MS-Fit Search Results

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Sample ID (comment): T008
Database searched: SwissProt.7.2.2001
Molecular weight search (10000 - 100000 Da) selects 83989 entries.
Full pI range: 99359 entries.
Species search ( HUMAN MOUSE ) selects 11676 entries.
Combined molecular weight, pI and species searches select 9999 entries.
Pre searches select 9999 entries.
MS-Fit search selects 1670 entries (results displayed for top 5 matches).

Parameters Used in Search

4	75.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.3e+003	9/40 (22%)	66018.2 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN)
2	195	10/40 (25%)	64051.5 / 11.39	HUMAN	ALU SUBFAMILY SC SEQUENCE CONTAMINATION WARNING ENTRY
3	178	6/40 (15%)	87297.1 / 7.19	MOUSE	DELTA 1-PYRROLINE-5-CARBOXYLATE SYNTHETASE (P5CS) [INCLUDES: GLUTAMATE 5-KINASE (GAMMA-GLUTAMYL KINASE) (GK); GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GPR) (GLUTAMATE-5-SEMIALDEHYDE DEHYDROGENASE) (GLUTAMYL-GAMMA-SEMIALDEHYDE DEHYDROGENASE)]
4	154	5/40 (12%)	23559.1 / 7.53	MOUSE	RAS-RELATED PROTEIN RAB-7
5	137	6/40 (15%)	44046.5 / 7.02	HUMAN	PUTATIVE METHIONINE AMINOPEPTIDASE 1 (METAP 1) (PEPTIDASE M 1)

Detailed Results

1. 9/40 matches (22%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KDA CYTOKERATIN) (HAIR ALPHA PROTEIN).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6769	1179.6010	64.3481	377	386	(K)(H)
1277.7735	1277.7105	49.2620	473	483	(K)(T)
1475.8480	1475.7858	42.1128	200	211	(R)(W)
1475.8480	1475.7494	66.7694	212	223	(K)(T)
1487.8576	1487.8043	35.8739	240	251	(R)(L)
1657.8929	1657.7934	60.0237	13	29	(R)(R)
1716.9736	1716.8517	71.0100	418	432	(K)(G)
1798.9941	1799.0139	-11.0394	393	407	(R)(L)
1994.0578	1993.9772	40.4116	224	239	(R)(R)
2150.1332	2150.0783	25.5129	224	240	(R)(R)

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The matched peptides cover 18% (120/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 22.5 Mean Error = 44.4285 ppm Data Tolerance = 48.9817 ppm Mean Missed Cleavages = 0.6

2. 10/40 matches (25%). 64051.5 Da, pI = 11.39. Acc. # P39192. HUMAN. ALU SUBFAMILY SC SEQUENCE CONTAMINATION WARNING ENTRY.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1487.8576 1487.9062 -32.6449 463 475 (R)(V)
1515.8390 1515.7556 55.0444 223 236 (R)(I)
1741.9070 1741.8040 59.1475 258 273 (R)QENRLNPGGGGCSEPR(S) 1Cys-am
1780.0250 1779.9944 17.2054 388 402 (R)(Q)
1945.1020 1944.9739 65.8550 516 536 (R)(H)
2001.0656 2000.9758 44.8842 498 515 (R)(L)
2030.1163 2030.0168 49.0136 219 236 (R)(I)
2178.1169 2178.1460 -13.3604 403 422 (R)(R)
2216.1944 2216.2477 -24.0536 23 41 (R)(Y)
2872.4465 2872.5045 -20.1874 451 475 (R)(V)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1487.8576	1487.9062	-32.6449	463	475	(R)(V)
1515.8390	1515.7556	55.0444	223	236	(R)(I)
1741.9070	1741.8040	59.1475	258	273	(R)QENRLNPGGGGCSEPR(S)	1Cys-am
1780.0250	1779.9944	17.2054	388	402	(R)(Q)
1945.1020	1944.9739	65.8550	516	536	(R)(H)
2001.0656	2000.9758	44.8842	498	515	(R)(L)
2030.1163	2030.0168	49.0136	219	236	(R)(I)
2178.1169	2178.1460	-13.3604	403	422	(R)(R)
2216.1944	2216.2477	-24.0536	23	41	(R)(Y)
2872.4465	2872.5045	-20.1874	451	475	(R)(V)

The matched peptides cover 25% (152/585AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 25.0 Mean Error = 20.0904 ppm Data Tolerance = 78.2367 ppm Mean Missed Cleavages = 0.9

3. 6/40 matches (15%). 87297.1 Da, pI = 7.19. Acc. # Q9Z110. MOUSE. DELTA 1-PYRROLINE-5-CARBOXYLATE SYNTHETASE (P5CS) [INCLUDES: GLUTAMATE 5-KINASE (GAMMA-GLUTAMYL KINASE) (GK); GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GPR) (GLUTAMATE-5-SEMIALDEHYDE DEHYDROGENASE) (GLUTAMYL-GAMMA-SEMIALDEHYDE DEHYDROGENASE)].

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1378.7388 1378.8211 -59.6813 17 28 (R)(S)
1475.8480 1475.7422 71.6559 650 662 (K)(S)
1741.9070 1741.8146 53.0915 766 781 (R)(Y)
1994.0578 1994.0679 -5.0982 111 128 (R)(H)
2031.0676 2030.9792 43.5274 606 622 (K)(D)
2872.4465 2872.4450 0.5277 603 626 (R)DSKCEYPAACNALETLLIHRDLLR(T) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1378.7388	1378.8211	-59.6813	17	28	(R)(S)
1475.8480	1475.7422	71.6559	650	662	(K)(S)
1741.9070	1741.8146	53.0915	766	781	(R)(Y)
1994.0578	1994.0679	-5.0982	111	128	(R)(H)
2031.0676	2030.9792	43.5274	606	622	(K)(D)
2872.4465	2872.4450	0.5277	603	626	(R)DSKCEYPAACNALETLLIHRDLLR(T)	1Cys-am

The matched peptides cover 10% (83/795AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.0 Mean Error = 17.3371 ppm Data Tolerance = 96.4679 ppm Mean Missed Cleavages = 0.7

4. 5/40 matches (12%). 23559.1 Da, pI = 7.53. Acc. # P51150. MOUSE. RAS-RELATED PROTEIN RAB-7.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1036.6071 1036.5679 37.7970 39 48 (K)(E)
1475.8480 1475.7535 64.0433 114 126 (R)(I)
1780.0250 1779.9441 45.4675 133 146 (R)QVATKRAQAWCYRK(N) 1Cys-am
2042.1221 2041.9727 73.1722 80 97 (R)GADCCVLVFDVTAPNTFK(T) 2Cys-am
2216.1944 2216.1352 26.7216 114 132 (R)(Q)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1036.6071	1036.5679	37.7970	39	48	(K)(E)
1475.8480	1475.7535	64.0433	114	126	(R)(I)
1780.0250	1779.9441	45.4675	133	146	(R)QVATKRAQAWCYRK(N)	1Cys-am
2042.1221	2041.9727	73.1722	80	97	(R)GADCCVLVFDVTAPNTFK(T)	2Cys-am
2216.1944	2216.1352	26.7216	114	132	(R)(Q)

The matched peptides cover 29% (61/207AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 12.5 Mean Error = 49.4403 ppm Data Tolerance = 37.9992 ppm Mean Missed Cleavages = 0.8

5. 6/40 matches (15%). 44046.5 Da, pI = 7.02. Acc. # P53582. HUMAN. PUTATIVE METHIONINE AMINOPEPTIDASE 1 (METAP 1) (PEPTIDASE M 1).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1277.7735 1277.7119 48.2153 51 61 (K)(K)
1736.9525 1736.9236 16.6245 307 321 (K)(A)
1790.9384 1790.8212 65.4176 186 199 (R)NCYPSPLNYYNFPK(S) 1Cys-am
1837.9183 1837.8253 50.5990 36 50 (K)LGIQGSYFCSQECFK(G) 1Cys-am
2001.0656 2001.0769 -5.6296 217 233 (R)(N)
2343.0609 2343.0089 22.2000 16 35 (R)(L)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1277.7735	1277.7119	48.2153	51	61	(K)(K)
1736.9525	1736.9236	16.6245	307	321	(K)(A)
1790.9384	1790.8212	65.4176	186	199	(R)NCYPSPLNYYNFPK(S)	1Cys-am
1837.9183	1837.8253	50.5990	36	50	(K)LGIQGSYFCSQECFK(G)	1Cys-am
2001.0656	2001.0769	-5.6296	217	233	(R)(N)
2343.0609	2343.0089	22.2000	16	35	(R)(L)

The matched peptides cover 23% (92/394AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 15.0 Mean Error = 32.9045 ppm Data Tolerance = 52.6810 ppm Mean Missed Cleavages = 0.5