MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D101
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 210 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/56(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	3225	6 (10)	10.0	10.7	-24.9	117		47820/6.6		HUMAN	TYPE I INOSITOL-1,4,5-TRISPHOSPHATE 5-PHOSPHATASE (5PTASE)
2	1851	6 (10)	9.0	10.7	-59.1	58.8		77888/8.7		HUMAN	Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
3	642	5 (8)	9.0	8.9	-31.1	103		84879/5.9		MOUSE	Muskelin
4	535	6 (10)	13.0	10.7	8.67	101		45875/9.2		MOUSE	ZINC FINGER PROTEIN GFI-1 (GROWTH FACTOR INDEPENDENCE-1)
5	464	5 (8)	5.0	8.9	-7.43	153		85941/6.2		HUMAN	Signal transducer and activator of transcription 4

14161	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	x	x	.	.	.	.	x	.	.	.
47898	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.
4624	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	x
77869	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.	.	.	.
80593	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	x	x	.	.	.	.	.	.	.	.

Detailed Results

1. 6/56 matches (10%).
Acc. #: Species: HUMAN Name: TYPE I INOSITOL-1,4,5-TRISPHOSPHATE 5-PHOSPHATASE (5PTASE)
Index: MW: 47820 Da pI: 6.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1043.4533	1043.5050	-50		89	96	0	(R) (S)
1126.4168	1126.4951	-70		335	343	0	(R) (C)
1142.5042	1142.4900	12	1Met-ox	335	343	0	(R)QGEQYMNTR(C)
1280.4710	1280.5581	-68		60	70	0	(K) (F)
1296.6533	1296.5530	77	1Met-ox	60	70	0	(K)NYEASMSHVDK(F)
1940.8531	1940.9547	-52		225	240	1	(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (44/412AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/56 matches (10%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1008.3970 1008.4607 -63 225 231 1 (R) (L)
1056.4833 1056.5842 -95 271 279 0 (R) (C)
1163.5428 1163.6173 -64 342 351 0 (K) (H)
1651.6954 1651.8226 -77 338 351 1 (R) (H)
2210.9746 2211.0756 -46 143 161 0 (K) (T)
2225.0091 2225.0304 -9.6 606 624 0 (R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (68/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/56 matches (8%).
Acc. #: Species: MOUSE Name: Muskelin
Index: MW: 84879 Da pI: 5.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1057.3900 1057.4777 -83 556 563 0 (R) (N)
1073.5064 1073.5665 -56 648 657 0 (K) (Y)
1256.6480 1256.5806 54 320 330 1 (K) (M)
1373.6227 1373.6670 -32 1 14 0 (-) (L)
2770.2275 2770.3324 -38 23 46 0 (K) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (67/735AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/56 matches (10%).
Acc. #: Species: MOUSE Name: ZINC FINGER PROTEIN GFI-1 (GROWTH FACTOR INDEPENDENCE-1)
Index: MW: 45875 Da pI: 9.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1120.5733 1120.5672 5.4 240 248 0 (K) (L)
1123.5548 1123.5761 -19 11 19 1 (K) (S)
1142.5042 1142.5338 -26 312 320 1 (R) (S)
1353.7628 1353.6915 53 293 304 0 (K) (A)
1590.9885 1590.8525 85 235 248 1 (K) (L)
1651.6954 1651.7725 -47 391 404 0 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (58/423AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/56 matches (8%).
Acc. #: Species: HUMAN Name: Signal transducer and activator of transcription 4
Index: MW: 85941 Da pI: 6.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1051.6181 1051.5610 54 375 383 0 (R) (A)
1120.5733 1120.5427 27 622 630 0 (R) (G)
1166.4252 1166.5363 -95 177 186 0 (K) (N)
1280.4710 1280.5793 -85 152 162 0 (K) (Y)
1296.6533 1296.5742 61 1Met-ox 152 162 0 (K)NSVQMTEQDTK(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 5% (39/748AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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