MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D141
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 254 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/42(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	3.862e+005	10 (23)	55.0	23.8	23.3	44.3		25035/6.0		HUMAN	Antioxidant protein 2 (1-Cys peroxiredoxin) (1-Cys PRX) (Acidic calcium-independent phospholipase A2) (aiPLA2) (Non-selenium glutathione peroxidase) (NSGPx) (24 kDa protein) (Liver 2D page spot 40) (Red blood cells page spot 12)
2	1.433e+004	6 (14)	32.0	14.3	28.5	33.8		28152/6.4		MOUSE	Hypothetical protein CGI-99 homolog
3	8419	5 (11)	28.0	11.9	25.1	29.9		28068/6.2		HUMAN	Hypothetical protein CGI-99
4	8012	6 (14)	14.0	14.3	0.959	87.4		53386/8.6		HUMAN	HYPOTHETICAL PROTEIN DJ845O24.1
5	7421	6 (14)	35.0	14.3	14.8	63.7		24871/5.7		MOUSE	Antioxidant protein 2 (1-Cys peroxiredoxin) (1-Cys PRX) (Acidic calcium-independent phospholipase A2) (aiPLA2) (Non-selenium glutathione peroxidase) (NSGPx)

19334	.	.	x	.	x	x	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	x	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.
48328	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
67613	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
18926	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	x	.	.	.	.	.	x
28252	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.

Detailed Results

1. 10/42 matches (23%).
Acc. #: Species: HUMAN Name: Antioxidant protein 2 (1-Cys peroxiredoxin) (1-Cys PRX) (Acidic calcium-independent phospholipase A2) (aiPLA2) (Non-selenium glutathione peroxidase) (NSGPx) (24 kDa protein) (Liver 2D page spot 40) (Red blood cells page spot 12)
Index: MW: 25035 Da pI: 6.0

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
906.4552	906.4685	-15		156	162	0	(R) (V)
1085.6114	1085.5995	11		98	106	0	(K) (N)
1135.6532	1135.6516	1.5		133	142	1	(R) (L)
1191.6885	1191.6737	12		145	155	0	(K) (N)
1356.8368	1356.8215	11		163	174	1	(R) (V)
1409.7133	1409.6735	28		42	53	0	(R) (A)
1596.7508	1596.6852	41		85	97	0	(K) (L)
1897.0974	1897.0071	48		68	84	0	(K) (D)
1901.0881	1900.9942	49	1Met-ox	109	125	1	(R)ELAILLGMLDPAEKDEK(G)
2031.0794	2030.9877	45		25	41	0	(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 55% (125/224AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/42 matches (14%).
Acc. #: Species: MOUSE Name: Hypothetical protein CGI-99 homolog
Index: MW: 28152 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1022.6018 1022.5999 1.9 206 213 0 (R) (E)
1297.7298 1297.6615 53 63 72 1 (K) (I)
1483.8683 1483.8273 28 77 89 0 (R) (L)
1497.8783 1497.8277 34 172 185 1 (K) (H)
1497.8783 1497.8575 14 132 145 0 (K) (H)
1513.8945 1513.8525 28 1Met-ox 132 145 0 (K)AGVMALANLLQIQR(H)
2153.2255 2153.1355 42 186 205 0 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (79/244AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/42 matches (11%).
Acc. #: Species: HUMAN Name: Hypothetical protein CGI-99
Index: MW: 28068 Da pI: 6.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1022.6018 1022.5999 1.9 206 213 0 (R) (E)
1483.8683 1483.8120 38 172 185 1 (K) (H)
1483.8683 1483.8273 28 77 89 0 (R) (L)
1497.8783 1497.8575 14 132 145 0 (K) (H)
1513.8945 1513.8525 28 1Met-ox 132 145 0 (K)AGVMALANLLQIQR(H)
2153.2255 2153.1355 42 186 205 0 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 28% (69/244AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/42 matches (14%).
Acc. #: Species: HUMAN Name: HYPOTHETICAL PROTEIN DJ845O24.1
Index: MW: 53386 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1244.6755 1244.6315 35 236 245 0 (R) (E)
1994.0757 1994.1398 -32 AcetN 1 19 1 (-)SLQAPSRLLELAGQSLLR(N)
2031.0794 2031.1175 -19 54 71 0 (K) (T)
2047.0578 2047.1124 -27 1Met-ox 54 71 0 (K)LMVQAWPFLSLPLGSLMK(T)
2063.0531 2063.1074 -26 2Met-ox 54 71 0 (K)LMVQAWPFLSLPLGSLMK(T)
2211.2434 2211.0790 74 309 326 1 (R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (65/459AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/42 matches (14%).
Acc. #: Species: MOUSE Name: Antioxidant protein 2 (1-Cys peroxiredoxin) (1-Cys PRX) (Acidic calcium-independent phospholipase A2) (aiPLA2) (Non-selenium glutathione peroxidase) (NSGPx)
Index: MW: 24871 Da pI: 5.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
906.4552 906.4685 -15 156 162 0 (R) (V)
1191.6885 1191.6737 12 145 155 0 (K) (N)
1409.7133 1409.6735 28 42 53 0 (R) (A)
1755.9150 1755.9679 -30 1Met-ox 107 122 1 (K)GRDLAILLGMLDPVEK(D)
1897.0974 1897.0071 48 68 84 0 (K) (D)
2031.0794 2030.9877 45 25 41 0 (R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (80/224AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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