MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D27
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 356 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/90(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.093e+0048 (8)17.08.9-63.729.2 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
21.647e+0045 (5)15.05.6-57.335.2 71289/6.0MOUSEGTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
321825 (5)13.05.6-56.056.2 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
417605 (5)8.05.6-46.088.1 82433/6.8HUMANATP-dependent helicase DDX1 (DEAD-box protein 1) (DEAD-box protein-retinoblastoma) (DBP-RB)
515395 (5)25.05.6-73.359.0 36939/6.0HUMANCell division protein kinase 6 (Serine/threonine-protein kinase PLSTIRE)
615366 (6)9.06.7-68.943.7 95203/8.9MOUSEGlutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
715056 (6)20.06.7-72.450.7 38897/9.0HUMANWNT-7A protein precursor
815016 (6)20.06.7-72.450.7 38989/9.0MOUSEWNT-7A protein precursor
914804 (4)11.04.4-69.976.6 61195/6.6HUMANReceptor-interacting serine/threonine protein kinase 2 (RIP-like interacting CLARP kinase) (Receptor-interacting protein 2) (RIP-2) (CARD-containing interleukin-1 beta converting enzyme associated kinase) (CARD-containing IL-1 beta ICE-kinase)
1013926 (6)13.06.7-63.142.2 58868/5.2MOUSEProto-oncogene tyrosine-protein kinase FGR (P55-FGR) (C-FGR)
45313....x............................x....................x.....x...........x.x.x...x.........
102436....................................................................xx.....x.....x.....x..
97443........................................x......................x.x...x...............x....
310..x.....................x........x...................................x.....x..............
46749..x.............................................x.........................x....x..x.......
4713....x............................................x....x.............xx.x..................
22394........................x............x........x.............x...x...x.....................
62399........................x............x........x.............x...x...x.....................
30539.....................................................x....x...x..................x........
89314.............x...................x...........................x..........x....x.....x......


Detailed Results

1. 8/90 matches (8%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1015.40371015.4406-361Met-ox 2902980(K)DVDGAYMTK(V)
1179.52471179.6010-653773860(K) (H)
1475.64911475.7494-682122230(K) (T)
1475.64911475.7858-932002110(R) (W)
1638.74961638.8604-681861991(K) (F)
1993.86701993.9772-552242390(R) (R)
2149.94642150.0783-612242401(R) (R)
2285.98402286.1254-623673861(K) (H)
2383.79632383.9524-655195490(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 17% (115/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/90 matches (5%).
Acc. #: Species: MOUSE Name: GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
Index: MW: 71289 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1838.80061838.9149-6259741(R) (E)
1851.76341851.8289-354624770(R) (T)
2211.03762211.1331-435645840(R) (G)
2417.97622418.1651-781681880(R) (F)
2873.20312873.3971-673834070(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (99/643AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/90 matches (5%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1235.47231235.5293-4646580(R) (V)
1721.63261721.7666-7846621(R) (G)
1791.69691791.7283-174905120(R) (G)
1851.76341851.9275-893213351(K) (N)
2705.02642705.1617-5063940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (87/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/90 matches (5%).
Acc. #: Species: HUMAN Name: ATP-dependent helicase DDX1 (DEAD-box protein 1) (DEAD-box protein-retinoblastoma) (DBP-RB)
Index: MW: 82433 Da pI: 6.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1005.40731005.4893-826796870(K) (V)
1109.40731109.4838-6991980(K) (G)
1179.52471179.6196-805685770(R) (G)
1851.76341851.7907-151331460(K) (V)
2211.03762211.0028165175350(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (60/740AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/90 matches (5%).
Acc. #: Species: HUMAN Name: Cell division protein kinase 6 (Serine/threonine-protein kinase PLSTIRE)
Index: MW: 36939 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1005.40731005.4853-782212300(R) (I)
1368.60521368.7388-982462571(R) (S)
2149.94642150.1021-72941110(K) (V)
2368.88002369.1043-953063261(R) (-)
2569.23642569.2986-2467871(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 25% (82/326AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 6/90 matches (6%).
Acc. #: Species: MOUSE Name: Glutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
Index: MW: 95203 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1015.40371015.4494-457057120(K) (Q)
1391.55461391.6364-596646760(R) (Q)
1475.64911475.7203-484204321(R) (T)
1838.80061838.9396-767868010(K) (N)
1851.76341851.9228-863874021(K) (I)
1940.84721941.0407-1004334480(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (82/836AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 6/90 matches (6%).
Acc. #: Species: HUMAN Name: WNT-7A protein precursor
Index: MW: 38897 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1109.40731109.5128-953123200(R) (V)
1227.46911227.5443-613283350(K) (C)
1323.52581323.6520-952132220(K) (E)
1638.74961638.8062-3582951(R) (E)
1765.64211765.8035-911982121(K) (T)
1838.80061838.9044-561741891(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (72/349AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 6/90 matches (6%).
Acc. #: Species: MOUSE Name: WNT-7A protein precursor
Index: MW: 38989 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1109.40731109.5128-953123200(R) (V)
1227.46911227.5443-613283350(K) (C)
1323.52581323.6520-952132220(K) (E)
1638.74961638.8062-3582951(R) (E)
1765.64211765.8035-911982121(K) (T)
1838.80061838.9044-561741891(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (72/349AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 4/90 matches (4%).
Acc. #: Species: HUMAN Name: Receptor-interacting serine/threonine protein kinase 2 (RIP-like interacting CLARP kinase) (Receptor-interacting protein 2) (RIP-2) (CARD-containing interleukin-1 beta converting enzyme associated kinase) (CARD-containing IL-1 beta ICE-kinase)
Index: MW: 61195 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1471.66191471.7579-653143260(K) (K)
1570.79551570.8229-171491610(K) (I)
1707.67491707.8441-994895030(R) (V)
2417.97622418.2128-982652850(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (62/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 6/90 matches (6%).
Acc. #: Species: MOUSE Name: Proto-oncogene tyrosine-protein kinase FGR (P55-FGR) (C-FGR)
Index: MW: 58868 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1057.41801057.4997-77181(-) (L)
1179.52471179.6084-712902990(K) (L)
1687.75691687.8404-492422551(K) (R)
1993.86701993.9197-26921060(K) (S)
2342.93032343.1014-732562751(R) (V)
2584.05902584.2692-812572791(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (71/517AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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