MS-Fit Search Results


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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): D187
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( HUMAN MOUSE ) selects 13762 entries.
Combined molecular weight, pI and species searches select 12196 entries.
Pre searches select 12196 entries.

Data Set 1 Results

MS-Fit search selects 3136 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/113(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.246e+01321 (18)33.018.645.2100 83140/6.6HUMANGlycyl-tRNA synthetase (Glycine--tRNA ligase) (GlyRS)
21.963e+00616 (14)21.014.249.868.5 76870/6.6HUMANProtein kinase C, beta type (PKC-beta) (PKC-B)
31.478e+00611 (9)16.09.741.768.9 98923/7.3MOUSEOxysterol binding protein-related protein 5 (OSBP-related protein 5) (ORP-5) (Oxystyrol-binding protein homologue 1)
41.443e+00614 (12)30.012.454.258.1 60045/8.1HUMANKeratin, type II cytoskeletal 6A (Cytokeratin 6A) (CK 6A) (K6A keratin)
51.439e+00614 (12)30.012.454.258.1 60201/8.1HUMANKeratin, type II cytoskeletal 6C (Cytokeratin 6C) (CK 6C) (K6C keratin)
61.188e+00616 (14)25.014.235.973.7 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
74.298e+0057 (6)26.06.244.951.9 52563/5.9HUMANBleomycin hydrolase (BLM hydrolase) (BMH) (BH)
84.118e+00516 (14)27.014.231.567.7 73141/6.7MOUSEPutative ATP-dependent RNA helicase PL10
93.215e+00514 (12)24.012.431.568.1 73102/6.7MOUSEDEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
103.166e+00513 (11)29.011.552.755.1 59999/8.1HUMANKeratin, type II cytoskeletal 6B (Cytokeratin 6B) (CK 6B) (K6B keratin)
64615.....x....xx..x.......xx.x......xx.....x.x.............x....x.x.......x..............xx..x......x.x...........x..
86357.x.........x.x...............x.....x......x...x....x...x.x.x............x..xx.x........x.........................
84120...........x...xx.....x.............x.....x...x..................x.......................x..x...x................
84318............x.x.x...x.......xx..x.x...xx........x..........x..............xx.....................................
50658............x.x.x...x.......xx..x.x...xx........x..........x..............xx.....................................
3370.x......xxx...x....x....x..xx..........x.....x.................x........x.........x....x..........x..............
65965..............................................x.........................x...................x......x.........xx.x
98092.x......x.x...x....x....x..xx...x......x.....x.................x....x...x.........x....x.........................
65272.x......x.x...x....x....x..xx..........x.....x.................x........x.........x....x.........................
62377............x.x.x...x.......xx..x.x...x.........x..........x..............xx.....................................


Detailed Results

1. 21/113 matches (18%).
Acc. #: Species: HUMAN Name: Glycyl-tRNA synthetase (Glycine--tRNA ligase) (GlyRS)
Index: MW: 83140 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1044.63151044.5591691591660(K) (Q)
1093.60631093.6985-844784870(K) (E)
1104.65241104.6166323103181(K) (L)
1165.67931165.6880-7.51100(-) (G)
1247.73451247.6636574915010(K) (G)
1296.64171296.5465734654740(R) (A)
1307.75001307.8639-8724341(R) (S)
1391.83521391.7436663193310(K) (N)
1396.84291396.7113947237341(R) (E)
1447.90771447.8888134784901(K) (T)
1457.85691457.7833506036150(R) (C)
1640.89831640.7743764514640(K) (S)
1679.95881679.8368735845960(R) (E)
1695.94201695.8317651Met-ox 5845960(R)IMYTVFEHTFHVR(E)
1782.02151781.9226553653790(K) (A)
2064.24212064.0476942652830(K) (T)
2080.14282080.0425481Met-ox 2652830(K)SPITGNDLSPPVSFNLMFK(T)
2088.18262088.0990403193371(K) (S)
2218.31512218.1760635645830(K) (I)
2235.33382235.1774703924120(R) (I)
2825.69542825.4449892843090(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (244/739AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 16/113 matches (14%).
Acc. #: Species: HUMAN Name: Protein kinase C, beta type (PKC-beta) (PKC-B)
Index: MW: 76870 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4435909.4470-3.92692760(K) (L)
1104.65241104.59145530381(K) (F)
1144.56801144.5533135835921(K) (D)
1350.78951350.7125571Met-ox 5675781(K)EAVAICKGLMTK(H)
1413.79911413.7452381Met-ox 3393500(K)LTDFNFLMVLGK(G)
1470.80051470.7263504694810(K) (I)
1486.82351486.7212691Met-ox 4694810(K)LDNVMLDSEGHIK(I)
1567.86451567.7465753213341(K) (D)
1640.89831640.7862685966071(K) (L)
1656.87461656.8857-6.73373501(R) (G)
1672.90281672.8806131Met-ox 3373501(R)MKLTDFNFLMVLGK(G)
1839.02511838.85049563760(K) (R)
1874.02441873.9886193393551(K) (V)
1890.17181889.98351001Met-ox 3393551(K)LTDFNFLMVLGKGSFGK(V)
1898.02691897.8602881190(-) (F)
2083.14682083.0071672172321(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (146/671AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/113 matches (9%).
Acc. #: Species: MOUSE Name: Oxysterol binding protein-related protein 5 (OSBP-related protein 5) (ORP-5) (Oxystyrol-binding protein homologue 1)
Index: MW: 98923 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1104.65241104.5723735885970(K) (L)
1171.58971171.58940.33pyroGlu 1Met-ox 3763850(R)QLRPGMDLSR(V)
1172.64481172.6210203763850(R) (V)
1247.73451247.6418741Met-ox 8308400(R)HLSTMLSSTVR(A)
1419.75071419.7708-148418540(R) (S)
1470.80051470.7336455405530(R) (V)
1486.82351486.7286641Met-ox 5405530(R)GILYGTMTMELGGK(V)
1486.82351486.7807295986091(R) (E)
1713.93831713.7754957357500(R) (H)
2088.18262088.02497659771(R) (L)
2123.14122123.0919238128290(K) (H)
2218.31512218.2826151411581(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (142/874AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 14/113 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6A (Cytokeratin 6A) (CK 6A) (K6A keratin)
Index: MW: 60045 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1107.62051107.5435703393470(K) (S)
1165.67931165.5853813603690(K) (H)
1172.64481172.63885.12232321(R) (G)
1203.70461203.6374561952040(K) (T)
1329.78641329.7266453273380(R) (A)
1350.78951350.6766843393491(K) (A)
1350.78951350.7269462612721(R) (D)
1391.83521391.7898334564671(K) (L)
1407.81181407.7120712882990(K) (A)
1438.78631438.64979543590(R) (S)
1447.90771447.7756915345500(R) (Y)
1516.78431516.7906-4.21Met-ox 3793901(K)QEIAEINRMIQR(L)
1672.90281672.8077574014150(K) (G)
1858.98461858.9048433603751(K) (N)
1874.02441873.944842pyroGlu 2082220(R)QNLEPLFEQYINNLR(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (171/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 14/113 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6C (Cytokeratin 6C) (CK 6C) (K6C keratin)
Index: MW: 60201 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1107.62051107.5435703393470(K) (S)
1165.67931165.5853813603690(K) (H)
1172.64481172.63885.12232321(R) (G)
1203.70461203.6374561952040(K) (T)
1329.78641329.7266453273380(R) (A)
1350.78951350.6766843393491(K) (A)
1350.78951350.7269462612721(R) (D)
1391.83521391.7898334564671(K) (L)
1407.81181407.7120712882990(K) (A)
1438.78631438.64979543590(R) (S)
1447.90771447.7756915345500(R) (Y)
1516.78431516.7906-4.21Met-ox 3793901(K)QEIAEINRMIQR(L)
1672.90281672.8077574014150(K) (G)
1858.98461858.9048433603751(K) (N)
1874.02441873.944842pyroGlu 2082220(R)QNLEPLFEQYINNLR(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (171/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 16/113 matches (14%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4435909.4174291Met-ox 3273330(R)LVDMMER(G)
1089.56641089.6057-362002080(R) (H)
1090.60131090.5421544194270(K) (R)
1093.60631093.5530493433510(K) (M)
1165.67931165.65941738461(R) (E)
1194.66671194.6417213163260(R) (L)
1300.69241300.6398404794881(R) (S)
1313.71691313.6022871Met-ox 3773870(R)HTMMFSATFPK(E)
1313.71691313.6887213633731(R) (G)
1329.78641329.6837771Met-ox 3633731(R)RIVEQDTMPPK(G)
1447.90771447.8273564284401(K) (D)
1485.81071485.7735251Met-ox 3643761(R)IVEQDTMPPKGVR(H)
1485.81071485.8793-462642761(R) (E)
1707.92041707.8964143123261(R) (L)
1839.02511838.9513405325481(R) (N)
1941.06021940.9792423363511(K) (M)
2083.14682083.0249595125280(K) (I)
2235.33382235.1145984084271(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (171/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/113 matches (6%).
Acc. #: Species: HUMAN Name: Bleomycin hydrolase (BLM hydrolase) (BMH) (BH)
Index: MW: 52563 Da pI: 5.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1486.82351486.7364592562670(R) (I)
1839.02511838.9805242412551(K) (E)
2123.14122123.0384481081241(K) (K)
2257.22652257.18421949680(R) (S)
2812.54602812.49711744681(R) (S)
2825.69542825.45088718420(K) (R)
2844.61432844.4428601321560(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (119/455AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 16/113 matches (14%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4435909.4174291Met-ox 3263320(R)LVDMMER(G)
1089.56641089.6057-361992070(R) (H)
1093.60631093.5530493423500(K) (M)
1165.67931165.65941738461(R) (E)
1194.66671194.6417213153250(R) (L)
1300.69241300.6398404784871(R) (S)
1313.71691313.6022871Met-ox 3763860(R)HTMMFSATFPK(E)
1313.71691313.6887213623721(R) (G)
1329.78641329.6837771Met-ox 3623721(R)RIVEQDTMPPK(G)
1391.83521391.8150154404511(K) (G)
1447.90771447.8273564274391(K) (D)
1485.81071485.7735251Met-ox 3633751(R)IVEQDTMPPKGVR(H)
1485.81071485.8793-462632751(R) (E)
1707.92041707.8964143113251(R) (L)
1765.87751765.7741595996151(R) (A)
1839.02511838.9513405315471(R) (N)
1941.06021940.9792423353501(K) (M)
2083.14682083.0249595115270(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (180/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 14/113 matches (12%).
Acc. #: Species: MOUSE Name: DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
Index: MW: 73102 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4435909.4066411021100(R) (G)
909.4435909.4174291Met-ox 3273330(R)LVDMMER(G)
1089.56641089.6057-362002080(R) (H)
1093.60631093.5530493433510(K) (M)
1165.67931165.65941738461(R) (E)
1194.66671194.6417213163260(R) (L)
1300.69241300.6398404794881(R) (S)
1313.71691313.6022871Met-ox 3773870(R)HTMMFSATFPK(E)
1313.71691313.6887213633731(R) (G)
1329.78641329.6837771Met-ox 3633731(R)RIVEQDTMPPK(G)
1447.90771447.8273564284401(K) (D)
1485.81071485.7735251Met-ox 3643761(R)IVEQDTMPPKGVR(H)
1485.81071485.8793-462642761(R) (E)
1707.92041707.8964143123261(R) (L)
1839.02511838.9513405325481(R) (N)
1941.06021940.9792423363511(K) (M)
2083.14682083.0249595125280(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (160/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 13/113 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 6B (Cytokeratin 6B) (CK 6B) (K6B keratin)
Index: MW: 59999 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1107.62051107.5435703393470(K) (S)
1165.67931165.5853813603690(K) (H)
1172.64481172.63885.12232321(R) (G)
1203.70461203.6374561952040(K) (T)
1329.78641329.7266453273380(R) (A)
1350.78951350.6766843393491(K) (A)
1350.78951350.7269462612721(R) (D)
1391.83521391.73956917301(R) (S)
1391.83521391.7898334564671(K) (L)
1407.81181407.7120712882990(K) (A)
1438.78631438.64979543590(R) (S)
1516.78431516.7906-4.21Met-ox 3793901(K)QEIAEINRMIQR(L)
1672.90281672.8077574014150(K) (G)
1858.98461858.9048433603751(K) (N)
1874.02441873.944842pyroGlu 2082220(R)QNLEPLFEQYINNLR(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (168/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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