MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.8.17.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): F01B
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 113193
Molecular weight search (1000 - 100000 Da) selects 106912 entries.
Full pI range: 113193 entries.
Species search ( MAMMALS ) selects 20817 entries.
Combined molecular weight, pI and species searches select 18748 entries.
Pre searches select 18748 entries.

Data Set 1 Results

MS-Fit search selects 49 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/33(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	9.923e+006	13 (39)	23.0	39.4	8.04	77.6		69294/5.8		BOVIN	Serum albumin precursor (Allergen Bos d 6)
2	506	6 (18)	11.0	18.2	29.6	60.0		69189/5.8		SHEEP	Serum albumin precursor
3	314	4 (12)	8.0	12.1	50.3	102		77359/8.3		CAPHI	Lactotransferrin precursor (Lactoferrin)
4	158	4 (12)	7.0	12.1	10.8	76.3		69367/5.9		HUMAN	Serum albumin precursor
5	113	4 (12)	9.0	12.1	44.2	56.6		47411/6.8		BOVIN	Lactadherin precursor (Milk fat globule-EGF factor 8) (MFG-E8) (MGP57/53) (PAS-6/PAS-7 glycoprotein) (MFGM) (Sperm surface protein SP47) (BP47) (Components 15/16)

79329	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	x	x	x	x	x	.	x	.	x	x	x	x
30161	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	x	.	.	.	.	.	x	.	x
37219	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	x	x	.
14899	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x
50006	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.

Detailed Results

1. 13/33 matches (39%).
Acc. #: Species: BOVIN Name: Serum albumin precursor (Allergen Bos d 6)
Index: MW: 69294 Da pI: 5.8

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
927.4523	927.4940	-45		161	167	0	(K) (R)
1163.6156	1163.6312	-13		66	75	0	(K) (T)
1166.5654	1166.5298	31		499	507	0	(K) (R)
1194.4208	1194.5247	-87		460	468	0	(R) (M)
1305.7229	1305.7167	4.8		402	412	0	(K) (Q)
1415.6805	1415.6881	-5.4	1Met-ox	569	580	0	(K)TVMENFVAFVDK(C)
1439.8240	1439.8123	8.2		360	371	1	(R) (L)
1479.8262	1479.7960	20		421	433	0	(K) (Y)
1567.7828	1567.7433	25		347	359	0	(K) (R)
1640.0027	1639.9383	39		437	451	1	(R) (S)
1888.9979	1888.9274	37		169	183	0	(R) (Y)
1895.0095	1894.9373	38		508	523	0	(R) (A)
2045.1338	2045.0285	51		168	183	1	(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (143/607AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/33 matches (18%).
Acc. #: Species: SHEEP Name: Serum albumin precursor
Index: MW: 69189 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1166.5654 1166.5298 31 499 507 0 (K) (R)
1305.7229 1305.6183 80 300 309 0 (K) (S)
1305.7229 1305.7167 4.8 402 412 0 (K) (K)
1415.6805 1415.6881 -5.4 1Met-ox 569 580 0 (K)TVMENFVAFVDK(C)
1439.8240 1439.8123 8.2 360 371 1 (R) (L)
1888.9979 1888.9274 37 169 183 0 (R) (Y)
2045.1338 2045.0285 51 168 183 1 (R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (70/607AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/33 matches (12%).
Acc. #: Species: CAPHI Name: Lactotransferrin precursor (Lactoferrin)
Index: MW: 77359 Da pI: 8.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1439.8240 1439.6919 92 302 314 0 (K) (R)
1501.7994 1501.6819 78 171 183 0 (R) (A)
1888.9979 1889.0397 -22 623 639 0 (R) (N)
1895.0095 1894.9081 53 241 255 1 (K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (58/708AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/33 matches (12%).
Acc. #: Species: HUMAN Name: Serum albumin precursor
Index: MW: 69367 Da pI: 5.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
927.4523 927.4940 -45 162 168 0 (K) (R)
1166.5654 1166.5298 31 500 508 0 (K) (R)
1640.0027 1639.9383 39 438 452 1 (K) (N)
2045.1338 2045.0959 19 397 413 0 (K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (48/609AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/33 matches (12%).
Acc. #: Species: BOVIN Name: Lactadherin precursor (Milk fat globule-EGF factor 8) (MFG-E8) (MGP57/53) (PAS-6/PAS-7 glycoprotein) (MFGM) (Sperm surface protein SP47) (BP47) (Components 15/16)
Index: MW: 47411 Da pI: 6.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
801.4309 801.4107 25 278 284 0 (K) (Q)
842.4686 842.4525 19 140 146 0 (R) (L)
1439.8240 1439.7072 81 349 360 0 (R) (V)
1501.7994 1501.7222 51 383 395 1 (K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (39/427AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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