MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): B130
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 2503 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/120(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.593e+01423 (19)43.019.2-40.938.7 56651/6.3HUMAND-3-phosphoglycerate dehydrogenase (3-PGDH)
23.679e+00715 (12)35.012.5-43.237.7 59266/6.4HUMANGlucose-6-phosphate 1-dehydrogenase (G6PD)
31.532e+00711 (9)30.09.2-9.4389.9 57933/8.7HUMANZinc finger protein clone 647
41.045e+00614 (11)20.011.7-15.680.0 99647/5.4MOUSERod cGMP-specific 3',5'-cyclic phosphodiesterase alpha-subunit (GMP-PDE alpha)
56.142e+00510 (8)19.08.3-17.574.5 71112/5.3MOUSEGoodpasture antigen-binding protein (GPBP) (Collagen type IV alpha 3 binding protein)
63.840e+0058 (6)14.06.7-19.657.2 77564/7.8HUMANProtein kinase C, eta type (nPKC-eta) (PKC-L)
73.819e+00511 (9)17.09.2-18.860.0 77974/7.9MOUSEProtein kinase C, eta type (nPKC-eta) (PKC-L)
83.416e+00513 (10)23.010.8-15.668.6 68520/5.6HUMANC-Rel proto-oncogene protein (C-Rel protein)
92.619e+0058 (6)18.06.7-43.749.4 51449/6.5MOUSED-3-phosphoglycerate dehydrogenase (3-PGDH) (A10)
102.511e+0058 (6)12.06.729.051.0 85208/9.0HUMANSemaphorin 3C precursor (Semaphorin E) (Sema E)
36592........x..x........x.x.x..x.x..x...xx....x...xx.x......................x.......x........x..x.x.x............x..x....x..
78500x...x.xx.x....xxx..............................x...x....x........x...x...x.x............................................
25321.......x..x...........x.................x...x..x.............xx...x..................................x.......x..........
89288.xx...........x.x.x.............x.......x............................x.x..........x..............xx.x............x......
76666....x..........................x..x..........x..................................x.x..x.....x.....................x..x...
82241..............x.............................................................x....x..........x.x.x...............x..x....
22961..............x...........................................................x.x....x.xx.......x.x.x................x.x....
33676......x...x............x..x..x.............x........x...........................x......xx..xx.................x.........
84630........x..x........................x.........x..x..............................x........x...........................x..
56792..x.............xx............................x......................xx.........................xx......................


Detailed Results

1. 23/120 matches (19%).
Acc. #: Species: HUMAN Name: D-3-phosphoglycerate dehydrogenase (3-PGDH)
Index: MW: 56651 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1099.53281099.6111-712372470(R) (A)
1130.52611130.6057-7059690(K) (L)
1345.68821345.7691-603523640(K) (N)
1357.64031357.6786-28pyroGlu 1201321(R)QIPQATASMKDGK(W)
1373.54571373.6279-60pyroGlu 5235330(K)QHVTEAFQFHF(-)
1390.57691390.6544-565235330(K) (-)
1426.72901426.8270-6921331(R) (Q)
1462.63251462.7034-499200(K) (K)
1486.67811486.7865-733813941(K) (L)
1488.65871488.7294-4876900(R) (K)
1590.73151590.7984-428201(R) (K)
1590.73151590.7984-429211(K) (I)
1638.73711638.7871-312953080(R) (G)
1639.82401639.9205-593653800(K) (E)
1654.70701654.7821-451Met-ox 2953080(R)CGEEIAVQFVDMVK(G)
1900.91291900.9690-3039540(K) (S)
2061.98962062.0391-242712890(R) (E)
2231.03322231.0767-192482680(R) (D)
2272.10642272.1681-274704910(R) (L)
2288.11902288.1630-191Met-ox 4704910(R)TQTSDPAMLPTMIGLLAEAGVR(L)
2288.11902288.1808-2739581(K) (V)
2304.11102304.1579-202Met-ox 4704910(R)TQTSDPAMLPTMIGLLAEAGVR(L)
2454.20252454.2550-21pyroGlu 34541(K)QNLSKEELIAELQDCEGLIVR(S)
2471.23862471.2816-1734541(K) (S)
2502.16612502.2047-152482701(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 43% (233/533AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/120 matches (12%).
Acc. #: Species: HUMAN Name: Glucose-6-phosphate 1-dehydrogenase (G6PD)
Index: MW: 59266 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
899.4950899.5427-533873931(K) (V)
1002.47801002.5525-742202270(R) (D)
1058.58481058.6362-491671750(R) (D)
1090.46551090.5091-404884970(R) (R)
1102.52881102.6049-693493570(R) (C)
1173.57381173.6380-551831920(R) (E)
1191.53021191.5686-324995080(R) (W)
1273.55831273.6217-502472570(R) (D)
1639.82401639.81883.23944071(R) (K)
1664.80621664.8648-3558720(R) (S)
1761.70011761.7801-451Met-ox 1531660(K)NIHESCMSQIGWNR(I)
1807.70411807.7887-471051200(R) (L)
1851.88131851.9250-241Met-ox 4404540(R)LILDVFCGSQMHFVR(S)
1927.87771927.9765-513313480(R) (W)
1956.88411956.9390-283713860(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (182/515AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/120 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein clone 647
Index: MW: 57933 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1090.46551090.5017-331041120(R) (Q)
1121.50281121.5703-602162250(R) (I)
1357.64031357.6244122913011(R) (R)
1576.74921576.8236-474654770(K) (I)
1600.74031600.8461-663813930(K) (V)
1639.82401639.82400.0121571691(K) (I)
1794.82651794.81556.13103240(R) (A)
1795.88411795.7743614504640(R) (A)
1820.92211820.8060644784920(R) (A)
2366.17222366.16373.64224411(R) (A)
2454.20252454.3092-4376980(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (157/510AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 14/120 matches (11%).
Acc. #: Species: MOUSE Name: Rod cGMP-specific 3',5'-cyclic phosphodiesterase alpha-subunit (GMP-PDE alpha)
Index: MW: 99647 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
986.5247986.4947301982050(K) (R)
989.5122989.4957175565620(R) (H)
1173.57381173.5752-1.167760(R) (C)
1173.57381173.6302-481Met-ox 1761860(K)NILASPIMNGK(D)
1273.55831273.6363-611Met-ox 911001(R)ADRVSLFMYR(T)
1320.56231320.6146-401Met-ox 3733830(K)EPLDESGWMIK(N)
1462.63251462.7290-666416520(R) (R)
1576.74921576.74303.94564671(K) (I)
1851.88131851.9427-331Met-ox 5635790(R)HGFNVGQTMFSLLVTGK(L)
1851.88131852.0009-653123261(R) (E)
1886.88951886.9071-9.37918060(R) (K)
2083.96122083.9793-8.71Met-ox 67831(R)DVQENLQAEKCTFNVMK(K)
2320.10822320.2189-482382571(R) (Q)
2325.12412325.0168466907070(K) (K)
2340.94042341.0117-301Met-ox 6907070(K)TYESTQEWTQYMMLEQTR(K)
2485.25772485.1016632Met-ox 6907081(K)TYESTQEWTQYMMLEQTRK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (175/859AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/120 matches (8%).
Acc. #: Species: MOUSE Name: Goodpasture antigen-binding protein (GPBP) (Collagen type IV alpha 3 binding protein)
Index: MW: 71112 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1002.47801002.4896-1249560(K) (S)
1448.61781448.6869-483333441(R) (V)
1475.67291475.6820-6.233430(K) (W)
1613.71561613.7964-5086980(R) (A)
2061.98962062.1184-624334511(R) (G)
2083.96122084.0010-19pyroGlu 1741911(R)QVDTLQKYFDVCADAVSK(D)
2134.03492134.02932.627431(R) (W)
2243.09622242.95636249661(K) (G)
2485.25772485.24315.95495700(K) (D)
2501.11512501.2380-491Met-ox 5495700(K)INIAMICQTLVSPPEGDQEISR(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (119/624AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/120 matches (6%).
Acc. #: Species: HUMAN Name: Protein kinase C, eta type (nPKC-eta) (PKC-L)
Index: MW: 77564 Da pI: 7.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1173.57381173.6533-6827351(R) (K)
1964.82711964.9257-501982121(K) (C)
2076.95642076.9822-122542700(K) (Q)
2272.10642272.1299-104284460(R) (S)
2288.11902288.1248-2.51Met-ox 4284460(R)LFFVMEFVNGGDLMFHIQK(S)
2304.11102304.1197-3.82Met-ox 4284460(R)LFFVMEFVNGGDLMFHIQK(S)
2471.23862471.3161-314574771(R) (D)
2500.23172500.1787214815011(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (102/682AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 11/120 matches (9%).
Acc. #: Species: MOUSE Name: Protein kinase C, eta type (nPKC-eta) (PKC-L)
Index: MW: 77974 Da pI: 7.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1173.57381173.6533-6827351(R) (K)
1940.88321940.8469191Met-ox 3894040(K)DVILQDDDVECTMTEK(R)
1964.82711964.9257-501992131(K) (C)
2076.95642076.9822-122552710(K) (Q)
2096.92892096.9480-9.11Met-ox 3894051(K)DVILQDDDVECTMTEKR(I)
2125.98042126.1109-611Met-ox 4564731(R)ARFYAAEIISALMFLHEK(G)
2272.10642272.1299-104294470(R) (S)
2288.11902288.1248-2.51Met-ox 4294470(R)LFFVMEFVNGGDLMFHIQK(S)
2304.11102304.1197-3.82Met-ox 4294470(R)LFFVMEFVNGGDLMFHIQK(S)
2485.25772485.3317-304584781(R) (D)
2500.23172500.1787214825021(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (121/683AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 13/120 matches (10%).
Acc. #: Species: HUMAN Name: C-Rel proto-oncogene protein (C-Rel protein)
Index: MW: 68520 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1058.58481058.6210-341141221(K) (I)
1121.50281121.5631-542202280(R) (G)
1367.64931367.6555-4.64224340(R) (T)
1383.67171383.7021-223633740(K) (E)
1426.72901426.8058-541231351(R) (Q)
1593.70451593.7623-361Met-ox 46580(R)TYPSIQIMNYYGK(G)
1699.83131699.796121pyroGlu 1361490(K)QLNDIEDCDLNVVR(L)
2061.98962061.9929-1.669851(K) (D)
2211.00732211.0287-9.72612781(K) (Y)
2227.12882227.0236471Met-ox 2612781(K)MQLRRPSDQEVSESMDFR(Y)
2243.09622243.0185352Met-ox 2612781(K)MQLRRPSDQEVSESMDFR(Y)
2272.10642272.2454-6163821(R) (D)
2457.15192457.2230-295145341(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (145/619AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/120 matches (6%).
Acc. #: Species: MOUSE Name: D-3-phosphoglycerate dehydrogenase (3-PGDH) (A10)
Index: MW: 51449 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1099.53281099.6111-711891990(R) (A)
1130.52611130.6057-7011210(K) (L)
1486.67811486.7865-733333461(R) (L)
1638.73711638.7871-312472600(R) (G)
1654.70701654.7821-451Met-ox 2472600(R)CGEEIAVQFVDMVK(G)
2061.98962062.0391-242232410(R) (E)
2231.03322231.0767-192002200(R) (D)
2502.16612502.2047-152002221(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (92/485AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/120 matches (6%).
Acc. #: Species: HUMAN Name: Semaphorin 3C precursor (Semaphorin E) (Sema E)
Index: MW: 85208 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
989.5122989.5016112462531(K) (S)
1273.55831273.5748-131071170(K) (V)
1298.62531298.5938241481580(R) (C)
1638.73711638.6429575295410(R) (F)
1851.88131851.86667.941550(K) (I)
1883.87861883.8025405145280(R) (D)
2304.11102303.9921526927100(K) (D)
2320.10822319.9871521Met-ox 6927100(K)DIMGAFSHSEMQMINQYCK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (92/751AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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