MS-Fit Search Results

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Sample ID (comment): HSG006
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 195 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
200.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
3

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
20

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.13e+003	5/20 (25%)	12345.2 / 8.24	HUMAN	MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) (GLYCOSYLATION-INHIBITING FACTOR) (GIF).
2	206	4/20 (20%)	51276.8 / 8.32	HUMAN	PROPERDIN PRECURSOR.
3	195	6/20 (30%)	88886.6 / 5.15	HUMAN	SPLICEOSOME ASSOCIATED PROTEIN 114 (SAP 114) (SF3A120).
4	86.7	5/20 (25%)	41522.9 / 6.08	MOUSE	GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-14 SUBUNIT.
5	85	7/20 (35%)	82945.1 / 8.49	MOUSE	HEPATOCYTE GROWTH FACTOR PRECURSOR (SCATTER FACTOR) (SF) (HEPATOPOEITIN A).

Detailed Results

1. 5/20 matches (25%). 12345.2 Da, pI = 8.24. Acc. # P14174. HUMAN. MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) (GLYCOSYLATION-INHIBITING FACTOR) (GIF)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
715.3959	715.3851	15.0449	67	73	(K)(S)
1058.6227	1058.6032	18.4329	78	86	(K)LLCGLLAER(L)	1Cys-am
1287.7143	1287.6884	20.1155	1	11	(-)(A)
1303.7011	1303.6833	13.6451	1	11	(-)PMFIVNTNVPR(A)	1Met-ox
2995.4204	2995.3685	17.3415	89	114	(R)(-)

15 unmatched masses: 568.1483 656.0787 825.1331 842.5286 1243.0808 1255.0778 1299.7105 1309.6914 1315.7072 1331.6859 1940.9598 2211.1271 2383.9800 2449.0843 2465.0793

The matched peptides cover 45% (52/114 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/20 matches (20%). 51276.8 Da, pI = 8.32. Acc. # P27918. HUMAN. PROPERDIN PRECURSOR..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1331.6859 1331.6312 41.0043 68 79 (R)SGGLCQPCRSPR(W) 1Cys-am
2383.9800 2384.0804 -42.0812 80 100 (R)(Y)
2449.0843 2449.0528 12.8645 380 401 (K)(A)
2465.0793 2465.0477 12.8327 380 401 (K)GSWSEWSTWGLCMPPCGPNPTR(A) 1Met-ox

16 unmatched masses: 568.1483 656.0787 715.3959 825.1331 842.5286 1058.6227 1243.0808 1255.0778 1287.7143 1299.7105 1303.7011 1309.6914 1315.7072 1940.9598 2211.1271 2995.4204

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1331.6859	1331.6312	41.0043	68	79	(R)SGGLCQPCRSPR(W)	1Cys-am
2383.9800	2384.0804	-42.0812	80	100	(R)(Y)
2449.0843	2449.0528	12.8645	380	401	(K)(A)
2465.0793	2465.0477	12.8327	380	401	(K)GSWSEWSTWGLCMPPCGPNPTR(A)	1Met-ox

The matched peptides cover 11% (55/469 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/20 matches (30%). 88886.6 Da, pI = 5.15. Acc. # Q15459. HUMAN. SPLICEOSOME ASSOCIATED PROTEIN 114 (SAP 114) (SF3A120)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
715.3959 715.3375 81.6143 232 237 (K)(E)
842.5286 842.5100 22.1145 701 708 (R)(V)
1058.6227 1058.5305 87.1814 709 717 (K)(T)
1303.7011 1303.5853 88.7644 500 511 (K)(T)
2465.0793 2465.2042 -50.6756 192 210 (R)(L)
2995.4204 2995.5304 -36.7278 392 419 (K)(I)

14 unmatched masses: 568.1483 656.0787 825.1331 1243.0808 1255.0778 1287.7143 1299.7105 1309.6914 1315.7072 1331.6859 1940.9598 2211.1271 2383.9800 2449.0843

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
715.3959	715.3375	81.6143	232	237	(K)(E)
842.5286	842.5100	22.1145	701	708	(R)(V)
1058.6227	1058.5305	87.1814	709	717	(K)(T)
1303.7011	1303.5853	88.7644	500	511	(K)(T)
2465.0793	2465.2042	-50.6756	192	210	(R)(L)
2995.4204	2995.5304	-36.7278	392	419	(K)(I)

The matched peptides cover 10% (82/793 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/20 matches (25%). 41522.9 Da, pI = 6.08. Acc. # P30677. MOUSE. GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-14 SUBUNIT..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1315.7072 1315.5155 145.7103 1 12 (-)MAGCCCLSAEEK(E) 1Cys-am
1331.6859 1331.5104 131.7763 1 12 (-)MAGCCCLSAEEK(E) 1Met-ox 1Cys-am
1940.9598 1940.9176 21.7434 319 334 (K)VIYSHFTCATDTENIR(F) 1Cys-am
2449.0843 2449.3852 -122.8751 35 56 (R)(I)
2465.0793 2465.3802 -122.0263 35 56 (R)ELKLLLLGTGESGKSTFIKQMR(I) 1Met-ox

15 unmatched masses: 568.1483 656.0787 715.3959 825.1331 842.5286 1058.6227 1243.0808 1255.0778 1287.7143 1299.7105 1303.7011 1309.6914 2211.1271 2383.9800 2995.4204

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1315.7072	1315.5155	145.7103	1	12	(-)MAGCCCLSAEEK(E)	1Cys-am
1331.6859	1331.5104	131.7763	1	12	(-)MAGCCCLSAEEK(E)	1Met-ox 1Cys-am
1940.9598	1940.9176	21.7434	319	334	(K)VIYSHFTCATDTENIR(F)	1Cys-am
2449.0843	2449.3852	-122.8751	35	56	(R)(I)
2465.0793	2465.3802	-122.0263	35	56	(R)ELKLLLLGTGESGKSTFIKQMR(I)	1Met-ox

The matched peptides cover 14% (50/355 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/20 matches (35%). 82945.1 Da, pI = 8.49. Acc. # Q08048. MOUSE. HEPATOCYTE GROWTH FACTOR PRECURSOR (SCATTER FACTOR) (SF) (HEPATOPOEITIN A)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1058.6227 1058.4577 155.9393 222 231 (R)(T)
1299.7105 1299.5938 89.8310 347 356 (K)(N)
1303.7011 1303.5047 150.6290 384 394 (K)CDVSSGQDCYR(G) 1Cys-am
1331.6859 1331.6418 33.0830 338 348 (K)(D)
1940.9598 1941.0380 -40.2815 518 534 (K)HICGGSLIKESWVLTAR(Q) 1Cys-am
2211.1271 2211.2171 -40.6946 128 149 (R)(C)
2995.4204 2995.3719 16.2161 357 383 (R)(C)

13 unmatched masses: 568.1483 656.0787 715.3959 825.1331 842.5286 1243.0808 1255.0778 1287.7143 1309.6914 1315.7072 2383.9800 2449.0843 2465.0793

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1058.6227	1058.4577	155.9393	222	231	(R)(T)
1299.7105	1299.5938	89.8310	347	356	(K)(N)
1303.7011	1303.5047	150.6290	384	394	(K)CDVSSGQDCYR(G)	1Cys-am
1331.6859	1331.6418	33.0830	338	348	(K)(D)
1940.9598	1941.0380	-40.2815	518	534	(K)HICGGSLIKESWVLTAR(Q)	1Cys-am
2211.1271	2211.2171	-40.6946	128	149	(R)(C)
2995.4204	2995.3719	16.2161	357	383	(R)(C)

The matched peptides cover 14% (106/728 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):