MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D89
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (0 - 100000 Da) selects 107150 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12217 entries.
Pre searches select 12217 entries.

Data Set 1 Results

MS-Fit search selects 114 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/26(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
17.705e+00814 (53)65.053.877.526.4 29804/5.6HUMANProhibitin
21.489e+00813 (50)61.050.077.327.5 29820/5.6MOUSEPROHIBITIN (B-CELL RECEPTOR ASSOCIATED PROTEIN 32) (BAP 32)
314875 (19)8.019.240.379.5 84727/7.3MOUSEComplement C2 precursor (C3/C5 convertase)
43395 (19)10.019.254.8153 65871/8.4HUMANKeratin, type II cytoskeletal 2 oral (Cytokeratin 2P) (K2P) (CK 2P)
52015 (19)9.019.278.9162 64512/6.1HUMANKeratin, type II cytoskeletal 3 (Cytokeratin 3) (K3) (CK3) (65 kDa cytokeratin)
61974 (15)12.015.458.4120 34096/8.4HUMANCell division control protein 2 homolog (P34 protein kinase) (Cyclin-dependent kinase 1) (CDK1)
71365 (19)11.019.252.3130 48487/5.5HUMANKeratin, type I cytoskeletal 20 (Cytokeratin 20) (K20) (CK 20)
81315 (19)16.019.276.179.2 49111/8.9MOUSETubulin tyrosine ligase-like protein 1
91114 (15)9.015.467.0180 72730/5.5MOUSEGlutamate--cysteine ligase catalytic subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS heavy chain)
101004 (15)9.015.486.660.9 67197/6.7HUMANMitogen-activated protein kinase kinase kinase 7 (Transforming growth factor-beta-activated kinase 1) (TGF-beta-activated kinase 1)
70444.xxxxx.xx...x.x..x.xx..x.x
102060.xxxxx.xx...x....x.xx..x.x
49131x...x..............x.x..x.
30872..x......xxx............x.
1244..xx....x....x..........x.
50125x...x..x...........x......
25143...xx....x....x...x.......
61526...x.....x......x.x.x.....
10260..x..x...............xx...
108838...............xx.....xx..


Detailed Results

1. 14/26 matches (53%).
Acc. #: Species: HUMAN Name: Prohibitin
Index: MW: 29804 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1041.56501041.496566pyroGlu 1871950(K)QVAQQEAER(A)
1045.57761045.480293pyroGlu 1491570(R)QVSDDLTER(A)
1058.59591058.5231691871950(K) (A)
1062.58511062.5067741491570(R) (A)
1149.66751149.5904671341430(R) (E)
1185.74421185.65927284930(K) (I)
1213.81031213.7408571181280(R) (S)
1396.94921396.842976941050(R) (I)
1444.77481444.6596801061170(R) (V)
1606.97591606.8441822402531(R) (S)
1998.24321998.0871782202390(K) (K)
2119.29792119.1439731581770(R) (E)
2371.46122371.25238812350(K) (A)
3138.00753137.661011044700(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 65% (178/272AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/26 matches (50%).
Acc. #: Species: MOUSE Name: PROHIBITIN (B-CELL RECEPTOR ASSOCIATED PROTEIN 32) (BAP 32)
Index: MW: 29820 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1041.56501041.496566pyroGlu 1871950(K)QVAQQEAER(A)
1045.57761045.480293pyroGlu 1491570(R)QVSDDLTER(A)
1058.59591058.5231691871950(K) (A)
1062.58511062.5067741491570(R) (A)
1149.66751149.5904671341430(R) (E)
1185.74421185.65927284930(K) (I)
1213.81031213.7408571181280(R) (S)
1396.94921396.842976941050(R) (I)
1606.97591606.8441822402531(R) (S)
1998.24321998.0871782202390(K) (K)
2119.29792119.1439731581770(R) (E)
2371.46122371.25238812350(K) (A)
3138.00753137.661011044700(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 61% (166/272AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/26 matches (19%).
Acc. #: Species: MOUSE Name: Complement C2 precursor (C3/C5 convertase)
Index: MW: 84727 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
934.5307934.4668682832900(K) (I)
1062.58511062.5618222822901(K) (I)
1998.24321998.0700875415580(K) (R)
2211.29422211.2065402913100(R) (T)
2384.14672384.1823-155826020(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (68/760AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/26 matches (19%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 oral (Cytokeratin 2P) (K2P) (CK 2P)
Index: MW: 65871 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.57761045.5642133803890(K) (H)
1315.80341315.62831335245380(R) (G)
1356.97401356.78511394474581(K) (D)
1380.72681380.7011193083190(K) (T)
2384.14672384.2172-304674861(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (69/638AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/26 matches (19%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 3 (Cytokeratin 3) (K3) (CK3) (65 kDa cytokeratin)
Index: MW: 64512 Da pI: 6.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.57761045.5642133974060(K) (H)
1058.59591058.44651411Met-ox 3103180(K)DVDSAYMNK(V)
1213.81031213.64281381851950(K) (A)
1418.94041418.75311323253360(K) (T)
2384.14672384.2172-304845031(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (62/629AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 4/26 matches (15%).
Acc. #: Species: HUMAN Name: Cell division control protein 2 homolog (P34 protein kinase) (Cyclin-dependent kinase 1) (CDK1)
Index: MW: 34096 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
934.5307934.50322925330(K) (K)
1062.58511062.5981-1225341(K) (I)
1185.74421185.615610910200(K) (G)
1998.24321998.02711081071220(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (37/297AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 5/26 matches (19%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 20 (Cytokeratin 20) (K20) (CK 20)
Index: MW: 48487 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1058.59591058.5846113723801(R) (T)
1062.58511062.5948-9.11501581(K) (Y)
1315.80341315.63821263083180(K) (A)
1444.77481444.7535151881990(K) (D)
1877.18411876.95911201411561(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (50/424AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 5/26 matches (19%).
Acc. #: Species: MOUSE Name: Tubulin tyrosine ligase-like protein 1
Index: MW: 49111 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1058.59591058.5305622953040(K) (H)
1315.80341315.66551052162251(K) (Y)
1475.87031475.8195343954071(R) (A)
1877.18411876.94131291301461(K) (G)
2119.29792119.1916501761941(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (69/423AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 4/26 matches (15%).
Acc. #: Species: MOUSE Name: Glutamate--cysteine ligase catalytic subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS heavy chain)
Index: MW: 72730 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.57761045.6298-504574650(K) (V)
1149.66751149.52101274935030(K) (A)
2211.29422211.19854365841(K) (T)
2284.40272284.06681472883071(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (60/637AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 4/26 matches (15%).
Acc. #: Species: HUMAN Name: Mitogen-activated protein kinase kinase kinase 7 (Transforming growth factor-beta-activated kinase 1) (TGF-beta-activated kinase 1)
Index: MW: 67197 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1458.80681458.730153pyroGlu 3593721(K)QQSESGRLSLGASR(G)
1475.87031475.7566773593721(K) (G)
2284.40272284.1937914154360(K) (R)
2371.46122371.16381253884091(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (58/606AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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