MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D80
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1576 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/133(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	5.374e+004	6 (4)	22.0	4.5	-17.3	51.4		42723/6.5		MOUSE	MYG1 protein (Gamm1 protein)
2	4.707e+004	12 (9)	22.0	9.0	-18.6	51.3		68103/9.1		HUMAN	Zinc finger protein 44 (Zinc finger protein KOX7) (Gonadotropin inducible transcription repressor-2) (GIOT-2)
3	4.094e+004	9 (6)	14.0	6.8	-17.5	56.3		93559/7.8		HUMAN	Axin 2 (Axis inhibition protein 2) (Conductin) (Axin-like protein) (Axil)
4	3.117e+004	9 (6)	21.0	6.8	-20.0	50.0		50320/4.8		HUMAN	Keratin, type I cuticular HA2 (Hair keratin, type I HA2)
5	3.116e+004	8 (6)	16.0	6.0	-17.2	48.8		53565/9.0		HUMAN	Zinc finger protein 135

13540	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.
60258	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
14660	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.
70849	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
56867	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 6/133 matches (4%).
Acc. #: Species: MOUSE Name: MYG1 protein (Gamm1 protein)
Index: MW: 42723 Da pI: 6.5

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1114.5551	1114.5567	-1.4		188	197	0	(R) (V)
1419.7307	1419.7749	-31		128	140	0	(K) (K)
1478.6662	1478.7313	-44		219	230	0	(R) (L)
1581.7203	1581.7913	-45		273	285	0	(K) (V)
2113.0352	2113.0098	12		110	127	0	(R) (L)
2230.0688	2230.0563	5.6		84	103	0	(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (86/380AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/133 matches (9%).
Acc. #: Species: HUMAN Name: Zinc finger protein 44 (Zinc finger protein KOX7) (Gonadotropin inducible transcription repressor-2) (GIOT-2)
Index: MW: 68103 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
979.4666 979.4705 -4.0 1Met-ox 39 46 0 (R)NLNCIGMK(W)
1066.5720 1066.5468 24 27 34 1 (K) (E)
1123.5222 1123.5570 -31 65 73 1 (R) (S)
1153.5095 1153.5642 -47 401 410 0 (K) (H)
1158.5124 1158.5690 -49 62 70 1 (R) (F)
1318.5493 1318.5785 -22 383 394 0 (R) (C)
1378.5738 1378.5996 -19 2Met-ox 327 338 0 (R)HMIMHSGDGPHK(C)
1464.6493 1464.6753 -18 76 88 0 (K) (N)
1809.7551 1809.8268 -40 47 60 0 (K) (R)
1834.9501 1834.8944 30 302 316 1 (R) (A)
1850.8358 1850.8529 -9.3 414 428 1 (R) (A)
1855.8303 1855.9013 -38 354 368 0 (R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (134/589AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/133 matches (6%).
Acc. #: Species: HUMAN Name: Axin 2 (Axis inhibition protein 2) (Conductin) (Axin-like protein) (Axil)
Index: MW: 93559 Da pI: 7.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1114.5551 1114.5017 48 263 272 0 (R) (S)
1175.5113 1175.5544 -37 529 538 0 (K) (V)
1320.5723 1320.6265 -41 713 722 1 (K) (D)
1328.6138 1328.6195 -4.3 57 68 1 (R) (A)
1493.6254 1493.6695 -30 561 574 0 (K) (G)
1519.7103 1519.7505 -26 643 656 1 (K) (A)
1693.7461 1693.7644 -11 616 628 0 (R) (Q)
1791.8253 1791.9030 -43 81 96 0 (K) (T)
2550.1390 2550.1732 -13 104 123 1 (K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (119/843AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/133 matches (6%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA2 (Hair keratin, type I HA2)
Index: MW: 50320 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1094.4715 1094.5040 -30 398 406 0 (R) (L)
1153.5095 1153.5312 -19 98 106 0 (K) (L)
1169.5209 1169.5261 -4.5 1Met-ox 98 106 0 (K)ETMQFLNDR(L)
1169.5209 1169.5551 -29 233 242 0 (K) (C)
1464.6493 1464.6818 -22 334 346 0 (R) (Y)
1487.6924 1487.7494 -38 pyroGlu 375 386 0 (R)QNQEYQVLLDVR(A)
1504.7442 1504.7760 -21 375 386 0 (R) (A)
1540.6470 1540.7001 -34 270 281 1 (R) (D)
1791.8253 1791.9063 -45 167 182 1 (R) (A)
1857.9314 1857.8509 43 1Met-ox 1 16 0 (-)MTSSCCVTNNLQASLK(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (97/448AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/133 matches (6%).
Acc. #: Species: HUMAN Name: Zinc finger protein 135
Index: MW: 53565 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
977.4376 977.4441 -6.6 122 129 0 (R) (T)
1078.5202 1078.5533 -31 453 462 0 (K) (H)
1090.5158 1090.5394 -22 pyroGlu 428 436 0 (R)QSTHLTQHR(R)
1107.5149 1107.5659 -46 428 436 0 (R) (R)
1263.6119 1263.6670 -44 428 437 1 (R) (I)
1791.8253 1791.8158 5.3 158 172 0 (R) (A)
1809.7551 1809.7900 -19 270 284 0 (R) (A)
2282.1137 2282.0559 25 27 45 1 (K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (77/469AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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