88 548 559 0 (R) (Q)
1620.9348 1620.8433 56 1Met-ox 172 184 1 (R)IQVWHAEHRGMLK(D)
1629.8298 1629.7953 21 41 53 0 (R) (G)
1651.8381 1651.8179 12 413 427 0 (K) (I)
1791.8676 1791.8708 -1.8 280 293 0 (R) (R)
1959.0951 1959.0147 41 530 546 1 (R) (R)
1963.0087 1962.9523 29 1Met-ox 194 209 0 (K)IAQDLEMYGINYFEIK(N)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
890.4271	890.4583	-35		331	337	1	(R) (E)
894.5366	894.5453	-9.8		101	107	0	(K) (D)
916.5394	916.5216	19		64	71	1	(K) (K)
916.5394	916.5216	19		65	72	1	(K) (E)
933.5320	933.4828	53		336	342	1	(R) (E)
933.5320	933.4828	53		338	344	1	(K) (E)
949.5268	949.4777	52	1Met-ox	336	342	1	(R)EKEQMLR(E)
949.5268	949.4777	52	1Met-ox	338	344	1	(K)EQMLREK(E)
954.5282	954.5559	-29		1	8	0	(-) (V)
956.5470	956.5053	44	pyroGlu	321	328	1	(R)QQLEAEKK(R)
973.6276	973.5318	98		321	328	1	(R) (R)
976.6033	976.5468	58		28	35	0	(K) (T)
989.6029	989.5420	62		72	79	1	(R) (F)
1152.6715	1152.5901	71		351	359	1	(R) (R)
1493.8228	1493.6919	88		548	559	0	(R) (Q)
1620.9348	1620.8433	56	1Met-ox	172	184	1	(R)IQVWHAEHRGMLK(D)
1629.8298	1629.7953	21		41	53	0	(R) (G)
1651.8381	1651.8179	12		413	427	0	(K) (I)
1791.8676	1791.8708	-1.8		280	293	0	(R) (R)
1959.0951	1959.0147	41		530	546	1	(R) (R)
1963.0087	1962.9523	29	1Met-ox	194	209	0	(K)IAQDLEMYGINYFEIK(N)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 29% (170/586AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):