MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D63
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 1615 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/72(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.548e+00511 (15)74.015.353.942.9 18491/5.2HUMANATP synthase D chain, mitochondrial
25.310e+00510 (13)21.013.954.243.3 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
35.959e+00411 (15)15.015.311.769.8 93393/9.3MOUSEGeneral control of amino acid synthesis protein 5-like 2 (Histone acetyltransferase GCN5) (mmGCN5)
45.176e+0048 (11)18.011.13.74117 59348/9.0HUMANCytochrome P450 4A11 precursor (CYPIVA11) (Fatty acid omega-hydroxylase) (P-450 HK omega) (Lauric acid omega-hydroxylase) (CYP4AII) (P450-HL-omega)
52.817e+00410 (13)18.013.929.887.5 82806/9.1HUMANPoly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
62.345e+0048 (11)21.011.143.255.1 59556/5.4MOUSET-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
71.586e+00410 (13)13.013.97.4966.5 93837/9.1HUMANGeneral control of amino acid synthesis protein 5-like 2 (Histone acetyltransferase GCN5) (hsGCN5)
889399 (12)13.012.547.8111 87299/9.4MOUSEPlakophilin 3
976798 (11)17.011.134.283.9 68774/7.3MOUSEInterferon-gamma inducible protein MG11
1065867 (9)32.09.724.259.1 27978/5.9MOUSEDEAD-box RNA helicase DEAD2 (mDEAD2) (D1PAS1 related sequence 1)
66399...x..x...x....x......x...........x.x.x.............x..x............x...
45313...........xx.x.....x....x..x....x...x......................x....x......
47703.x...x.xx...x...x.....x.x...........x...................x..x............
43314......x.x.................x...x....x..........x........x..........x.....
108537....x......x.xx...x.....x...............x.....................x..xx.....
84429..........x..........x..x.......x...............xx...x...........x......
34694.x...x.xx.......x.....x.x...........x...................x..x............
86951.x.....x...x............x................x.........x...x.x....x.........
65301.........x.........x.........x......x....x....x..............x.........x
18186......x.........x...x.x...................x............xx...............


Detailed Results

1. 11/72 matches (15%).
Acc. #: Species: HUMAN Name: ATP synthase D chain, mitochondrial
Index: MW: 18491 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
993.5162993.48932764720(K) (K)
1093.56821093.52783733410(K) (L)
1121.60571121.58431964731(K) (F)
1180.63161180.5672541001090(K) (A)
1313.68831313.6299441Met-ox 1121211(R)IVEYEKEMEK(M)
1556.84251556.7538571501610(K) (-)
1624.86531624.77065886991(K) (S)
1684.95121684.8488611491611(K) (-)
1873.11861872.98607110250(K) (A)
1932.12431931.99076942580(R) (A)
2481.42182481.1859951Met-ox 1241440(K)NLIPFDQMTIEDLNEAFPETK(L)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 74% (120/161AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/72 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1125.56021125.5428153673760(K) (Y)
1127.58771127.5407422892981(K) (V)
1179.64121179.6010343773860(K) (H)
1300.62941300.5302762582670(K) (N)
1383.75971383.6909501861970(K) (V)
1475.84391475.7494642122230(K) (T)
1475.84391475.7858392002110(R) (W)
1523.88641523.7892644614721(R) (L)
1638.96621638.8604651861991(K) (F)
1994.08211993.9772532242390(R) (R)
2384.17672383.9524945195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 21% (137/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/72 matches (15%).
Acc. #: Species: MOUSE Name: General control of amino acid synthesis protein 5-like 2 (Histone acetyltransferase GCN5) (mmGCN5)
Index: MW: 93393 Da pI: 9.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
944.5419944.4954497777831(R) (K)
1090.56521090.56133.6pyroGlu 1911980(K)QVYFYLFK(L)
1107.57461107.5879-121911980(K) (L)
1109.54821109.53809.23063140(R) (S)
1127.58771127.5221582752840(R) (V)
1194.64551194.6846-336957050(R) (E)
1313.68831313.7469-456526620(R) (K)
1350.76281350.7091407647741(R) (L)
1350.76281350.7422155305401(K) (H)
1624.86531624.9063-254905040(R) (A)
1941.08221940.9805525825971(K) (H)
1963.05721963.0033272Met-ox 1671830(R)LLGMVVDVENLFMSVHK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 15% (126/830AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/72 matches (11%).
Acc. #: Species: HUMAN Name: Cytochrome P450 4A11 precursor (CYPIVA11) (Fatty acid omega-hydroxylase) (P-450 HK omega) (Lauric acid omega-hydroxylase) (CYP4AII) (P450-HL-omega)
Index: MW: 59348 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1093.56821093.5199443003090(K) (D)
1109.54821109.5149301Met-ox 3003090(K)MENGSILSDK(D)
1410.83711410.83610.712882990(R) (M)
1493.81461493.7270591Met-ox 3003121(K)MENGSILSDKDLR(A)
1566.83681566.7772384564681(R) (V)
1566.83681566.9372-642872991(K) (M)
1791.85361791.9944-794084230(K) (V)
1932.12431931.9874712552700(R) (K)
2389.38352389.5413-6611330(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 18% (94/519AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/72 matches (13%).
Acc. #: Species: HUMAN Name: Poly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
Index: MW: 82806 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.57711045.5464294694770(R) (E)
1125.56021125.55405.56466560(R) (R)
1140.64541140.64173.26646721(K) (D)
1179.64121179.55347573820(K) (N)
1232.65991232.6679-6.53603690(K) (Y)
1350.76281350.6324973140(K) (Q)
1700.93641700.9488-7.3pyroGlu 4915041(K)QLHHYLPAEILQKK(K)
2210.24422210.197421831031(K) (L)
2384.17672384.2145-161Met-ox 6186390(R)IITPHNPAQGQPHLNGMSNITK(T)
2389.38352389.14989841600(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (138/736AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/72 matches (11%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1121.60571121.59559.175840(R) (M)
1307.73301307.63847221310(K) (N)
1350.76281350.75684.53083181(K) (W)
1515.88871515.8131501531651(K) (T)
1839.06351838.9574581211370(R) (K)
1852.04891851.90237938541(K) (M)
1895.09631895.0101452072240(K) (K)
2384.17672384.1114272612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (118/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 10/72 matches (13%).
Acc. #: Species: HUMAN Name: General control of amino acid synthesis protein 5-like 2 (Histone acetyltransferase GCN5) (hsGCN5)
Index: MW: 93837 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
944.5419944.4954497847901(R) (K)
1090.56521090.56133.6pyroGlu 1962030(K)QVYFYLFK(L)
1107.57461107.5879-121962030(K) (L)
1109.54821109.53809.23113190(R) (S)
1194.64551194.6846-337027120(R) (E)
1313.68831313.7469-456596690(R) (K)
1350.76281350.7091407717811(R) (L)
1350.76281350.7422155375471(K) (H)
1624.86531624.9063-254975110(R) (A)
1941.08221940.9805525896041(K) (H)
1963.05721963.0033272Met-ox 1721880(R)LLGMVVDVENLFMSVHK(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (116/837AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 9/72 matches (12%).
Acc. #: Species: MOUSE Name: Plakophilin 3
Index: MW: 87299 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
944.5419944.5430-1.16416481(R) (L)
1107.57461107.4918757567651(R) (A)
1125.56021125.6493-793563651(K) (L)
1350.76281350.6905541741850(R) (L)
1706.94711706.8206745195321(K) (N)
1871.05421870.9187721952110(R) (A)
1932.12431931.9398953803961(R) (L)
1948.11211947.9347911Met-ox 3803961(R)HATGAMRNLIYDNVDNK(L)
2210.24422210.1358491031220(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 13% (108/797AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/72 matches (11%).
Acc. #: Species: MOUSE Name: Interferon-gamma inducible protein MG11
Index: MW: 68774 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1114.63171114.55407012201(K) (T)
1283.72841283.6207845295381(R) (Q)
1479.85561479.74847274860(K) (L)
1624.86531624.85933.71Met-ox 1141(-)MQSAPLEQPAKRPR(C)
1706.94711706.94034.03904040(K) (A)
1791.85361791.9216-381661810(R) (A)
2199.23012199.1524352712891(K) (G)
2807.52902807.41034274981(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 17% (105/594AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/72 matches (9%).
Acc. #: Species: MOUSE Name: DEAD-box RNA helicase DEAD2 (mDEAD2) (D1PAS1 related sequence 1)
Index: MW: 27978 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1093.56821093.55301460680(K) (M)
1194.64551194.64173.233430(R) (L)
1300.62941300.6398-8.01962051(K) (S)
1313.68831313.6022661Met-ox 941040(R)HTMMFSATFPK(E)
1313.68831313.6887-0.3080901(R) (G)
1707.91071707.89648.329431(R) (L)
1932.12431932.01255812280(R) (D)
1941.08221940.97925353681(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 32% (80/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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