MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D61
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 480 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/58(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
18.000e+0047 (12)26.012.18.2559.3 25358/4.8HUMANProteasome subunit beta type 6 precursor (Proteasome delta chain) (Macropain delta chain) (Multicatalytic endopeptidase complex delta chain) (Proteasome subunit Y)
27.108e+0047 (12)26.012.116.962.6 25425/5.0MOUSEProteasome subunit beta type 6 precursor (Proteasome delta chain) (Macropain delta chain) (Multicatalytic endopeptidase complex delta chain) (Proteasome subunit Y)
32.791e+00410 (17)13.017.21.7441.3 97657/5.5HUMANSplicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit)
42.521e+0048 (13)11.013.8-1.6270.6 96333/9.1HUMANATP-dependent RNA helicase DDX24 (DEAD-box protein 24)
51.246e+0046 (10)8.010.329.425.0 84018/6.3HUMANCullin homolog 4B (CUL-4B)
694485 (8)8.08.64.0736.3 97623/5.6HUMANCoatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)
760184 (6)9.06.940.115.9 68657/7.0HUMANProstaglandin G/H synthase 1 precursor (Cyclooxygenase -1) (COX-1) (Prostaglandin-endoperoxide synthase 1) (Prostaglandin H2 synthase 1) (PGH synthase 1) (PGHS-1) (PHS 1)
858485 (8)10.08.622.252.1 72259/5.9MOUSEHistidine ammonia-lyase (Histidase)
947217 (12)41.012.18.1060.8 21952/9.9HUMANThyroid receptor interacting protein 11 (TRIP-11)
1042917 (12)26.012.19.2558.5 44114/8.3MOUSEDual specificity mitogen-activated protein kinase kinase 4 (MAP kinase kinase 4) (MAPKK 4) (MAPK/ERK kinase 4) (JNK activating kinase 1) (C-JUN N-terminal kinase kinase 1) (JNK kinase 1) (JNKK 1) (SAPK/ERK kinase 1) (SEK1)
34938.......xxxx........x................................xx....
13906.......xxx......................................x...xx.x..
12970...........x.x......x.....x..........x..x....x...x..xx....
77647......................x..............xx..x...xx.x....x....
38192.......................x...x..........x................xxx
89213...........................x........x.x..x............x...
65289.....................................x..........x......x.x
17841...................................xx..xx.....x...........
84077.............x.xxx......x...x...........x.................
89743..................x...xx............x...x........x.....x..


Detailed Results

1. 7/58 matches (12%).
Acc. #: Species: HUMAN Name: Proteasome subunit beta type 6 precursor (Proteasome delta chain) (Macropain delta chain) (Multicatalytic endopeptidase complex delta chain) (Proteasome subunit Y)
Index: MW: 25358 Da pI: 4.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1083.53781083.5435-5.254630(R) (V)
1093.61221093.6257-12pyroGlu 2212300(R)QVLLGDQIPK(F)
1110.62111110.6523-282212300(R) (F)
1115.59911115.6061-6.32102200(R) (Q)
1294.73091294.71191564741(R) (I)
2345.18072345.072646pyroGlu 1571780(R)QSFAIGGSGSSYIYGYVDATYR(E)
2362.21482362.0992491571780(R) (E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 26% (64/239AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/58 matches (12%).
Acc. #: Species: MOUSE Name: Proteasome subunit beta type 6 precursor (Proteasome delta chain) (Macropain delta chain) (Multicatalytic endopeptidase complex delta chain) (Proteasome subunit Y)
Index: MW: 25425 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1083.53781083.5435-5.253620(R) (V)
1093.61221093.6257-12pyroGlu 2202290(R)QVLLGDQIPK(F)
1110.62111110.6523-282202290(R) (F)
1982.99501982.9316321Met-ox 1831990(K)DECLQFTANALALAMER(D)
2345.18072345.072646pyroGlu 1561770(R)QSFAIGGSGSSYIYGYVDATYR(E)
2362.21482362.0992491561770(R) (E)
2513.27652513.1838371781991(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (64/238AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/58 matches (17%).
Acc. #: Species: HUMAN Name: Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit)
Index: MW: 97657 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1125.58041125.5904-8.9AcetN 1120(-)APGAAQELQAK(L)
1129.61071129.5853225825910(K) (I)
1307.70021307.7298-236056150(K) (Y)
1540.80061540.7847104734841(R) (R)
1936.05391935.9897331761930(K) (T)
1952.00331951.98469.61Met-ox 1761930(K)MGTPVPRPPQDMGQIGVR(T)
1967.04051967.0674-1437541(R) (A)
1994.08191994.06717.413301(K) (L)
2345.18072345.2577-33AcetN 1241(-)APGAAQELQAKLAEIGAPIQGNR(E)
2362.21482362.1852137477671(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 13% (120/872AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/58 matches (13%).
Acc. #: Species: HUMAN Name: ATP-dependent RNA helicase DDX24 (DEAD-box protein 24)
Index: MW: 96333 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1350.71031350.6865181041161(K) (E)
1936.05391936.0656-6.1pyroGlu 5025190(R)QTLVFSATLTLVHQAPAR(I)
1937.05351936.9811371Met-ox 6096240(K)VLDIMPLTLHACMHQK(Q)
1953.03821952.9760322Met-ox 6096240(K)VLDIMPLTLHACMHQK(Q)
1953.03821953.0922-285025190(R) (I)
1967.04051967.0941-272442600(K) (R)
1969.02721969.1023-381892051(K) (A)
1982.99501983.0890-471Met-ox 2442600(K)TLAFAIPMIHAVLQWQK(R)
2362.21482362.1084455565751(R) (D)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 11% (101/859AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/58 matches (10%).
Acc. #: Species: HUMAN Name: Cullin homolog 4B (CUL-4B)
Index: MW: 84018 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1364.75431364.6963432132230(R) (R)
1566.77221566.7209331Met-ox 3813931(K)EATDEELEKMLDK(I)
1937.05351936.98163766801(R) (E)
2513.27652513.25737.71171370(R) (A)
2529.33722529.2522341Met-ox 1171370(R)TYVLQNSMLPSIWDMGLELFR(A)
2545.30442545.2471232Met-ox 1171370(R)TYVLQNSMLPSIWDMGLELFR(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (60/717AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 5/58 matches (8%).
Acc. #: Species: HUMAN Name: Coatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP)
Index: MW: 97623 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1566.77221566.75958.13944060(K) (D)
1929.99391929.98434.94164320(R) (E)
1937.05351937.015320941110(K) (D)
1953.03821953.0102141Met-ox 941110(K)EMATISEDVIIVTSSLTK(D)
2384.13572384.1994-275395600(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (70/871AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 4/58 matches (6%).
Acc. #: Species: HUMAN Name: Prostaglandin G/H synthase 1 precursor (Cyclooxygenase -1) (COX-1) (Prostaglandin-endoperoxide synthase 1) (Prostaglandin H2 synthase 1) (PGH synthase 1) (PGHS-1) (PHS 1)
Index: MW: 68657 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1936.05391935.9677452222390(K) (Q)
1982.99501982.9307325815990(R) (-)
2513.27652513.1899343763950(R) (V)
2545.30442545.1797492Met-ox 3763950(R)IAMEFNHLYHWHPLMPDSFK(V)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 9% (57/599AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 5/58 matches (8%).
Acc. #: Species: MOUSE Name: Histidine ammonia-lyase (Histidase)
Index: MW: 72259 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1922.06121921.9997323433591(K) (G)
1929.99391929.9055463703860(R) (F)
1941.00101941.0446-224524690(K) (R)
1952.00331951.947529pyroGlu 2322480(K)QVIEAFNASCLSYVPEK(G)
1969.02721968.9741272322480(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 10% (69/657AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 7/58 matches (12%).
Acc. #: Species: HUMAN Name: Thyroid receptor interacting protein 11 (TRIP-11)
Index: MW: 21952 Da pI: 9.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1129.61071129.60069.01671760(R) (S)
1179.60251179.56213481910(R) (S)
1264.69081264.65712717281(K) (V)
1280.67741280.6520201Met-ox 17281(K)KLMSLANSSEGK(V)
1475.76911475.7164365161(R) (K)
1707.83281707.8964-371311451(K) (K)
1952.00331952.0646-311771970(R) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 41% (81/197AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/58 matches (12%).
Acc. #: Species: MOUSE Name: Dual specificity mitogen-activated protein kinase kinase 4 (MAP kinase kinase 4) (MAPKK 4) (MAPK/ERK kinase 4) (JNK activating kinase 1) (C-JUN N-terminal kinase kinase 1) (JNK kinase 1) (JNKK 1) (SAPK/ERK kinase 1) (SEK1)
Index: MW: 44114 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1283.69391283.7323-302272370(R) (S)
1350.71031350.6516431Met-ox 3623710(K)HPFILMYEER(T)
1364.75431364.6918462Met-ox 1421520(K)QLLMDLDVVMR(S)
1929.99391929.97848.03333480(R) (D)
1952.00331952.0268-121892050(K) (I)
1994.08191994.0381222432601(K) (D)
2513.27652513.3080-132863071(R) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (105/397AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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