MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D98
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (0 - 100000 Da) selects 107150 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12217 entries.
Pre searches select 12217 entries.

Data Set 1 Results

MS-Fit search selects 1956 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/90(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.652e+00615 (16)53.016.7-92.126.8 35883/5.8HUMANAnnexin A4 (Annexin IV) (Lipocortin IV) (Endonexin I) (Chromobindin 4) (Protein II) (P32.5) (Placental anticoagulant protein II) (PAP-II) (PP4-X) (35-beta calcimedin) (Carbohydrate-binding protein P33/P41) (P33/41)
22.001e+0059 (10)22.010.0-66.9104 57652/9.6HUMANZinc finger protein 169
31.529e+00512 (13)22.013.3-68.5104 86984/6.5HUMANCullin homolog 2 (CUL-2)
41.184e+0059 (10)15.010.0-72.190.2 82433/6.8HUMANATP-dependent helicase DDX1 (DEAD-box protein 1) (DEAD-box protein-retinoblastoma) (DBP-RB)
58.147e+0048 (8)12.08.9-78.4114 96743/9.0HUMANVoltage-gated potassium channel protein KQT-like 3
66.411e+0048 (8)13.08.9-82.655.5 99916/6.4MOUSEVav-2 protein
73.688e+0049 (10)23.010.0-89.869.5 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
82.327e+0047 (7)14.07.8-78.787.6 78092/9.2HUMANRING finger protein 6
92.316e+00410 (11)16.011.1-65.491.6 80509/5.5HUMANAdseverin (Scinderin)
102.313e+00411 (12)19.012.2-87.8104 71175/7.0HUMANFragile X mental retardation 1 protein (Protein FMR-1) (FMRP)
84672....x.....xx.x...........xx.......x..xx..x.x.x.................x..............x..........x
87949........x.....xx............x...x......................x................x.....x...x.......
51616...............x..x......x.........x...xx....x....x............x.......x..........x.....x.
310...............x.x.....................x...x.....x......xxx..............x................
72811.........x.......................x..........x................x............x.x..x.x........
13019.....................x....x.............x........x.............x.x.x...x..................
45313x...........x..x..x............x...x........x.....................x...............x.......
98309...................x........x..........................x.......xx........xx...............
10776....x....................x...x..xx.....................x..............xx...x..x...........
53743.....x.........x..x..x.....x.......xx.............x...............x.x...................x.


Detailed Results

1. 15/90 matches (16%).
Acc. #: Species: HUMAN Name: Annexin A4 (Annexin IV) (Lipocortin IV) (Endonexin I) (Chromobindin 4) (Protein II) (P32.5) (Placental anticoagulant protein II) (PAP-II) (PP4-X) (35-beta calcimedin) (Carbohydrate-binding protein P33/P41) (P33/41)
Index: MW: 35883 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1009.45521009.5505-941932000(K) (N)
1091.42941091.5407-1021Met-ox 2762840(R)AEIDMLDIR(A)
1118.40571118.4941-791421500(R) (V)
1134.37081134.4890-1041Met-ox 1421500(R)SDTSFMFQR(V)
1371.53771371.6657-931241340(R) (S)
1379.51721379.6443-921611720(R) (Q)
1527.62111527.7668-951231341(R) (S)
1570.66091570.8130-972032141(R) (I)
1597.58801597.7280-881Met-ox 10240(K)AASGFNAMEDAQTLR(K)
1666.67791666.8540-1062262410(K) (C)
1675.69391675.8325-831011160(K) (T)
1692.72271692.8808-9329440(K) (N)
1962.77251962.8867-581Met-ox 1351501(R)SLEDDIRSDTSFMFQR(V)
2319.00332319.1945-821511721(R) (Q)
3243.22993243.6030-1152Met-ox 70971(K)SELSGNFEQVIVGMMTPTVLYDVQELRR(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 53% (170/319AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/90 matches (10%).
Acc. #: Species: HUMAN Name: Zinc finger protein 169
Index: MW: 57652 Da pI: 9.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1050.46641050.5220-532112190(R) (R)
1171.41621171.5339-1002Met-ox 1021120(R)MSLGGSDTMLK(G)
1179.49281179.5152-191431510(K) (G)
1420.61861420.6192-0.4151640(K) (K)
1493.59651493.7753-1205180(K) (Q)
1847.76061847.7839-134444580(R) (G)
2198.85612199.1484-1332492670(R) (A)
2319.00332319.1039-434164351(R) (S)
2383.77012384.0586-1214444631(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (116/512AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/90 matches (13%).
Acc. #: Species: HUMAN Name: Cullin homolog 2 (CUL-2)
Index: MW: 86984 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.49281179.4952-2.04054140(K) (L)
1277.54871277.529515971060(K) (Y)
1371.53771371.6401-752Met-ox 2923020(K)NDMANMYVLLR(A)
1542.66951542.8657-12763741(K) (R)
1610.73531610.7749-254634751(K) (M)
1661.72531661.8937-1013874001(K) (K)
1692.72271692.8995-1047217331(K) (S)
1731.79681731.8740-4576890(R) (Y)
1962.77251962.8721-51pyroGlu 7297451(K)QYIERSQASADEYSYVA(-)
1962.77251962.9172-745365510(K) (K)
2176.93332176.9821-226306481(K) (T)
2383.77012384.0903-1342Met-ox 4764951(R)MYTDMSVSADLNNKFNNFIK(N)
2716.89552717.2921-14676961(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (171/745AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/90 matches (10%).
Acc. #: Species: HUMAN Name: ATP-dependent helicase DDX1 (DEAD-box protein 1) (DEAD-box protein-retinoblastoma) (DBP-RB)
Index: MW: 82433 Da pI: 6.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.49281179.6196-1075685770(R) (G)
1232.50941232.6924-1496126230(R) (E)
1610.73531610.8073-453874001(R) (L)
1675.69391675.9019-1242973101(R) (K)
1713.68971713.7689-46991140(R) (E)
1851.76661851.7907-131331460(K) (V)
1879.77311879.9125-7483981(K) (G)
1895.80481895.9074-541Met-ox 83981(K)TGASVLNKWQMNPYDR(G)
2210.92142211.0028-375175350(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (115/740AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/90 matches (8%).
Acc. #: Species: HUMAN Name: Voltage-gated potassium channel protein KQT-like 3
Index: MW: 96743 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1090.40621090.5380-1214144230(K) (L)
1524.68861524.7527-424014120(R) (K)
1687.79531687.7175468148280(R) (E)
1929.76141929.9138-796356491(K) (L)
2220.88352221.1327-1125015201(R) (A)
2236.85162237.1276-1231Met-ox 5015201(R)GYGNDFPIEDMIPTLKAAIR(A)
2332.96032333.2155-1093733921(R) (I)
2368.86992369.0753-876016210(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (113/872AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 8/90 matches (8%).
Acc. #: Species: MOUSE Name: Vav-2 protein
Index: MW: 99916 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1301.48411301.5551-556126220(R) (L)
1379.51721379.6418-905365460(R) (C)
1661.72531661.8950-10210221(R) (V)
1713.68971713.8124-72901030(R) (D)
1962.77251962.8696-496556700(R) (Q)
1984.78091984.8971-594935080(K) (A)
2042.79392043.0372-1196796961(K) (F)
2176.93332177.1831-1153343511(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (117/868AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 9/90 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
910.3175910.4158-1082702760(K) (R)
1127.43391127.5407-952892981(K) (V)
1179.49281179.6010-923773860(K) (H)
1277.54871277.6337-672472571(K) (N)
1277.54871277.7105-1274734830(K) (T)
1475.60691475.7494-972122230(K) (T)
1475.60691475.7858-1212002110(R) (W)
1542.66951542.66810.912582691(K) (Y)
1687.79531687.9707-10466821(R) (G)
1993.77141993.9772-1032242390(R) (R)
2383.77012383.9524-765195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 23% (149/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: RING finger protein 6
Index: MW: 78092 Da pI: 9.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1292.53881292.7327-1503944040(R) (R)
1420.61861420.6821-452212340(R) (L)
1847.76061847.9364-952172341(R) (L)
1962.77251962.9197-751031180(R) (R)
1970.78911970.9949-1042752921(R) (N)
2210.92142210.9518-1437530(R) (L)
2220.88352221.0346-682482681(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (101/685AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 10/90 matches (11%).
Acc. #: Species: HUMAN Name: Adseverin (Scinderin)
Index: MW: 80509 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1009.45521009.4896-34pyroGlu 3423481(K)QFFKDWR(D)
1371.53771371.7133-1283984081(K) (I)
1427.68481427.6477261Met-ox 6766871(K)MYLETDPSGRDK(R)
1493.59651493.7276-881501620(R) (G)
1524.68861524.8116-8157681(R) (E)
1847.76061847.9656-1111471621(R) (G)
2096.77242096.9889-1035986150(K) (L)
2176.93332177.0092-356967140(K) (W)
2224.97752225.0839-485976151(K) (L)
2319.00332319.1257-535775961(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (116/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 11/90 matches (12%).
Acc. #: Species: HUMAN Name: Fragile X mental retardation 1 protein (Protein FMR-1) (FMRP)
Index: MW: 71175 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1018.40471018.5362-12949570(R) (D)
1179.49281179.5606-575946040(K) (L)
1277.54871277.5811-2575830(K) (V)
1301.48411301.5432-451Met-ox 4604710(K)SYVTDDGQGMGR(G)
1390.55081390.7364-1331962071(K) (Q)
1542.66951542.6858-114584711(K) (G)
1566.61241566.8143-1293944070(K) (D)
1731.79681731.8626-385896041(R) (L)
1993.77141994.0288-129pyroGlu 41571(R)QIPFHDVRFPPPVGYNK(D)
2082.76872083.0209-1213553721(K) (V)
2716.89552717.2960-14718401(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (123/632AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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