MS-Fit Search Results

Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D146
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3601 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/125(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	9.346e+011	23 (18)	65.0	18.4	29.5	70.6	38715/6.6	HUMAN	Annexin I (Lipocortin I) (Calpactin II) (Chromobindin 9) (P35) (Phospholipase A2 inhibitory protein)
2	2.299e+008	16 (12)	34.0	12.8	34.8	62.1	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	2.099e+007	16 (12)	27.0	12.8	33.7	41.6	59519/5.1	HUMAN	Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
4	8.100e+006	15 (12)	29.0	12.0	18.5	64.4	65866/8.1	HUMAN	Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
5	1.169e+006	11 (8)	31.0	8.8	20.5	75.3	50255/8.6	MOUSE	ADENYLOSUCCINATE SYNTHETASE, MUSCLE ISOZYME (IMP--ASPARTATE LIGASE) (ADSS) (AMPSASE)

52500	x	.	x	x	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	x	.	x	.	.	.	.	x	x	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.
45313	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.
108237	.	.	x	.	.	.	.	.	x	.	.	.	x	.	.	.	x	.	x	.	.	.	.	x	.	.	.	.	.	.	x	.	.	x	.	.	.	x	.	.	.	.	.	x	.	x	.	x	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.
110808	.	.	.	.	.	.	x	.	.	.	x	x	.	.	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.
103142	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	x	.	x	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 23/125 matches (18%).
Acc. #: Species: HUMAN Name: Annexin I (Lipocortin I) (Calpactin II) (Chromobindin 9) (P35) (Phospholipase A2 inhibitory protein)
Index: MW: 38715 Da pI: 6.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
908.4626	908.4478	16		205	212	0	(R) (R)
966.4654	966.4970	-33	AcetN	1	9	0	(-)AMVSEFLK(Q)
982.5025	982.4919	11	AcetN 1Met-ox	1	9	0	(-)AMVSEFLK(Q)
1064.6051	1064.5489	53		205	213	1	(R) (K)
1087.5623	1087.5168	42	2Met-ox	1	9	0	(-)MAMVSEFLK(Q)
1213.5803	1213.5337	38		167	177	0	(K) (N)
1213.5803	1213.6475	-55	1Met-ox	282	292	1	(K)LHQAMKGVGTR(H)
1262.6505	1262.6017	39		114	124	0	(K) (A)
1370.7178	1370.6778	29		270	281	0	(K) (L)
1387.8211	1387.7684	38		59	71	0	(K) (R)
1543.9449	1543.8695	49		59	72	1	(K) (N)
1550.8930	1550.8178	48		215	228	0	(K) (S)
1606.0416	1605.9580	52		99	113	0	(K) (T)
1678.9939	1678.9128	48		214	228	1	(R) (S)
1702.9742	1702.8863	52		129	144	0	(K) (T)
1734.0175	1734.0529	-20		98	113	1	(K) (T)
1739.8252	1739.7360	51		189	204	0	(R) (A)
1905.1427	1905.0333	57		82	98	1	(K) (A)
1929.9806	1930.0935	-58		54	71	1	(K) (R)
2067.9931	2067.8855	52		186	204	1	(K) (A)
2124.0782	2123.9926	40	pyroGlu	10	26	0	(K)QAWFIENEEQEYVQTVK(S)
2141.1251	2141.0191	50		10	26	0	(K) (S)
2356.3135	2356.1573	66		30	53	0	(K) (A)
2364.2099	2364.1103	42	1Met-ox	318	337	1	(K)MYGISLCQAILDETKGDYEK(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 65% (225/346AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/125 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1033.5462 1033.5166 29 484 492 0 (R) (M)
1087.5623 1087.5285 31 4 12 1 (R) (S)
1159.5889 1159.6547 -57 75 86 1 (K) (G)
1179.6470 1179.6010 39 377 386 0 (K) (H)
1265.6724 1265.6378 27 278 288 0 (R) (K)
1277.7140 1277.6337 63 247 257 1 (K) (N)
1277.7140 1277.7105 2.7 473 483 0 (K) (T)
1302.7143 1302.7018 9.7 393 403 1 (R) (V)
1302.7143 1302.7157 -1.0 344 355 0 (R) (A)
1340.7125 1340.6698 32 365 376 1 (K) (Y)
1383.7418 1383.6909 37 186 197 0 (K) (V)
1475.8270 1475.7494 53 212 223 0 (K) (T)
1475.8270 1475.7858 28 200 211 0 (R) (W)
1638.9467 1638.8604 53 186 199 1 (K) (F)
1716.9795 1716.8517 74 418 432 0 (K) (G)
1986.0237 1985.8949 65 252 267 1 (R) (N)
1994.0758 1993.9772 49 224 239 0 (R) (R)
2150.2013 2150.0783 57 224 240 1 (R) (R)
2384.1178 2383.9524 69 519 549 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
1033.5462	1033.5166	29	484	492	0	(R) (M)
1087.5623	1087.5285	31	4	12	1	(R) (S)
1159.5889	1159.6547	-57	75	86	1	(K) (G)
1179.6470	1179.6010	39	377	386	0	(K) (H)
1265.6724	1265.6378	27	278	288	0	(R) (K)
1277.7140	1277.6337	63	247	257	1	(K) (N)
1277.7140	1277.7105	2.7	473	483	0	(K) (T)
1302.7143	1302.7018	9.7	393	403	1	(R) (V)
1302.7143	1302.7157	-1.0	344	355	0	(R) (A)
1340.7125	1340.6698	32	365	376	1	(K) (Y)
1383.7418	1383.6909	37	186	197	0	(K) (V)
1475.8270	1475.7494	53	212	223	0	(K) (T)
1475.8270	1475.7858	28	200	211	0	(R) (W)
1638.9467	1638.8604	53	186	199	1	(K) (F)
1716.9795	1716.8517	74	418	432	0	(K) (G)
1986.0237	1985.8949	65	252	267	1	(R) (N)
1994.0758	1993.9772	49	224	239	0	(R) (R)
2150.2013	2150.0783	57	224	240	1	(R) (R)
2384.1178	2383.9524	69	519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (219/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 16/125 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
966.4654 966.4573 8.4 195 201 1 (R) (T)
1064.6051 1064.6104 -5.0 157 165 1 (R) (A)
1090.5643 1090.5315 30 148 156 0 (K) (L)
1106.5571 1106.5264 28 1Met-ox 148 156 0 (K)VTMQNLNDR(L)
1109.5356 1109.4904 41 335 343 0 (K) (S)
1201.6294 1201.6177 9.7 246 256 0 (R) (R)
1234.7072 1234.6796 22 236 245 1 (R) (Q)
1262.6505 1262.5977 42 451 464 0 (R) (G)
1300.6336 1300.5956 29 286 295 1 (K) (N)
1340.7125 1340.6923 15 pyroGlu 246 257 1 (R)QSVEADINGLRR(V)
1365.6837 1365.6399 32 323 333 0 (R) (K)
1381.7249 1381.6487 55 166 177 0 (R) (I)
1434.8445 1434.7705 52 440 450 1 (K) (S)
1493.8121 1493.7348 52 323 334 1 (R) (D)
1707.8697 1707.7727 57 41 59 0 (K) (G)
2872.6008 2872.3933 72 296 322 0 (R) (S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
966.4654	966.4573	8.4		195	201	1	(R) (T)
1064.6051	1064.6104	-5.0		157	165	1	(R) (A)
1090.5643	1090.5315	30		148	156	0	(K) (L)
1106.5571	1106.5264	28	1Met-ox	148	156	0	(K)VTMQNLNDR(L)
1109.5356	1109.4904	41		335	343	0	(K) (S)
1201.6294	1201.6177	9.7		246	256	0	(R) (R)
1234.7072	1234.6796	22		236	245	1	(R) (Q)
1262.6505	1262.5977	42		451	464	0	(R) (G)
1300.6336	1300.5956	29		286	295	1	(K) (N)
1340.7125	1340.6923	15	pyroGlu	246	257	1	(R)QSVEADINGLRR(V)
1365.6837	1365.6399	32		323	333	0	(R) (K)
1381.7249	1381.6487	55		166	177	0	(R) (I)
1434.8445	1434.7705	52		440	450	1	(K) (S)
1493.8121	1493.7348	52		323	334	1	(R) (D)
1707.8697	1707.7727	57		41	59	0	(K) (G)
2872.6008	2872.3933	72		296	322	0	(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (161/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 15/125 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 2 epidermal (Cytokeratin 2e) (K2e) (CK 2e)
Index: MW: 65866 Da pI: 8.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1037.5275 1037.5267 0.74 245 253 0 (R) (T)
1082.5737 1082.5999 -24 195 203 1 (K) (F)
1087.5623 1087.6111 -45 186 194 1 (R) (F)
1107.5821 1107.5435 35 360 368 0 (K) (S)
1193.6630 1193.6166 39 381 390 0 (K) (H)
1193.6630 1193.6279 29 244 253 1 (K) (T)
1208.6686 1208.6163 43 282 292 0 (R) (K)
1284.6140 1284.5863 22 1 10 1 (-) (G)
1300.6336 1300.5812 40 1Met-ox 1 10 1 (-)MSCQISCKSR(G)
1320.6370 1320.5834 41 46 61 0 (R) (S)
1329.7333 1329.6399 70 425 436 0 (K) (G)
1329.7333 1329.7266 5.0 348 359 0 (R) (A)
1460.8212 1460.8001 14 309 320 0 (K) (V)
1475.8270 1475.7858 28 204 215 0 (R) (W)
1752.8918 1752.8842 4.3 294 308 1 (K) (V)
1839.0143 1838.9149 54 71 92 0 (K) (G)
2384.1178 2384.2172 -42 468 487 1 (R) (K)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1037.5275	1037.5267	0.74		245	253	0	(R) (T)
1082.5737	1082.5999	-24		195	203	1	(K) (F)
1087.5623	1087.6111	-45		186	194	1	(R) (F)
1107.5821	1107.5435	35		360	368	0	(K) (S)
1193.6630	1193.6166	39		381	390	0	(K) (H)
1193.6630	1193.6279	29		244	253	1	(K) (T)
1208.6686	1208.6163	43		282	292	0	(R) (K)
1284.6140	1284.5863	22		1	10	1	(-) (G)
1300.6336	1300.5812	40	1Met-ox	1	10	1	(-)MSCQISCKSR(G)
1320.6370	1320.5834	41		46	61	0	(R) (S)
1329.7333	1329.6399	70		425	436	0	(K) (G)
1329.7333	1329.7266	5.0		348	359	0	(R) (A)
1460.8212	1460.8001	14		309	320	0	(K) (V)
1475.8270	1475.7858	28		204	215	0	(R) (W)
1752.8918	1752.8842	4.3		294	308	1	(K) (V)
1839.0143	1838.9149	54		71	92	0	(K) (G)
2384.1178	2384.2172	-42		468	487	1	(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (189/645AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/125 matches (8%).
Acc. #: Species: MOUSE Name: ADENYLOSUCCINATE SYNTHETASE, MUSCLE ISOZYME (IMP--ASPARTATE LIGASE) (ADSS) (AMPSASE)
Index: MW: 50255 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1037.5275 1037.5631 -34 165 174 1 (K) (A)
1078.5620 1078.6261 -59 375 384 1 (K) (R)
1458.7790 1458.7593 14 33 46 0 (R) (G)
1636.9597 1636.8521 66 349 363 0 (R) (L)
1716.9795 1716.8709 63 418 431 1 (R) (F)
1716.9795 1716.9437 21 385 398 1 (K) (V)
1723.8803 1723.8154 38 234 248 0 (R) (K)
1739.8252 1739.8103 8.6 1Met-ox 234 248 0 (R)DGVYFMYEALHGPPK(K)
1851.9242 1851.9104 7.4 234 249 1 (R) (V)
1905.1427 1905.0949 25 364 380 1 (K) (L)
2122.1574 2122.0457 53 399 417 1 (K) (K)
2284.2972 2284.1937 45 305 325 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1037.5275	1037.5631	-34		165	174	1	(K) (A)
1078.5620	1078.6261	-59		375	384	1	(K) (R)
1458.7790	1458.7593	14		33	46	0	(R) (G)
1636.9597	1636.8521	66		349	363	0	(R) (L)
1716.9795	1716.8709	63		418	431	1	(R) (F)
1716.9795	1716.9437	21		385	398	1	(K) (V)
1723.8803	1723.8154	38		234	248	0	(R) (K)
1739.8252	1739.8103	8.6	1Met-ox	234	248	0	(R)DGVYFMYEALHGPPK(K)
1851.9242	1851.9104	7.4		234	249	1	(R) (V)
1905.1427	1905.0949	25		364	380	1	(K) (L)
2122.1574	2122.0457	53		399	417	1	(K) (K)
2284.2972	2284.1937	45		305	325	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (144/457AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):