MS-Fit Search Results

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Sample ID (comment): T007
Database searched: SwissProt.7.2.2001
Molecular weight search (1000 - 100000 Da) selects 93834 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 10399 entries.
Pre searches select 10399 entries.
MS-Fit search selects 2858 entries (results displayed for top 5 matches).

Parameters Used in Search

4	75.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	4.07e+006	17/60 (28%)	72116.2 / 5.03	HUMAN	78 KD GLUCOSE-REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN HEAVY CHAIN BINDING PROTEIN) (BIP)
2	5.48e+005	16/60 (26%)	72422.6 / 5.07	MOUSE	78 KDA GLUCOSE-REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN HEAVY CHAIN BINDING PROTEIN) (BIP)
3	1.78e+005	15/60 (25%)	60045.4 / 8.09	HUMAN	KERATIN, TYPE II CYTOSKELETAL 6A (CYTOKERATIN 6A) (CK 6A) (K6A KERATIN)
4	1.78e+005	15/60 (25%)	60200.6 / 8.09	HUMAN	KERATIN, TYPE II CYTOSKELETAL 6C (CYTOKERATIN 6C) (CK 6C) (K6C KERATIN)
5	1.08e+005	14/60 (23%)	59999.4 / 8.09	HUMAN	KERATIN, TYPE II CYTOSKELETAL 6B (CYTOKERATIN 6B) (CK 6B) (K6B KERATIN)

Detailed Results

1. 17/60 matches (28%). 72116.2 Da, pI = 5.03. Acc. # P11021. HUMAN. 78 KD GLUCOSE-REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN HEAVY CHAIN BINDING PROTEIN) (BIP).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
986.5435	986.5159	28.0580	532	539	(R)(M)
1107.5864	1107.6050	-16.8252	547	555	(K)(E)
1228.6617	1228.6286	26.9327	50	60	(R)(I)
1430.7385	1430.6916	32.7940	102	113	(R)(F)
1460.8249	1460.7597	44.6833	353	366	(K)(I)
1512.8621	1512.7521	72.7465	324	335	(R)(S)
1528.8143	1528.7470	44.0143	324	335	(R)AKFEELNMDLFR(S)	1Met-ox
1566.8545	1566.7804	47.2907	61	74	(R)(L)
1588.9277	1588.8546	45.9706	352	366	(K)(I)
1816.0609	1815.9969	35.2721	198	214	(R)(E)
1888.0603	1887.9717	46.9367	165	181	(K)(Q)
1920.1511	1920.0343	60.8114	370	385	(K)(G)
1934.1141	1934.0136	51.9543	474	491	(K)(G)
1975.0092	1974.9085	51.0087	601	616	(K)(A)
1999.1889	1999.0864	51.2673	492	509	(R)(V)
2016.1391	2016.0667	35.8987	164	181	(K)(Q)
2165.1195	2164.9926	58.6145	306	323	(R)(A)

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The matched peptides cover 30% (202/653AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 28.3 Mean Error = 42.2017 ppm Data Tolerance = 38.6300 ppm Mean Missed Cleavages = 0.5

2. 16/60 matches (26%). 72422.6 Da, pI = 5.07. Acc. # P20029. MOUSE. 78 KDA GLUCOSE-REGULATED PROTEIN PRECURSOR (GRP 78) (IMMUNOGLOBULIN HEAVY CHAIN BINDING PROTEIN) (BIP).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
986.5435 986.5159 28.0580 534 541 (R)(M)
1107.5864 1107.6050 -16.8252 549 557 (K)(E)
1228.6617 1228.6286 26.9327 51 61 (R)(I)
1430.7385 1430.6916 32.7940 103 114 (R)(F)
1460.8249 1460.7597 44.6833 355 368 (K)(I)
1512.8621 1512.7521 72.7465 326 337 (R)(S)
1528.8143 1528.7470 44.0143 326 337 (R)AKFEELNMDLFR(S) 1Met-ox
1566.8545 1566.7804 47.2907 62 75 (R)(L)
1588.9277 1588.8546 45.9706 354 368 (K)(I)
1816.0609 1815.9969 35.2721 199 215 (R)(E)
1888.0603 1887.9717 46.9367 166 182 (K)(Q)
1920.1511 1920.1179 17.3034 1 18 (-)MMKFTVVAAALLLLGAVR(A) 1Met-ox
1920.1511 1920.0343 60.8114 372 387 (K)(G)
1934.1141 1934.0136 51.9543 476 493 (K)(G)
1975.0092 1974.9085 51.0087 603 618 (K)(A)
1999.1889 1999.0864 51.2673 494 511 (R)(V)
2016.1391 2016.0667 35.8987 165 182 (K)(Q)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
986.5435	986.5159	28.0580	534	541	(R)(M)
1107.5864	1107.6050	-16.8252	549	557	(K)(E)
1228.6617	1228.6286	26.9327	51	61	(R)(I)
1430.7385	1430.6916	32.7940	103	114	(R)(F)
1460.8249	1460.7597	44.6833	355	368	(K)(I)
1512.8621	1512.7521	72.7465	326	337	(R)(S)
1528.8143	1528.7470	44.0143	326	337	(R)AKFEELNMDLFR(S)	1Met-ox
1566.8545	1566.7804	47.2907	62	75	(R)(L)
1588.9277	1588.8546	45.9706	354	368	(K)(I)
1816.0609	1815.9969	35.2721	199	215	(R)(E)
1888.0603	1887.9717	46.9367	166	182	(K)(Q)
1920.1511	1920.1179	17.3034	1	18	(-)MMKFTVVAAALLLLGAVR(A)	1Met-ox
1920.1511	1920.0343	60.8114	372	387	(K)(G)
1934.1141	1934.0136	51.9543	476	493	(K)(G)
1975.0092	1974.9085	51.0087	603	618	(K)(A)
1999.1889	1999.0864	51.2673	494	511	(R)(V)
2016.1391	2016.0667	35.8987	165	182	(K)(Q)

The matched peptides cover 30% (202/655AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 26.7 Mean Error = 39.7716 ppm Data Tolerance = 39.4312 ppm Mean Missed Cleavages = 0.6

3. 15/60 matches (25%). 60045.4 Da, pI = 8.09. Acc. # P02538. HUMAN. KERATIN, TYPE II CYTOSKELETAL 6A (CYTOKERATIN 6A) (CK 6A) (K6A KERATIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
628.3748 628.3782 -5.4308 25 30 (R)(S)
878.4966 878.4583 43.5519 1 9 (-)ASTSTTIR(S) Acet N
1107.5864 1107.5435 38.7361 339 347 (K)(S)
1165.6372 1165.5853 44.5280 360 369 (K)(H)
1172.5675 1172.6388 -60.7488 223 232 (R)(G)
1203.6786 1203.6374 34.2525 195 204 (K)(T)
1263.7500 1263.6949 43.6195 456 466 (K)(K)
1329.7540 1329.7266 20.6198 327 338 (R)(A)
1407.7663 1407.7120 38.5967 288 299 (K)(A)
1455.7601 1455.6868 50.3125 348 359 (R)(Y)
1873.9908 1873.9448 24.5347 208 222 (R)QNLEPLFEQYINNLR(R) pyroGlu
1891.0620 1890.9714 47.9201 208 222 (R)(R)
2047.1620 2047.0725 43.7192 208 223 (R)(Q)
2151.2211 2151.1926 13.2221 165 182 (R)(F)
2350.2189 2350.1825 15.4621 233 252 (R)(Y)

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Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
628.3748	628.3782	-5.4308	25	30	(R)(S)
878.4966	878.4583	43.5519	1	9	(-)ASTSTTIR(S)	Acet N
1107.5864	1107.5435	38.7361	339	347	(K)(S)
1165.6372	1165.5853	44.5280	360	369	(K)(H)
1172.5675	1172.6388	-60.7488	223	232	(R)(G)
1203.6786	1203.6374	34.2525	195	204	(K)(T)
1263.7500	1263.6949	43.6195	456	466	(K)(K)
1329.7540	1329.7266	20.6198	327	338	(R)(A)
1407.7663	1407.7120	38.5967	288	299	(K)(A)
1455.7601	1455.6868	50.3125	348	359	(R)(Y)
1873.9908	1873.9448	24.5347	208	222	(R)QNLEPLFEQYINNLR(R)	pyroGlu
1891.0620	1890.9714	47.9201	208	222	(R)(R)
2047.1620	2047.0725	43.7192	208	223	(R)(Q)
2151.2211	2151.1926	13.2221	165	182	(R)(F)
2350.2189	2350.1825	15.4621	233	252	(R)(Y)

The matched peptides cover 27% (154/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 26.1930 ppm Data Tolerance = 57.4567 ppm Mean Missed Cleavages = 0.6

4. 15/60 matches (25%). 60200.6 Da, pI = 8.09. Acc. # P48666. HUMAN. KERATIN, TYPE II CYTOSKELETAL 6C (CYTOKERATIN 6C) (CK 6C) (K6C KERATIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
628.3748 628.3782 -5.4308 25 30 (R)(S)
878.4966 878.4583 43.5519 1 9 (-)ASTSTTIR(S) Acet N
1107.5864 1107.5435 38.7361 339 347 (K)(S)
1165.6372 1165.5853 44.5280 360 369 (K)(H)
1172.5675 1172.6388 -60.7488 223 232 (R)(G)
1203.6786 1203.6374 34.2525 195 204 (K)(T)
1263.7500 1263.6949 43.6195 456 466 (K)(K)
1329.7540 1329.7266 20.6198 327 338 (R)(A)
1407.7663 1407.7120 38.5967 288 299 (K)(A)
1455.7601 1455.6868 50.3125 348 359 (R)(Y)
1873.9908 1873.9448 24.5347 208 222 (R)QNLEPLFEQYINNLR(R) pyroGlu
1891.0620 1890.9714 47.9201 208 222 (R)(R)
2047.1620 2047.0725 43.7192 208 223 (R)(Q)
2151.2211 2151.1926 13.2221 165 182 (R)(F)
2350.2189 2350.1825 15.4621 233 252 (R)(Y)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
628.3748	628.3782	-5.4308	25	30	(R)(S)
878.4966	878.4583	43.5519	1	9	(-)ASTSTTIR(S)	Acet N
1107.5864	1107.5435	38.7361	339	347	(K)(S)
1165.6372	1165.5853	44.5280	360	369	(K)(H)
1172.5675	1172.6388	-60.7488	223	232	(R)(G)
1203.6786	1203.6374	34.2525	195	204	(K)(T)
1263.7500	1263.6949	43.6195	456	466	(K)(K)
1329.7540	1329.7266	20.6198	327	338	(R)(A)
1407.7663	1407.7120	38.5967	288	299	(K)(A)
1455.7601	1455.6868	50.3125	348	359	(R)(Y)
1873.9908	1873.9448	24.5347	208	222	(R)QNLEPLFEQYINNLR(R)	pyroGlu
1891.0620	1890.9714	47.9201	208	222	(R)(R)
2047.1620	2047.0725	43.7192	208	223	(R)(Q)
2151.2211	2151.1926	13.2221	165	182	(R)(F)
2350.2189	2350.1825	15.4621	233	252	(R)(Y)

The matched peptides cover 27% (154/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 26.1930 ppm Data Tolerance = 57.4567 ppm Mean Missed Cleavages = 0.6

5. 14/60 matches (23%). 59999.4 Da, pI = 8.09. Acc. # P04259. HUMAN. KERATIN, TYPE II CYTOSKELETAL 6B (CYTOKERATIN 6B) (CK 6B) (K6B KERATIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
628.3748 628.3782 -5.4308 25 30 (R)(S)
878.4966 878.4583 43.5519 1 9 (-)ASTSTTIR(S) Acet N
1107.5864 1107.5435 38.7361 339 347 (K)(S)
1165.6372 1165.5853 44.5280 360 369 (K)(H)
1172.5675 1172.6388 -60.7488 223 232 (R)(G)
1203.6786 1203.6374 34.2525 195 204 (K)(T)
1263.7500 1263.6949 43.6195 456 466 (K)(K)
1329.7540 1329.7266 20.6198 327 338 (R)(A)
1407.7663 1407.7120 38.5967 288 299 (K)(A)
1455.7601 1455.6868 50.3125 348 359 (R)(Y)
1873.9908 1873.9448 24.5347 208 222 (R)QNLEPLFEQYINNLR(R) pyroGlu
1891.0620 1890.9714 47.9201 208 222 (R)(R)
2047.1620 2047.0725 43.7192 208 223 (R)(Q)
2151.2211 2151.1926 13.2221 165 182 (R)(F)

Click link below to search for another component.

Click individual mass to do a non-specific cleavage search.

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
628.3748	628.3782	-5.4308	25	30	(R)(S)
878.4966	878.4583	43.5519	1	9	(-)ASTSTTIR(S)	Acet N
1107.5864	1107.5435	38.7361	339	347	(K)(S)
1165.6372	1165.5853	44.5280	360	369	(K)(H)
1172.5675	1172.6388	-60.7488	223	232	(R)(G)
1203.6786	1203.6374	34.2525	195	204	(K)(T)
1263.7500	1263.6949	43.6195	456	466	(K)(K)
1329.7540	1329.7266	20.6198	327	338	(R)(A)
1407.7663	1407.7120	38.5967	288	299	(K)(A)
1455.7601	1455.6868	50.3125	348	359	(R)(Y)
1873.9908	1873.9448	24.5347	208	222	(R)QNLEPLFEQYINNLR(R)	pyroGlu
1891.0620	1890.9714	47.9201	208	222	(R)(R)
2047.1620	2047.0725	43.7192	208	223	(R)(Q)
2151.2211	2151.1926	13.2221	165	182	(R)(F)

The matched peptides cover 23% (134/564AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.3 Mean Error = 26.9595 ppm Data Tolerance = 59.3065 ppm Mean Missed Cleavages = 0.4