MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D23
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1104 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/84(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.020e+005	8 (9)	17.0	9.5	19.0	52.4		42824/4.6		HUMAN	Nucleosome assembly protein 1-like 4 (Nucleosome assembly protein 2) (NAP2)
2	5424	8 (9)	12.0	9.5	-5.07	55.7		64512/6.1		HUMAN	Keratin, type II cytoskeletal 3 (Cytokeratin 3) (K3) (CK3) (65 kDa cytokeratin)
3	4969	9 (10)	15.0	10.7	10.3	20.1		49631/4.8		HUMAN	Tubulin beta-5 chain
4	3502	7 (8)	19.0	8.3	9.49	49.4		46653/9.2		MOUSE	Zinc finger protein 59 (Zfp-59) (Zinc finger protein Mfg-2)
5	2386	6 (7)	11.0	7.1	25.1	39.0		57884/7.2		HUMAN	Cytochrome P450 19 (Aromatase) (CYPXIX) (Estrogen synthetase) (P-450AROM)

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Detailed Results

1. 8/84 matches (9%).
Acc. #: Species: HUMAN Name: Nucleosome assembly protein 1-like 4 (Nucleosome assembly protein 2) (NAP2)
Index: MW: 42824 Da pI: 4.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1201.5517	1201.5999	-40		27	36	0	(K) (V)
1217.6117	1217.5948	14	1Met-ox	27	36	0	(K)LTDQVMQNPR(V)
1328.7434	1328.6891	41		95	105	0	(K) (R)
1336.6513	1336.6174	25		84	93	0	(K) (K)
1464.7791	1464.7123	46		84	94	1	(K) (Y)
1484.8283	1484.7902	26		95	106	1	(K) (R)
1827.9435	1827.9070	20		283	297	0	(K) (A)
1911.0264	1910.9864	21		45	61	0	(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (65/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/84 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 3 (Cytokeratin 3) (K3) (CK3) (65 kDa cytokeratin)
Index: MW: 64512 Da pI: 6.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1045.5519 1045.5642 -12 397 406 0 (K) (H)
1139.5836 1139.5407 38 484 492 0 (R) (L)
1155.5471 1155.5356 9.9 1Met-ox 484 492 0 (R)DYQELMNVK(L)
1161.6374 1161.6380 -0.55 216 224 1 (K) (V)
1179.6164 1179.6010 13 260 269 0 (R) (G)
1284.5886 1284.6476 -46 298 308 0 (R) (K)
1697.8124 1697.8822 -41 185 199 1 (K) (E)
1911.0264 1911.0300 -1.9 464 480 1 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (81/629AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/84 matches (10%).
Acc. #: Species: HUMAN Name: Tubulin beta-5 chain
Index: MW: 49631 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1039.6016 1039.5940 7.2 310 318 0 (R) (G)
1042.5392 1042.5355 3.5 351 359 0 (K) (G)
1130.5939 1130.5958 -1.7 242 251 0 (R) (K)
1159.6330 1159.6298 2.8 1Met-ox 253 262 0 (K)LAVNMVPFPR(L)
1229.6041 1229.5989 4.3 381 390 0 (R) (R)
1245.6031 1245.5938 7.5 1Met-ox 381 390 0 (R)ISEQFTAMFR(R)
1258.7228 1258.6908 25 242 252 1 (R) (L)
1401.7119 1401.6949 12 1Met-ox 381 391 1 (R)ISEQFTAMFRR(K)
1401.7119 1401.6949 12 1Met-ox 380 390 1 (K)RISEQFTAMFR(R)
2071.1436 2071.0824 30 104 122 1 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (70/444AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/84 matches (8%).
Acc. #: Species: MOUSE Name: Zinc finger protein 59 (Zfp-59) (Zinc finger protein Mfg-2)
Index: MW: 46653 Da pI: 9.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1126.5409 1126.5138 24 56 64 1 (K) (S)
1251.6735 1251.6333 32 268 278 0 (K) (K)
1258.7228 1258.6796 34 180 189 0 (R) (T)
1354.7388 1354.7483 -7.1 316 327 0 (K) (I)
1372.7129 1372.6796 24 106 116 0 (K) (D)
1566.8070 1566.8433 -23 pyroGlu 36 48 1 (R)QAFQHPNLLKYPK(A)
1697.8124 1697.8433 -18 243 256 0 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (80/406AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/84 matches (7%).
Acc. #: Species: HUMAN Name: Cytochrome P450 19 (Aromatase) (CYPXIX) (Estrogen synthetase) (P-450AROM)
Index: MW: 57884 Da pI: 7.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1002.4718 1002.4826 -11 1Met-ox 441 448 0 (K)YIAMVMMK(A)
1215.6208 1215.5951 21 426 435 0 (R) (G)
1251.6735 1251.6230 40 486 495 0 (K) (N)
1267.6502 1267.6179 26 1Met-ox 486 495 0 (K)NMLEMIFTPR(N)
1474.8612 1474.8157 31 87 99 0 (R) (S)
2071.1436 2071.0541 43 404 420 0 (R) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (58/503AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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