MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D135
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 2765 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/104(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.066e+00814 (13)31.013.525.854.1 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
21.083e+0079 (8)42.08.727.539.7 35995/6.6HUMANDelta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
33.205e+00510 (9)15.09.66.7668.8 97116/6.2MOUSEEphrin type-A receptor 5 precursor (Tyrosine-protein kinase receptor EHK-1) (EPH homology kinase-1) (Brain-specific kinase) (CEK-7)
41.572e+00511 (10)21.010.612.556.9 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
51.190e+00510 (9)23.09.67.7674.5 65866/8.1HUMANKeratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
45313..x.........x.x..x....x..................x.......x...........xxx..x............x............x.x.........
93995............................x.............x....xx..........x.....................x..x...............xx..
2039.......x.....................x.............x.xxx.x..........x.........................x.........x.......
97443x....xx.....x..x..............x...x....x....................................xx.....................x....
110808..x......x......x...x........x...x..x............x..................................x.........x.........


Detailed Results

1. 14/104 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4972973.5318-363964030(K) (V)
1157.56471157.5149431Met-ox 4644720(R)DYQELMNTK(L)
1179.62281179.6010193773860(K) (H)
1217.58771217.6166-246346440(R) (-)
1277.74021277.7105234734830(K) (T)
1383.74601383.6909401861970(K) (V)
1475.80681475.7494392122230(K) (T)
1475.80681475.7858142002110(R) (W)
1638.92781638.8604411861991(K) (F)
1657.89081657.79345913290(R) (R)
1687.97311687.97071.466821(R) (G)
1716.91541716.8517374184320(K) (G)
1994.04711993.9772352242390(R) (R)
2300.29212300.1985413443641(R) (S)
2384.08172383.9524545195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (204/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/104 matches (8%).
Acc. #: Species: HUMAN Name: Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Index: MW: 35995 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1298.67431298.6381281491580(R) (C)
1390.67891390.6465231861960(R) (E)
1450.74351450.7443-0.571321431(K) (D)
1454.86391454.83442066770(K) (R)
1610.98551610.93563166781(K) (N)
2097.02212096.9997111Met-ox 2322501(K)MMADEALDSGLVSRVFPDK(E)
2097.02212097.00707.21131310(K) (G)
2145.07962144.9918413Met-ox 1131310(K)MFTAGIDLMDMASDILQPK(G)
2761.45872761.29176033590(R) (V)
2834.60152834.4479541591850(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (138/328AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/104 matches (9%).
Acc. #: Species: MOUSE Name: Ephrin type-A receptor 5 precursor (Tyrosine-protein kinase receptor EHK-1) (EPH homology kinase-1) (Brain-specific kinase) (CEK-7)
Index: MW: 97116 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1073.50061073.5115-104654731(K) (M)
1073.50061073.5487-458688770(K) (-)
1301.67541301.7251-386446540(R) (V)
1406.70741406.6408471381500(R) (E)
1412.69921412.7273-203483591(K) (E)
1434.80511434.74554270820(R) (N)
1450.74351450.74042.11Met-ox 70820(R)TVMGDLGWIAFPK(N)
1475.80681475.7681268088201(R) (Y)
1614.91701614.8274553013151(K) (G)
2209.16692209.1869-9.03603790(K) (A)
2565.26242565.2014247367560(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (137/877AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/104 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
913.3778913.4089-341Met-ox 2332390(R)MTLDDFR(I)
1060.58291060.5639182242320(K) (M)
1065.52971065.4999281541620(K) (L)
1157.56471157.5915-232502600(R) (Q)
1190.59801190.6091-9.32612700(R) (S)
1307.70821307.6782232402491(R) (Q)
1323.65721323.6731-121Met-ox 2402491(R)IKFEMEQNLR(Q)
1350.71491350.6878204724861(K) (G)
1838.02061837.9660303743890(R) (K)
1851.99861851.9275383213351(K) (N)
2705.32122705.16175963940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (134/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/104 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.4972973.5318-364004070(K) (V)
1107.56611107.5435203603680(K) (S)
1193.65531193.6166323813900(K) (H)
1193.65531193.6279232442531(K) (T)
1254.65641254.60793921340(R) (R)
1301.67541301.62723735451(R) (H)
1320.63921320.58344246610(R) (S)
1329.69961329.6399454254360(K) (G)
1329.69961329.7266-203483590(R) (A)
1475.80681475.7858142042150(R) (W)
2145.07962145.1807-473033201(K) (V)
2384.08172384.2172-574684871(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (152/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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