MS-Fit Search Results


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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D152
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1290 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/78(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.259e+00410 (12)20.012.8-6.8074.9 69873/7.8HUMANTyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
23.927e+00412 (15)24.015.4-9.5580.5 53675/5.5HUMANKeratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
31.198e+0049 (11)13.011.5-6.7775.2 73095/7.0HUMANDEAD-box protein 3, Y-chromosomal
41.165e+0048 (10)18.010.3-7.4385.3 60955/8.6HUMANGrowth factor receptor-bound protein 14 (GRB14 adapter protein)
571547 (8)10.09.0-1.8768.3 82806/9.1HUMANPoly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
6424910 (12)17.012.8-3.4667.1 74140/6.6HUMANLamin A/C (70 kDa lamin)
736607 (8)14.09.0-46.142.3 60620/9.8MOUSEAlpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (GalNAc alpha-2,6-sialyltransferase I) (ST6GalNAc I) (Sialyltransferase 7A)
829969 (11)12.011.56.47121 89228/7.2HUMANSemaphorin 3E precursor
929098 (10)12.010.3-8.6656.7 73141/6.7MOUSEPutative ATP-dependent RNA helicase PL10
1025767 (8)15.09.0-33.650.2 42934/8.8HUMANPyruvate dehydrogenase E1 component alpha subunit, testis-specific form, mitochondrial precursor (PDHE1-A type II)
40982x...........x...................x....x...........x.x..............x...x....x.x
28336.....x................x.....x....x.x......x......x...x.....x.x........x..x....
27789......x......x..x...x....................xx..........x.............x....x.....
82218..............x..........x..x............x........x........x........x......x..
112017.x..x.................x....................x....x.....x...x...................
78186..............x..x.....x..............x....x.........x.x...x.x...x............
98072.x.............x....x..............................................x.xx..x....
92776...................x.....x............x.....x..x..x.........x..xx.............
101231.......x.......x.........................xx..........x..x..........x....x.....
66548...x.......x....x....x...............................x..........x........x....


Detailed Results

1. 10/78 matches (12%).
Acc. #: Species: HUMAN Name: Tyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
Index: MW: 69873 Da pI: 7.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
981.4427981.4715-292212280(K) (F)
1014.42911014.3872412Met-ox 3783850(K)ADTEEMMR(E)
1076.50041076.5087-7.65956030(R) (V)
1124.52631124.5523-231201281(R) (Q)
1277.68161277.6101561611700(R) (E)
1307.65911307.7397-622412511(K) (E)
1690.79751690.8189-135805920(K) (M)
1847.90861847.9988-4926411(K) (S)
2211.16242211.1153214314500(R) (Y)
2233.13232233.1399-3.43343531(K) (Q)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 20% (124/619AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/78 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 8 (Cytokeratin 8) (K8) (CK 8)
Index: MW: 53675 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
995.4302995.4468-171Met-ox 3053120(K)TEISEMNR(N)
1045.54951045.5530-3.31501580(R) (L)
1066.45381066.5169-591791861(K) (T)
1081.52711081.5642-349181(K) (A)
1100.40621100.4570-461Met-ox 1992070(K)DVDEAYMNK(V)
1173.57941173.6367-494734830(K) (-)
1277.68161277.7105-233823920(K) (K)
1320.64901320.6721-182532640(R) (A)
1475.75891475.6987414024140(R) (T)
1566.76211566.7950-211Met-ox 3703811(R)QLREYQELMNVK(L)
1847.90861847.8056561341480(R) (R)
2004.02362003.9068581341491(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 24% (119/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/78 matches (11%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3, Y-chromosomal
Index: MW: 73095 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
996.4929996.4387541011090(R) (E)
1018.43381018.4958-6147551(K) (D)
1027.42321027.41557.656640(K) (D)
1040.48841040.5165-2779870(R) (G)
1157.63151157.5876383623710(R) (G)
1173.57941173.5825-2.71Met-ox 3623710(R)IVEQDTMPPK(G)
1320.64901320.6800-232752850(R) (K)
1707.81501707.8964-483103241(R) (L)
1940.98111940.97920.993343491(K) (M)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 13% (88/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/78 matches (10%).
Acc. #: Species: HUMAN Name: Growth factor receptor-bound protein 14 (GRB14 adapter protein)
Index: MW: 60955 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1020.47941020.5301-503313380(R) (L)
1052.59301052.6104-1749580(R) (G)
1066.45381066.5223-644364430(R) (I)
1157.63151157.6166131211310(R) (D)
1282.60871282.5408531Met-ox 3213300(K)MLCAEEEQSR(T)
1475.75891475.8044-314744861(K) (H)
1712.79701712.8165-113703850(R) (S)
2211.16242211.0577474144350(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (98/540AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/78 matches (8%).
Acc. #: Species: HUMAN Name: Poly(A) polymerase gamma (PAP gamma) (Polynucleotide adenylyltransferase gamma) (SRP RNA 3' adenylating enzyme)
Index: MW: 82806 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
982.4949982.49193.03343410(R) (Q)
990.4352990.5049-70961030(K) (L)
1045.54951045.54642.94694770(R) (E)
1179.58571179.55342773820(K) (N)
1271.61931271.6418-181101210(K) (H)
1371.78811371.7484293423540(K) (S)
1450.66311450.6450125365490(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (74/736AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 10/78 matches (12%).
Acc. #: Species: HUMAN Name: Lamin A/C (70 kDa lamin)
Index: MW: 74140 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1020.47941020.4897-101Met-ox 181(-)METPSQRR(A)
1032.46711032.5148-461821890(K) (R)
1048.46691048.5097-411Met-ox 1821890(K)QLQDEMLR(R)
1131.48351131.5282-3933410(K) (L)
1179.58571179.5138616556640(R) (-)
1320.64901320.64096.14284391(R) (V)
1320.64901320.6582-7.01Met-ox 1912011(R)VDAENRLQTMK(E)
1377.62351377.6334-7.25165270(K) (T)
1475.75891475.7681-6.21Met-ox 3673781(K)LALDMEIHAYRK(L)
1566.76211566.75127.06286440(R) (S)
1687.94831687.8727459251(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 17% (116/664AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/78 matches (8%).
Acc. #: Species: MOUSE Name: Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (GalNAc alpha-2,6-sialyltransferase I) (ST6GalNAc I) (Sialyltransferase 7A)
Index: MW: 60620 Da pI: 9.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
982.4949982.5474-533573650(R) (D)
1024.42121024.4700-483273350(K) (T)
1040.48841040.5390-494014080(R) (N)
1707.81501707.9394-731031171(R) (A)
1763.82791763.9404-641581741(K) (R)
1847.90861847.9503-2341560(R) (A)
2004.02362004.0514-1440561(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (75/526AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 9/78 matches (11%).
Acc. #: Species: HUMAN Name: Semaphorin 3E precursor
Index: MW: 89228 Da pI: 7.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1037.46231037.5056-425475550(R) (R)
1052.59301052.5489423863951(K) (D)
1131.48351131.5581-662692780(R) (I)
1181.58001181.6319-44921010(K) (M)
1255.70681255.6283632142231(R) (E)
1282.60871282.7020-737617701(R) (H)
1544.83891544.7466603383500(R) (A)
1614.90941614.8280507127240(K) (V)
1657.77781657.6652681Met-ox 6786901(K)VEDMFNKDDEEDR(H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (98/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 8/78 matches (10%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
999.4167999.4383-221011090(R) (G)
1024.42121024.4547-3325350(R) (G)
1157.63151157.5876383633720(R) (G)
1173.57941173.5825-2.71Met-ox 3633720(R)IVEQDTMPPK(G)
1320.64901320.6800-232762860(R) (K)
1424.69121424.6626201Met-ox 2172291(K)RDLMACAQTGSGK(T)
1707.81501707.8964-483113251(R) (L)
1940.98111940.97920.993353501(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (85/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 7/78 matches (8%).
Acc. #: Species: HUMAN Name: Pyruvate dehydrogenase E1 component alpha subunit, testis-specific form, mitochondrial precursor (PDHE1-A type II)
Index: MW: 42934 Da pI: 8.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
985.3995985.4631-652342420(R) (R)
1011.41541011.4392-241481560(K) (N)
1027.42321027.4341-111Met-ox 1481560(K)GGSMHMYTK(N)
1043.40841043.4290-202Met-ox 1481560(K)GGSMHMYTK(N)
1320.64901320.6986-382762860(K) (Y)
1657.77781657.8947-711Met-ox 3133261(R)DPIIILQDRMVNSK(L)
2004.02362004.0411-8.72442611(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (61/388AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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