MS-Fit Search Results

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D183
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 1894 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/88(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	1.726e+006	14 (15)	32.0	15.9	-73.9	36.1	54168/6.1	HUMAN	Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP) (P-52)
2	2.867e+005	10 (11)	23.0	11.4	-30.2	92.5	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	9.959e+004	13 (14)	26.0	14.8	-37.9	79.2	69873/7.8	HUMAN	Tyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
4	3.868e+004	13 (14)	21.0	14.8	-19.5	89.6	74140/6.6	HUMAN	Lamin A/C (70 kDa lamin)
5	2.684e+004	8 (9)	12.0	9.1	-23.3	99.6	69501/8.2	HUMAN	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>

109265	.	x	.	.	.	.	.	x	x	x	x	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.
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40982	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	x	.	.	.	.	.
78186	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	x	.	.	.	x	x	.	x	.	.	x	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.
83345	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 14/88 matches (15%).
Acc. #: Species: HUMAN Name: Mitochondrial processing peptidase beta subunit, mitochondrial precursor (Beta-MPP) (P-52)
Index: MW: 54168 Da pI: 6.1

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1014.3749	1014.4501	-74	1Met-ox	425	431	0	(R)QMLCYNR(R)
1048.4616	1048.5461	-81		60	68	0	(R) (V)
1085.5303	1085.6206	-83		210	219	0	(R) (S)
1095.3992	1095.4716	-66		482	489	1	(R) (-)
1101.4884	1101.5904	-93		442	451	0	(R) (E)
1111.3922	1111.4665	-67	1Met-ox	482	489	1	(R)SNMCWLRD(-)
1193.6062	1193.7006	-79		432	441	1	(R) (I)
1365.5999	1365.6684	-50		388	399	0	(R) (A)
1434.6321	1434.7269	-66		137	148	1	(R) (D)
1434.6321	1434.7745	-99		16	28	0	(R) (G)
1677.7591	1677.8600	-60		225	238	1	(K) (I)
1713.8134	1713.9135	-58		44	59	0	(R) (V)
1751.8322	1752.0060	-99		462	478	1	(R) (Q)
1891.9755	1892.0493	-39		239	257	0	(R) (F)
1982.9130	1983.0987	-94		42	59	1	(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (159/489AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/88 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1143.4596 1143.5356 -66 1Met-ox 289 298 1 (K)KDVDGAYMTK(V)
1179.5599 1179.6010 -35 377 386 0 (K) (H)
1300.5331 1300.5302 2.2 258 267 0 (K) (N)
1332.6243 1332.5200 78 2Met-ox 258 267 0 (K)NMQDMVEDYR(N)
1475.6886 1475.7494 -41 212 223 0 (K) (T)
1475.6886 1475.7858 -66 200 211 0 (R) (W)
1638.7227 1638.8604 -84 186 199 1 (K) (F)
1687.8499 1687.9707 -72 66 82 1 (R) (G)
1751.8322 1751.8272 2.8 603 624 1 (R) (S)
1993.9417 1993.9772 -18 224 239 0 (R) (R)
2383.8719 2383.9524 -34 519 549 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1143.4596	1143.5356	-66	1Met-ox	289	298	1	(K)KDVDGAYMTK(V)
1179.5599	1179.6010	-35		377	386	0	(K) (H)
1300.5331	1300.5302	2.2		258	267	0	(K) (N)
1332.6243	1332.5200	78	2Met-ox	258	267	0	(K)NMQDMVEDYR(N)
1475.6886	1475.7494	-41		212	223	0	(K) (T)
1475.6886	1475.7858	-66		200	211	0	(R) (W)
1638.7227	1638.8604	-84		186	199	1	(K) (F)
1687.8499	1687.9707	-72		66	82	1	(R) (G)
1751.8322	1751.8272	2.8		603	624	1	(R) (S)
1993.9417	1993.9772	-18		224	239	0	(R) (R)
2383.8719	2383.9524	-34		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (154/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/88 matches (14%).
Acc. #: Species: HUMAN Name: Tyrosine-protein kinase ZAP-70 (70 kDa zeta-associated protein) (Syk-related tyrosine kinase)
Index: MW: 69873 Da pI: 7.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1014.3749 1014.3872 -12 2Met-ox 378 385 0 (K)ADTEEMMR(E)
1140.4827 1140.5472 -56 1Met-ox 120 128 1 (R)DAMVRDYVR(Q)
1302.5533 1302.5966 -33 485 496 0 (K) (S)
1365.5999 1365.7167 -85 473 484 1 (R) (A)
1379.5185 1379.6452 -92 1Met-ox 593 603 1 (R)MRACYYSLASK(V)
1497.6278 1497.7313 -69 505 515 1 (K) (F)
1648.7752 1648.7819 -4.0 284 298 0 (R) (I)
1804.8347 1804.8830 -27 283 298 1 (R) (I)
1826.7914 1826.9223 -72 386 400 0 (R) (L)
1891.9755 1891.8761 53 161 175 1 (R) (K)
1993.9417 1993.9554 -6.9 1Met-ox 580 594 1 (K)WEDRPDFLTVEQRMR(A)
2211.0261 2211.1153 -40 431 450 0 (R) (Y)
2233.0357 2233.1399 -47 334 353 1 (K) (Q)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1014.3749	1014.3872	-12	2Met-ox	378	385	0	(K)ADTEEMMR(E)
1140.4827	1140.5472	-56	1Met-ox	120	128	1	(R)DAMVRDYVR(Q)
1302.5533	1302.5966	-33		485	496	0	(K) (S)
1365.5999	1365.7167	-85		473	484	1	(R) (A)
1379.5185	1379.6452	-92	1Met-ox	593	603	1	(R)MRACYYSLASK(V)
1497.6278	1497.7313	-69		505	515	1	(K) (F)
1648.7752	1648.7819	-4.0		284	298	0	(R) (I)
1804.8347	1804.8830	-27		283	298	1	(R) (I)
1826.7914	1826.9223	-72		386	400	0	(R) (L)
1891.9755	1891.8761	53		161	175	1	(R) (K)
1993.9417	1993.9554	-6.9	1Met-ox	580	594	1	(K)WEDRPDFLTVEQRMR(A)
2211.0261	2211.1153	-40		431	450	0	(R) (Y)
2233.0357	2233.1399	-47		334	353	1	(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (162/619AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/88 matches (14%).
Acc. #: Species: HUMAN Name: Lamin A/C (70 kDa lamin)
Index: MW: 74140 Da pI: 6.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1025.5135 1025.4508 61 2Met-ox 344 351 1 (R)EMAEMRAR(M)
1048.4616 1048.5097 -46 1Met-ox 182 189 0 (K)QLQDEMLR(R)
1179.5599 1179.5138 39 655 664 0 (R) (-)
1374.5736 1374.6290 -40 250 260 0 (R) (K)
1434.6321 1434.6721 -28 585 597 0 (R) (A)
1475.6886 1475.7681 -54 1Met-ox 367 378 1 (K)LALDMEIHAYRK(L)
1536.7662 1536.6753 59 458 470 0 (K) (R)
1566.6511 1566.7512 -64 628 644 0 (R) (S)
1604.7411 1604.7768 -22 436 450 1 (R) (F)
1677.7591 1677.8052 -27 583 597 1 (R) (A)
1687.8499 1687.8727 -14 9 25 1 (R) (I)
1708.6919 1708.7713 -46 1Met-ox 458 471 1 (K)SNEDQSMGNWQIKR(Q)
1961.8521 1961.9932 -72 33 48 1 (K) (V)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1025.5135	1025.4508	61	2Met-ox	344	351	1	(R)EMAEMRAR(M)
1048.4616	1048.5097	-46	1Met-ox	182	189	0	(K)QLQDEMLR(R)
1179.5599	1179.5138	39		655	664	0	(R) (-)
1374.5736	1374.6290	-40		250	260	0	(R) (K)
1434.6321	1434.6721	-28		585	597	0	(R) (A)
1475.6886	1475.7681	-54	1Met-ox	367	378	1	(K)LALDMEIHAYRK(L)
1536.7662	1536.6753	59		458	470	0	(K) (R)
1566.6511	1566.7512	-64		628	644	0	(R) (S)
1604.7411	1604.7768	-22		436	450	1	(R) (F)
1677.7591	1677.8052	-27		583	597	1	(R) (A)
1687.8499	1687.8727	-14		9	25	1	(R) (I)
1708.6919	1708.7713	-46	1Met-ox	458	471	1	(K)SNEDQSMGNWQIKR(Q)
1961.8521	1961.9932	-72		33	48	1	(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (143/664AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/88 matches (9%).
Acc. #: Species: HUMAN Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2 (PAPS synthethase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK2) (SK 2) [Includes: Sulfate adenylyltransferase (Sulfate adenylate transferase) (SAT) (ATP-sulfurylase); Adenylylsulfate kinase (>
Index: MW: 69501 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1085.5303 1085.4726 53 561 569 0 (K) (H)
1101.4884 1101.4675 19 1Met-ox 561 569 0 (K)AMDFYDPAR(H)
1179.5599 1179.5468 11 513 523 0 (R) (K)
1307.5715 1307.6418 -54 513 524 1 (R) (D)
1314.6400 1314.6846 -34 600 609 1 (K) (S)
1497.6278 1497.7562 -86 15 27 0 (K) (N)
1940.8617 1940.8878 -13 174 190 0 (K) (V)
2082.9511 2083.1228 -82 252 269 0 (K) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1085.5303	1085.4726	53		561	569	0	(K) (H)
1101.4884	1101.4675	19	1Met-ox	561	569	0	(K)AMDFYDPAR(H)
1179.5599	1179.5468	11		513	523	0	(R) (K)
1307.5715	1307.6418	-54		513	524	1	(R) (D)
1314.6400	1314.6846	-34		600	609	1	(K) (S)
1497.6278	1497.7562	-86		15	27	0	(K) (N)
1940.8617	1940.8878	-13		174	190	0	(K) (V)
2082.9511	2083.1228	-82		252	269	0	(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (79/614AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):