MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D97
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (0 - 100000 Da) selects 107150 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12217 entries.
Pre searches select 12217 entries.

Data Set 1 Results

MS-Fit search selects 2553 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/87(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.547e+01017 (19)35.019.595.164.7 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
24.240e+00917 (19)53.019.587.462.2 29506/5.7HUMANProteasome activator complex subunit 3 (Proteasome activator 28-gamma subunit) (PA28gamma) (PA28g) (Activator of multicatalytic protease subunit 3) (11S regulator complex gamma subunit) (REG-gamma) (Ki nuclear autoantigen)
34.190e+00610 (11)28.011.596.856.6 35329/8.4HUMAN3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor (HIBADH)
41.415e+00613 (14)31.014.978.1102 59621/5.4HUMANT-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
57.197e+00512 (13)20.013.899.753.6 80509/5.5HUMANAdseverin (Scinderin)
64.077e+00515 (17)19.017.259.892.8 98146/7.0MOUSEDynamin 2 (Dynamin UDNM)
72.776e+00511 (12)22.012.697.536.9 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
81.159e+0058 (9)15.09.277.592.0 80642/6.6HUMANProtein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
96.492e+00411 (12)23.012.660.391.9 80625/7.3HUMANProtein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
104.459e+00410 (11)14.011.586.3122 89615/8.6HUMANTumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
45313.....x....x.....x...xx....xx.xx.....x...x.......x.....x...x...x.........x..........x...
60685...xx......x..x......x.x....x.........xx.x....x....x.x...x.............x..x..x.........
8076........................x.....x....x........x...........x....x.x.....xx..x.............
61980..x.........xx........x......x.x..x.........x...........x...........x........x....xx...
10776.............x....x........x..............x.........x........x.....x....xx.x.xx........
26696..x.x..x....x..........xx.x.....xx.x...................x.....xx.......x...........x....
97443.xx...x........................x.xx..............................x.xx..x.............x.
84524............x..x........................xx...x............x........................x..x
7149x.........x.....x.......xx.....x..........x.....x.........................x.......x..x.
42717...x............x.....................x.x.x...............x...x..x..x...x..............


Detailed Results

1. 17/87 matches (19%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1092.65671092.50341406036160(R) (G)
1141.66251141.51991254644720(R) (L)
1179.71641179.6010983773860(K) (H)
1217.60701217.6166-7.96346440(R) (-)
1231.74021231.6283913563661(K) (A)
1265.75551265.6378932782880(R) (K)
1277.84611277.71051064734830(K) (T)
1300.67741300.53021132582670(K) (N)
1302.78431302.7018633934031(R) (V)
1302.78431302.7157533443550(R) (A)
1383.82891383.69091001861970(K) (V)
1475.90051475.74941022122230(K) (T)
1475.90051475.7858782002110(R) (W)
1639.00661638.8604891861991(K) (F)
1688.15921687.970711266821(R) (G)
1700.00401699.8251105pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1717.05541716.85171194184320(K) (G)
1994.19991993.97721122242390(R) (R)
2384.22722383.95241155195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (227/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 17/87 matches (19%).
Acc. #: Species: HUMAN Name: Proteasome activator complex subunit 3 (Proteasome activator 28-gamma subunit) (PA28gamma) (PA28g) (Activator of multicatalytic protease subunit 3) (11S regulator complex gamma subunit) (REG-gamma) (Ki nuclear autoantigen)
Index: MW: 29506 Da pI: 5.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1078.60101078.4958981962030(K) (R)
1090.59621090.5744202042121(R) (E)
1155.78021155.6712941381460(K) (I)
1171.76551171.6662851Met-ox 1381460(K)MWVQLLIPR(I)
1231.74021231.7084261281371(R) (M)
1234.72611234.59691051962041(K) (T)
1286.81121286.6956901111210(K) (V)
1440.80301440.6317119891000(R) (V)
1474.90181474.7728872262370(R) (N)
1490.91391490.7677981Met-ox 2262370(R)NQYVTLHDMILK(N)
1596.90461596.7328108881001(R) (V)
1668.99031668.80811091671810(R) (Y)
1681.01581680.848510022360(R) (K)
1696.06281695.88051082052181(R) (L)
1966.18311965.99229720361(R) (K)
2009.19912009.1395301111271(K) (L)
2220.33162220.07841141471660(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 53% (136/254AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/87 matches (11%).
Acc. #: Species: HUMAN Name: 3-hydroxyisobutyrate dehydrogenase, mitochondrial precursor (HIBADH)
Index: MW: 35329 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1245.74531245.6268953223310(K) (E)
1302.78431302.7568212392501(K) (C)
1373.85441373.7218973213311(K) (E)
1568.06201567.89601062983110(K) (M)
1693.01911692.82021171501670(K) (S)
1708.96701708.8151891Met-ox 1501670(K)MGAVFMDAPVSGGVGAAR(S)
1724.99041724.81001052Met-ox 1501670(K)MGAVFMDAPVSGGVGAAR(S)
1881.09191880.87071183223361(K) (-)
1922.17521921.952211661760(K) (E)
2006.14462005.935510477950(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (95/336AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/87 matches (14%).
Acc. #: Species: HUMAN Name: T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta)
Index: MW: 59621 Da pI: 5.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.61201065.5217853914000(R) (V)
1158.70331158.5907974674760(K) (N)
1168.68191168.6002701721810(K) (L)
1232.73771232.60981042973070(K) (Y)
1300.67741300.7411-493243351(R) (L)
1307.80981307.638413121310(K) (N)
1350.82101350.7568483083181(K) (W)
1568.06201567.84441393653781(K) (G)
1693.01911692.834610917311(K) (N)
1852.14691851.902313238541(K) (M)
2220.33162220.1885642042241(R) (K)
2298.35092298.26443855741(K) (E)
2384.22722384.1114492612811(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (172/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 12/87 matches (13%).
Acc. #: Species: HUMAN Name: Adseverin (Scinderin)
Index: MW: 80509 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1168.68191168.53081296766850(K) (D)
1193.72701193.55911414120(R) (A)
1277.84611277.7469783593691(K) (Q)
1493.89881493.72761151501620(R) (G)
1678.93071678.76031012Met-ox 2482621(K)MAKLYMVSDASGSMR(V)
1708.96701708.8758534484620(R) (S)
1838.14291837.9772901191370(K) (R)
1994.19991994.0783611191381(K) (L)
2006.14462005.93631041Met-ox 3083241(K)AAMKTAEEFLQQMNYSK(N)
2097.17922096.9889915986150(K) (L)
2220.33162220.09371075455641(K) (G)
2225.36622225.08391275976151(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (149/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 15/87 matches (17%).
Acc. #: Species: MOUSE Name: Dynamin 2 (Dynamin UDNM)
Index: MW: 98146 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.61201065.6421-281141230(K) (V)
1090.59621090.501787991070(R) (V)
1107.67361107.57988545540(K) (D)
1158.70331158.6734263003090(K) (E)
1234.72611234.6870325625711(K) (I)
1245.74531245.6340893163251(K) (T)
1265.75551265.6795602572661(R) (H)
1309.80761309.7844182182281(R) (R)
1323.78951323.6367115pyroGlu 4414511(R)QCTSKLSSYPR(L)
1373.85441373.8004392983091(R) (E)
1691.01211690.8248111991131(R) (G)
1708.96701708.9723-3.17707850(R) (G)
1717.05541716.87771031Met-ox 1151(-)MGNRGMEELIPLVNK(L)
1922.17521921.93761245405540(K) (D)
2298.35092298.2610393944141(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (166/870AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 11/87 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1060.64711060.5639782242320(K) (M)
1065.61201065.49991051541620(K) (L)
1095.64061095.5475851841911(K) (G)
1307.80981307.67821012402491(R) (Q)
1323.78951323.6731881Met-ox 2402491(R)IKFEMEQNLR(Q)
1350.82101350.6878994724861(K) (G)
1791.93581791.72831164905120(R) (G)
1838.14291837.9660963743890(R) (K)
1852.14691851.92751183213351(K) (N)
1966.18311966.0609623743901(R) (A)
2705.49642705.161712463940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (138/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/87 matches (9%).
Acc. #: Species: HUMAN Name: Protein-tyrosine phosphatase epsilon precursor (R-PTP-epsilon)
Index: MW: 80642 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1158.70331158.6557416066160(K) (Q)
1174.77161174.65061031Met-ox 6066160(K)GMIDLIAAVQK(Q)
1475.90051475.70991294414531(K) (K)
1475.90051475.8256512272391(K) (K)
1490.91391490.71361342772880(K) (A)
1571.91661571.748810786980(K) (V)
1700.00401699.84379485981(K) (V)
2384.22722384.1531316676840(R) (V)
2717.43462717.41656.62923150(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (105/700AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 11/87 matches (12%).
Acc. #: Species: HUMAN Name: Protein-glutamine gamma-glutamyltransferase 3-like (Transglutaminase 3-like) (TGase 3-like)
Index: MW: 80625 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.65331045.54641023823910(R) (E)
1141.66251141.6734-9.57097190(K) (-)
1179.71641179.60109885940(K) (G)
1245.74531245.6989375265360(R) (A)
1262.74651262.7216201Met-ox 5956050(K)ILLAAMCLVTK(G)
1307.80981307.7099766146240(K) (V)
1307.80981307.7323595425530(R) (K)
1493.89881493.80766147591(R) (A)
1639.00661638.83391054414551(K) (Y)
2009.19912008.972411360760(R) (A)
2298.35092298.1532862452651(R) (G)
2705.49642705.5639-251Met-ox 6256501(K)VLGPAMVGVAVTVEVTVVNPLIERVK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (168/719AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 10/87 matches (11%).
Acc. #: Species: HUMAN Name: Tumor necrosis factor, alpha-induced protein 3 (Putative DNA binding protein A20) (Zinc finger protein A20)
Index: MW: 89615 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1078.60101078.48521075735810(R) (A)
1179.71641179.55811346776850(K) (F)
1440.80301440.8725-482052161(R) (S)
1475.90051475.68411471Met-ox 2882990(K)VHFLTDPENEMK(E)
1493.89881493.69211386226320(K) (E)
1700.00401699.93434167811(K) (K)
1717.05541716.82671332883011(K) (L)
1791.93581791.8601426776901(K) (R)
1852.14691851.9751934214390(K) (G)
1994.19991994.05007525410(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (111/790AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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