MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D60
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 430 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/40(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.323e+005	9 (22)	37.0	22.5	-35.6	28.8		19596/4.8		HUMAN	Translationally controlled tumor protein (TCTP) (p23) (Histamine-releasing factor) (HRF)
2	5792	6 (15)	13.0	15.0	-4.07	55.3		78440/6.9		HUMAN	PR-domain zinc finger protein 17
3	4098	4 (10)	15.0	10.0	-43.2	38.9		21418/4.9		HUMAN	Chromobox protein homolog 1 (Heterochromatin protein 1 homolog beta) (HP1 beta) (Modifier 1 protein) (M31) (Heterochromatin protein p25)
4	2657	4 (10)	13.0	10.0	7.95	36.5		58629/9.1		MOUSE	T-box transcription factor TBX6 (T-box protein 6)
5	2371	6 (15)	16.0	15.0	-20.1	37.0		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)

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61173	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.
45313	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x

Detailed Results

1. 9/40 matches (22%).
Acc. #: Species: HUMAN Name: Translationally controlled tumor protein (TCTP) (p23) (Histamine-releasing factor) (HRF)
Index: MW: 19596 Da pI: 4.8

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1056.4886	1056.5438	-52		103	110	0	(K) (V)
1241.6088	1241.6602	-41		101	110	1	(K) (V)
1419.6849	1419.7670	-58		111	123	0	(R) (H)
1435.7098	1435.7619	-36	1Met-ox	111	123	0	(R)VKPFMTGAAEQIK(H)
1446.6519	1446.6979	-32		153	164	0	(R) (D)
1446.6519	1446.7150	-44		22	34	0	(R) (M)
1462.6423	1462.6928	-35	1Met-ox	153	164	0	(R)EDGVTPYMIFFK(D)
1712.7679	1712.7842	-9.5		6	19	0	(R) (I)
1715.8505	1715.9002	-29		20	34	1	(K) (M)
1728.7452	1728.7791	-20	1Met-ox	6	19	0	(R)DLISHDEMFSDIYK(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (64/172AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/40 matches (15%).
Acc. #: Species: HUMAN Name: PR-domain zinc finger protein 17
Index: MW: 78440 Da pI: 6.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1307.6317 1307.6166 12 309 319 0 (K) (A)
1435.7098 1435.7116 -1.2 308 319 1 (K) (A)
1462.6423 1462.7277 -58 672 684 0 (R) (C)
1838.8897 1838.8682 12 526 540 0 (R) (R)
2211.0818 2211.0188 29 426 444 0 (K) (A)
2211.0818 2211.1068 -11 489 506 1 (R) (Q)
2284.1595 2284.1807 -9.3 542 561 1 (R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (97/720AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/40 matches (10%).
Acc. #: Species: HUMAN Name: Chromobox protein homolog 1 (Heterochromatin protein 1 homolog beta) (HP1 beta) (Modifier 1 protein) (M31) (Heterochromatin protein p25)
Index: MW: 21418 Da pI: 4.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1540.7162 1540.7470 -20 156 167 0 (K) (L)
1712.7679 1712.8603 -54 122 137 0 (R) (W)
1728.7452 1728.8552 -64 1Met-ox 122 137 0 (R)IIGATDSSGELMFLMK(W)
1744.7883 1744.8501 -35 2Met-ox 122 137 0 (R)IIGATDSSGELMFLMK(W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (28/185AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/40 matches (10%).
Acc. #: Species: MOUSE Name: T-box transcription factor TBX6 (T-box protein 6)
Index: MW: 58629 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1241.6088 1241.5650 35 321 332 0 (R) (L)
2211.0818 2211.0835 -0.75 17 37 0 (R) (Y)
2283.2129 2283.2171 -1.8 373 393 0 (R) (G)
2284.1595 2284.1616 -0.89 394 413 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (74/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/40 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1070.5028 1070.5019 0.83 pyroGlu 4 12 1 (R)QFSSRSGYR(S)
1179.5563 1179.6010 -38 377 386 0 (K) (H)
1475.7137 1475.7494 -24 212 223 0 (K) (T)
1475.7137 1475.7858 -49 200 211 0 (R) (W)
1687.9424 1687.9707 -17 66 82 1 (R) (G)
1993.9489 1993.9772 -14 224 239 0 (R) (R)
2383.9528 2383.9524 0.17 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (107/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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