MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.
Sample ID (comment): D007
Database searched: SwissProt.7.25.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 2088 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.77e+012	31/60 (51%)	49671.3 / 4.78	MOUSE	TUBULIN BETA-5 CHAIN
2	1.43e+011	31/60 (51%)	49759.4 / 4.75	HUMAN	TUBULIN BETA-1 CHAIN
3	1.3e+010	30/60 (50%)	49831.5 / 4.79	HUMAN	TUBULIN BETA-2 CHAIN
4	4.41e+008	25/60 (41%)	49631.3 / 4.81	HUMAN	TUBULIN BETA-5 CHAIN
5	2.27e+006	21/60 (35%)	50518.3 / 4.86	HUMAN	TUBULIN BETA-4 CHAIN (TUBULIN BETA-III)

Detailed Results

1. 31/60 matches (51%). 49671.3 Da, pI = 4.78. Acc. # P05218. MOUSE. TUBULIN BETA-5 CHAIN.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1039.5944	1039.5940	0.3578	310	318	(R)(G)
1042.5304	1042.5355	-4.8937	351	359	(K)TAVCDIPPR(G)	1Cys-am
1077.5333	1077.5329	0.3802	155	162	(K)(I)
1079.4848	1079.4436	38.1751	298	306	(K)NMMAACDPR(H)	1Cys-am
1130.6003	1130.5958	3.9325	242	251	(R)(K)
1143.6306	1143.6349	-3.6882	253	262	(K)(L)
1159.6393	1159.6298	8.1831	253	262	(K)LAVNMVPFPR(L)	1Met-ox
1229.6071	1229.5989	6.7197	381	390	(R)(R)
1245.6004	1245.5938	5.3524	381	390	(R)ISEQFTAMFR(R)	1Met-ox
1258.6895	1258.6908	-0.9955	242	252	(R)(L)
1271.7511	1271.7298	16.6922	252	262	(R)(L)
1287.7425	1287.7247	13.8247	252	262	(R)KLAVNMVPFPR(L)	1Met-ox
1301.6531	1301.6378	11.7664	47	58	(R)(Y)
1385.7134	1385.7000	9.6518	380	390	(K)(R)
1385.7134	1385.7000	9.6518	381	391	(R)(K)
1401.7279	1401.6949	23.5529	380	390	(K)RISEQFTAMFR(R)	1Met-ox
1401.7279	1401.6949	23.5529	381	391	(R)ISEQFTAMFRR(K)	1Met-ox
1615.8711	1615.8365	21.4059	63	77	(R)(S)
1620.8669	1620.8361	18.9865	263	276	(R)(G)
1631.8648	1631.8314	20.4450	63	77	(R)AILVDLEPGTMDSVR(S)	1Met-ox
1636.8523	1636.8310	13.0022	263	276	(R)LHFFMPGFAPLTSR(G)	1Met-ox
1659.9167	1659.8958	12.5930	283	297	(R)(N)
1696.8650	1696.8335	18.5522	337	350	(K)(T)
1836.9571	1836.9390	9.8444	3	19	(R)EIVHIQAGQCGNQIGAK(F)	1Cys-am
1869.9852	1869.9784	3.6339	363	379	(K)(R)
1886.0239	1885.9734	26.8231	363	379	(K)MAVTFIGNSTAIQELFK(R)	1Met-ox
1959.0149	1958.9823	16.6315	104	121	(K)(K)
2124.1003	2124.0806	9.2458	1	19	(-)MREIVHIQAGQCGNQIGAK(F)	1Cys-am
2140.1336	2140.0756	27.1417	1	19	(-)MREIVHIQAGQCGNQIGAK(F)	1Met-ox 1Cys-am
2722.4162	2722.3544	22.6943	217	241	(K)LTTPTYGDLNHLVSATMSGVTTCLR(F)	1Cys-am
2738.4076	2738.3493	21.2655	217	241	(K)LTTPTYGDLNHLVSATMSGVTTCLR(F)	1Met-ox 1Cys-am
2798.3910	2798.3439	16.8380	78	103	(R)(G)
3102.4697	3102.4081	19.8674	20	46	(K)(I)
3102.4697	3102.4923	-7.2794	283	309	(R)ALTVPELTQQVFDAKNMMAACDPRHGR(Y)	2Met-ox 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 61% (273/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 51.7 Mean Error = 12.7620 ppm Data Tolerance = 20.7561 ppm Mean Missed Cleavages = 0.4

2. 31/60 matches (51%). 49759.4 Da, pI = 4.75. Acc. # P07437. HUMAN. TUBULIN BETA-1 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.5944 1039.5940 0.3578 310 318 (R)(G)
1042.5304 1042.5355 -4.8937 351 359 (K)TAVCDIPPR(G) 1Cys-am
1077.5333 1077.5329 0.3802 155 162 (K)(I)
1079.4848 1079.4436 38.1751 298 306 (K)NMMAACDPR(H) 1Cys-am
1130.6003 1130.5958 3.9325 242 251 (R)(K)
1143.6306 1143.6349 -3.6882 253 262 (K)(L)
1159.6393 1159.6298 8.1831 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.6071 1229.5989 6.7197 381 390 (R)(R)
1245.6004 1245.5938 5.3524 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.6895 1258.6908 -0.9955 242 252 (R)(L)
1271.7511 1271.7298 16.6922 252 262 (R)(L)
1287.7425 1287.7247 13.8247 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1301.6531 1301.6378 11.7664 47 58 (R)(Y)
1385.7134 1385.7000 9.6518 380 390 (K)(R)
1385.7134 1385.7000 9.6518 381 391 (R)(K)
1401.7279 1401.6949 23.5529 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.7279 1401.6949 23.5529 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1615.8711 1615.8365 21.4059 63 77 (R)(S)
1620.8669 1620.8361 18.9865 263 276 (R)(G)
1631.8648 1631.8314 20.4450 63 77 (R)AILVDLEPGTMDSVR(S) 1Met-ox
1636.8523 1636.8310 13.0022 263 276 (R)LHFFMPGFAPLTSR(G) 1Met-ox
1696.8650 1696.8335 18.5522 337 350 (K)(T)
1836.9571 1836.9390 9.8444 3 19 (R)EIVHIQAGQCGNQIGAK(F) 1Cys-am
1869.9852 1869.9784 3.6339 363 379 (K)(R)
1886.0239 1885.9734 26.8231 363 379 (K)MAVTFIGNSTAIQELFK(R) 1Met-ox
1959.0149 1958.9823 16.6315 104 121 (K)(K)
2124.1003 2124.0806 9.2458 1 19 (-)MREIVHIQAGQCGNQIGAK(F) 1Cys-am
2140.1336 2140.0756 27.1417 1 19 (-)MREIVHIQAGQCGNQIGAK(F) 1Met-ox 1Cys-am
2722.4162 2722.3003 42.5839 283 306 (R)ALTVPDLTQQVFDAKNMMAACDPR(H) 1Met-ox 1Cys-am
2738.4076 2738.2952 41.0388 283 306 (R)ALTVPDLTQQVFDAKNMMAACDPR(H) 2Met-ox 1Cys-am
2741.3509 2741.2948 20.4501 217 241 (R)LTTPTYGDLNHLVSGTMECVTTCLR(F) 1Met-ox
2798.3910 2798.3439 16.8380 78 103 (R)(G)
3102.4697 3102.4081 19.8674 20 46 (K)(I)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 60% (270/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 51.7 Mean Error = 14.8093 ppm Data Tolerance = 23.9565 ppm Mean Missed Cleavages = 0.4

3. 30/60 matches (50%). 49831.5 Da, pI = 4.79. Acc. # P05217. HUMAN. TUBULIN BETA-2 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.5944 1039.5940 0.3578 310 318 (R)(G)
1042.5304 1042.5355 -4.8937 351 359 (K)TAVCDIPPR(G) 1Cys-am
1077.5333 1077.5329 0.3802 155 162 (K)(I)
1079.4848 1079.4436 38.1751 298 306 (K)NMMAACDPR(H) 1Cys-am
1130.6003 1130.5958 3.9325 242 251 (R)(K)
1143.6306 1143.6349 -3.6882 253 262 (K)(L)
1159.6393 1159.6298 8.1831 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.6071 1229.5989 6.7197 381 390 (R)(R)
1245.6004 1245.5938 5.3524 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.6895 1258.6908 -0.9955 242 252 (R)(L)
1271.7511 1271.7298 16.6922 252 262 (R)(L)
1287.7425 1287.7247 13.8247 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1328.6990 1328.6487 37.8969 47 58 (R)(Y)
1385.7134 1385.7000 9.6518 380 390 (K)(R)
1385.7134 1385.7000 9.6518 381 391 (R)(K)
1401.7279 1401.6949 23.5529 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.7279 1401.6949 23.5529 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1601.8442 1601.8209 14.5756 63 77 (R)(S)
1620.8669 1620.8361 18.9865 263 276 (R)(G)
1636.8523 1636.8310 13.0022 263 276 (R)LHFFMPGFAPLTSR(G) 1Met-ox
1696.8650 1696.8335 18.5522 337 350 (K)(T)
1836.9571 1836.9390 9.8444 3 19 (R)EIVHLQAGQCGNQIGAK(F) 1Cys-am
1959.0149 1958.9823 16.6315 104 121 (K)(K)
2124.1003 2124.0806 9.2458 1 19 (-)MREIVHLQAGQCGNQIGAK(F) 1Cys-am
2140.1336 2140.0756 27.1417 1 19 (-)MREIVHLQAGQCGNQIGAK(F) 1Met-ox 1Cys-am
2722.4162 2722.3544 22.6943 217 241 (K)LTTPTYGDLNHLVSATMSGVTTCLR(F) 1Cys-am
2738.4076 2738.3493 21.2655 217 241 (K)LTTPTYGDLNHLVSATMSGVTTCLR(F) 1Met-ox 1Cys-am
2798.3910 2798.3439 16.8380 78 103 (R)(G)
2800.3447 2800.2778 23.9012 283 306 (R)ALTVPELTQQMFDAKNMMAACDPR(H) 3Met-ox 1Cys-am
3102.4697 3102.4746 -1.5554 283 309 (R)ALTVPELTQQMFDAKNMMAACDPRHGR(Y) 1Cys-am
3116.5442 3116.4237 38.6355 20 46 (K)(I)
3118.4069 3118.4695 -20.0599 283 309 (R)ALTVPELTQQMFDAKNMMAACDPRHGR(Y) 1Met-ox 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 57% (256/445AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 50.0 Mean Error = 13.0639 ppm Data Tolerance = 26.3823 ppm Mean Missed Cleavages = 0.5

4. 25/60 matches (41%). 49631.3 Da, pI = 4.81. Acc. # P04350. HUMAN. TUBULIN BETA-5 CHAIN.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1039.5944 1039.5940 0.3578 310 318 (R)(G)
1042.5304 1042.5355 -4.8937 351 359 (K)TAVCDIPPR(G) 1Cys-am
1079.4848 1079.4944 -8.8610 155 162 (K)(I)
1079.4848 1079.4436 38.1751 298 306 (K)NMMAACDPR(H) 1Cys-am
1130.6003 1130.5958 3.9325 242 251 (R)(K)
1143.6306 1143.6349 -3.6882 253 262 (K)(L)
1159.6393 1159.6298 8.1831 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.6071 1229.5989 6.7197 381 390 (R)(R)
1245.6004 1245.5938 5.3524 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.6895 1258.6908 -0.9955 242 252 (R)(L)
1271.7511 1271.7298 16.6922 252 262 (R)(L)
1287.7425 1287.7247 13.8247 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1385.7134 1385.7000 9.6518 380 390 (K)(R)
1385.7134 1385.7000 9.6518 381 391 (R)(K)
1401.7279 1401.6949 23.5529 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.7279 1401.6949 23.5529 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1601.8442 1601.8209 14.5756 63 77 (R)(S)
1696.8650 1696.8335 18.5522 337 350 (K)(T)
1836.9571 1836.9390 9.8444 3 19 (R)EIVHLQAGQCGNQIGAK(F) 1Cys-am
1869.9852 1869.9784 3.6339 363 379 (K)(R)
1886.0239 1885.9734 26.8231 363 379 (K)MAVTFIGNSTAIQELFK(R) 1Met-ox
2124.1003 2124.0806 9.2458 1 19 (-)MREIVHLQAGQCGNQIGAK(F) 1Cys-am
2140.1336 2140.0756 27.1417 1 19 (-)MREIVHLQAGQCGNQIGAK(F) 1Met-ox 1Cys-am
2722.4162 2722.3544 22.6943 217 241 (K)LTTPTYGDLNHLVSATMSGVTTCLR(F) 1Cys-am
2738.4076 2738.3493 21.2655 217 241 (K)LTTPTYGDLNHLVSATMSGVTTCLR(F) 1Met-ox 1Cys-am
2738.4076 2738.2774 47.5244 283 306 (R)GLTVPELTQQMFDAKNMMAACDPR(H) 1Cys-am
2798.3910 2798.3439 16.8380 78 103 (R)(G)
3104.3513 3104.4538 -33.0313 283 309 (R)GLTVPELTQQMFDAKNMMAACDPRHGR(Y) 1Met-ox 1Cys-am
3116.5442 3116.4237 38.6355 20 46 (K)(I)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

The matched peptides cover 51% (229/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 41.7 Mean Error = 12.5845 ppm Data Tolerance = 32.0826 ppm Mean Missed Cleavages = 0.4

5. 21/60 matches (35%). 50518.3 Da, pI = 4.86. Acc. # Q13509. HUMAN. TUBULIN BETA-4 CHAIN (TUBULIN BETA-III).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1079.4848 1079.4436 38.1751 298 306 (K)NMMAACDPR(H) 1Cys-am
1130.6003 1130.5958 3.9325 242 251 (R)(K)
1143.6306 1143.6349 -3.6882 253 262 (K)(L)
1159.6393 1159.6298 8.1831 253 262 (K)LAVNMVPFPR(L) 1Met-ox
1229.6071 1229.5989 6.7197 381 390 (R)(R)
1245.6004 1245.5938 5.3524 381 390 (R)ISEQFTAMFR(R) 1Met-ox
1258.6895 1258.6908 -0.9955 242 252 (R)(L)
1271.7511 1271.7298 16.6922 252 262 (R)(L)
1287.7425 1287.7247 13.8247 252 262 (R)KLAVNMVPFPR(L) 1Met-ox
1385.7134 1385.7000 9.6518 380 390 (K)(R)
1385.7134 1385.7000 9.6518 381 391 (R)(K)
1401.7279 1401.6949 23.5529 380 390 (K)RISEQFTAMFR(R) 1Met-ox
1401.7279 1401.6949 23.5529 381 391 (R)ISEQFTAMFRR(K) 1Met-ox
1615.8711 1615.8365 21.4059 63 77 (R)(S)
1631.8648 1631.8314 20.4450 63 77 (R)AILVDLEPGTMDSVR(S) 1Met-ox
1696.8650 1696.8335 18.5522 337 350 (K)(V)
1836.9571 1836.9390 9.8444 3 19 (R)EIVHIQAGQCGNQIGAK(F) 1Cys-am
1959.0149 1958.9823 16.6315 104 121 (K)(K)
2124.1003 2124.0806 9.2458 1 19 (-)MREIVHIQAGQCGNQIGAK(F) 1Cys-am
2140.1336 2140.0756 27.1417 1 19 (-)MREIVHIQAGQCGNQIGAK(F) 1Met-ox 1Cys-am
2800.3447 2800.2778 23.9012 283 306 (R)ALTVPELTQQMFDAKNMMAACDPR(H) 3Met-ox 1Cys-am
3102.4697 3102.4746 -1.5554 283 309 (R)ALTVPELTQQMFDAKNMMAACDPRHGR(Y) 1Cys-am
3118.4069 3118.4695 -20.0599 283 309 (R)ALTVPELTQQMFDAKNMMAACDPRHGR(Y) 1Met-ox 1Cys-am

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1079.4848	1079.4436	38.1751	298	306	(K)NMMAACDPR(H)	1Cys-am
1130.6003	1130.5958	3.9325	242	251	(R)(K)
1143.6306	1143.6349	-3.6882	253	262	(K)(L)
1159.6393	1159.6298	8.1831	253	262	(K)LAVNMVPFPR(L)	1Met-ox
1229.6071	1229.5989	6.7197	381	390	(R)(R)
1245.6004	1245.5938	5.3524	381	390	(R)ISEQFTAMFR(R)	1Met-ox
1258.6895	1258.6908	-0.9955	242	252	(R)(L)
1271.7511	1271.7298	16.6922	252	262	(R)(L)
1287.7425	1287.7247	13.8247	252	262	(R)KLAVNMVPFPR(L)	1Met-ox
1385.7134	1385.7000	9.6518	380	390	(K)(R)
1385.7134	1385.7000	9.6518	381	391	(R)(K)
1401.7279	1401.6949	23.5529	380	390	(K)RISEQFTAMFR(R)	1Met-ox
1401.7279	1401.6949	23.5529	381	391	(R)ISEQFTAMFRR(K)	1Met-ox
1615.8711	1615.8365	21.4059	63	77	(R)(S)
1631.8648	1631.8314	20.4450	63	77	(R)AILVDLEPGTMDSVR(S)	1Met-ox
1696.8650	1696.8335	18.5522	337	350	(K)(V)
1836.9571	1836.9390	9.8444	3	19	(R)EIVHIQAGQCGNQIGAK(F)	1Cys-am
1959.0149	1958.9823	16.6315	104	121	(K)(K)
2124.1003	2124.0806	9.2458	1	19	(-)MREIVHIQAGQCGNQIGAK(F)	1Cys-am
2140.1336	2140.0756	27.1417	1	19	(-)MREIVHIQAGQCGNQIGAK(F)	1Met-ox 1Cys-am
2800.3447	2800.2778	23.9012	283	306	(R)ALTVPELTQQMFDAKNMMAACDPR(H)	3Met-ox 1Cys-am
3102.4697	3102.4746	-1.5554	283	309	(R)ALTVPELTQQMFDAKNMMAACDPRHGR(Y)	1Cys-am
3118.4069	3118.4695	-20.0599	283	309	(R)ALTVPELTQQMFDAKNMMAACDPRHGR(Y)	1Met-ox 1Cys-am

The matched peptides cover 28% (126/450AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 35.0 Mean Error = 12.1808 ppm Data Tolerance = 24.7655 ppm Mean Missed Cleavages = 0.6