MS-Fit Search Results

Press stop on your browser if you wish to abort this MS-Fit search prematurely.

Sample ID (comment): HSG060
Database searched: SwissProt.r36
Molecular weight search (1000 - 100000 Da) selects 69977 entries.
Full pI range: 74019 entries.
Species search ( HUMAN MOUSE ) selects 8141 entries.
Combined molecular weight, pI and species searches select 7373 entries.
MS-Fit search selects 982 entries (results displayed for top 5 matches).

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

Min. # Peptides
to Match
4

Peptide Mass
Tolerance (+/-)
50.000 ppm

Peptide Masses
are
monoisotopic

Digest
Used
Trypsin

Max. # Missed
Cleavages
4

Cysteines
Modified by
unmodified

Peptide
N terminus
Hydrogen (H)

Peptide
C terminus
Free Acid (O H)

Input #
Peptide Masses
63

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	1.09e+003	7/63 (11%)	27745.3 / 4.73	HUMAN	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS).
2	1.09e+003	7/63 (11%)	27771.3 / 4.73	MOUSE	14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT).
3	286	4/63 (6%)	48929.6 / 9.48	MOUSE	ACROSIN PRECURSOR (EC 3.4.21.10).
4	227	6/63 (9%)	65886.8 / 8.16	HUMAN	KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN).
5	208	5/63 (7%)	21050.4 / 6.95	HUMAN	OX40 LIGAND (OX40L) (GLYCOPROTEIN GP34) (TAX-TRANSCRIPTIONALLY ACTIVATED GLYCOPROTEIN 1).

Detailed Results

1. 7/63 matches (11%). 27745.3 Da, pI = 4.73. Acc. # P29312. HUMAN. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (FACTOR ACTIVATING EXOENZYME S) (FAS)..

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
615.3673	615.3578	15.4127	50	55	(K)(R)
762.3725	762.3998	-35.7846	69	75	(K)(Q)
1189.6833	1189.6615	18.3144	213	222	(K)(D)
1548.7623	1548.7142	31.0611	28	41	(K)(N)
2041.0389	2040.9878	25.0021	140	157	(K)(K)
2131.9379	2131.9923	-25.5096	194	212	(K)(D)
2169.1563	2169.0828	33.8700	139	157	(K)(K)
2169.1563	2169.0828	33.8700	140	158	(K)(E)

56 unmatched masses: 603.3594 606.3182 608.2150 610.2171 618.1139 625.3841 632.3369 647.2616 672.1462 687.3091 691.4019 712.2581 723.3362 732.2704 842.5232 907.4351 1156.5969 1157.6518 1179.6007 1234.6126 1237.5700 1302.6937 1307.7123 1412.6856 1475.7612 1489.7787 1566.7483 1598.7217 1638.8565 1643.8148 1698.8268 1707.7951 1812.8336 1914.7902 1929.9120 1940.9215 1994.9703 2013.8809 2040.0156 2056.8315 2162.2595 2170.0862 2195.9325 2211.1077 2215.0146 2234.1168 2283.1497 2284.1936 2286.0948 2383.9762 2408.1080 2705.1422 2706.0837 2718.0934 3216.7821 3265.3140

The matched peptides cover 31% (76/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/63 matches (11%). 27771.3 Da, pI = 4.73. Acc. # P35215. MOUSE. 14-3-3 PROTEIN ZETA/DELTA (PROTEIN KINASE C INHIBITOR PROTEIN-1) (KCIP-1) (MITOCHONDRIAL IMPORT STIMULATION FACTOR S1 SUBUNIT)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
615.3673 615.3578 15.4127 50 55 (K)(R)
762.3725 762.3998 -35.7846 69 75 (K)(Q)
1189.6833 1189.6615 18.3144 213 222 (K)(D)
1548.7623 1548.7142 31.0611 28 41 (K)(N)
2041.0389 2040.9878 25.0021 140 157 (K)(K)
2131.9379 2131.9923 -25.5096 194 212 (K)(D)
2169.1563 2169.0828 33.8700 139 157 (K)(K)
2169.1563 2169.0828 33.8700 140 158 (K)(E)

56 unmatched masses: 603.3594 606.3182 608.2150 610.2171 618.1139 625.3841 632.3369 647.2616 672.1462 687.3091 691.4019 712.2581 723.3362 732.2704 842.5232 907.4351 1156.5969 1157.6518 1179.6007 1234.6126 1237.5700 1302.6937 1307.7123 1412.6856 1475.7612 1489.7787 1566.7483 1598.7217 1638.8565 1643.8148 1698.8268 1707.7951 1812.8336 1914.7902 1929.9120 1940.9215 1994.9703 2013.8809 2040.0156 2056.8315 2162.2595 2170.0862 2195.9325 2211.1077 2215.0146 2234.1168 2283.1497 2284.1936 2286.0948 2383.9762 2408.1080 2705.1422 2706.0837 2718.0934 3216.7821 3265.3140

The matched peptides cover 31% (76/245 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/63 matches (6%). 48929.6 Da, pI = 9.48. Acc. # P23578. MOUSE. ACROSIN PRECURSOR (EC 3.4.21.10)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1548.7623 1548.8273 -42.0153 135 148 (K)(I)
2195.9325 2196.0395 -48.7660 202 219 (R)VDLIDLDLCNSTQWYNGR(V) 1Cys-am
2215.0146 2215.0866 -32.5343 149 168 (K)ITPPVTCGNFIGPCCLPHFK(A) 1Cys-am
2286.0948 2286.1237 -12.6521 149 168 (K)ITPPVTCGNFIGPCCLPHFK(A) 2Cys-am

59 unmatched masses: 603.3594 606.3182 608.2150 610.2171 615.3673 618.1139 625.3841 632.3369 647.2616 672.1462 687.3091 691.4019 712.2581 723.3362 732.2704 762.3725 842.5232 907.4351 1156.5969 1157.6518 1179.6007 1189.6833 1234.6126 1237.5700 1302.6937 1307.7123 1412.6856 1475.7612 1489.7787 1566.7483 1598.7217 1638.8565 1643.8148 1698.8268 1707.7951 1812.8336 1914.7902 1929.9120 1940.9215 1994.9703 2013.8809 2040.0156 2041.0389 2056.8315 2131.9379 2162.2595 2169.1563 2170.0862 2211.1077 2234.1168 2283.1497 2284.1936 2383.9762 2408.1080 2705.1422 2706.0837 2718.0934 3216.7821 3265.3140

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1548.7623	1548.8273	-42.0153	135	148	(K)(I)
2195.9325	2196.0395	-48.7660	202	219	(R)VDLIDLDLCNSTQWYNGR(V)	1Cys-am
2215.0146	2215.0866	-32.5343	149	168	(K)ITPPVTCGNFIGPCCLPHFK(A)	1Cys-am
2286.0948	2286.1237	-12.6521	149	168	(K)ITPPVTCGNFIGPCCLPHFK(A)	2Cys-am

The matched peptides cover 11% (52/436 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/63 matches (9%). 65886.8 Da, pI = 8.16. Acc. # P04264. HUMAN. KERATIN, TYPE II CYTOSKELETAL 1 (CYTOKERATIN 1) (K1) (CK 1) (67 KD CYTOKERATIN) (HAIR ALPHA PROTEIN)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1179.6007 1179.6010 -0.2757 376 385 (K)(H)
1302.6937 1302.7157 -16.8838 343 354 (R)(A)
1302.6937 1302.7018 -6.1994 392 402 (R)(V)
1475.7612 1475.7858 -16.6603 199 210 (R)(W)
1475.7612 1475.7494 7.9949 211 222 (K)(T)
1638.8565 1638.8604 -2.3925 185 198 (K)(F)
2286.0948 2286.1254 -13.3668 366 385 (K)(H)
2383.9762 2383.9524 9.9861 518 548 (R)(G)

57 unmatched masses: 603.3594 606.3182 608.2150 610.2171 615.3673 618.1139 625.3841 632.3369 647.2616 672.1462 687.3091 691.4019 712.2581 723.3362 732.2704 762.3725 842.5232 907.4351 1156.5969 1157.6518 1189.6833 1234.6126 1237.5700 1307.7123 1412.6856 1489.7787 1548.7623 1566.7483 1598.7217 1643.8148 1698.8268 1707.7951 1812.8336 1914.7902 1929.9120 1940.9215 1994.9703 2013.8809 2040.0156 2041.0389 2056.8315 2131.9379 2162.2595 2169.1563 2170.0862 2195.9325 2211.1077 2215.0146 2234.1168 2283.1497 2284.1936 2408.1080 2705.1422 2706.0837 2718.0934 3216.7821 3265.3140

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
1179.6007	1179.6010	-0.2757	376	385	(K)(H)
1302.6937	1302.7157	-16.8838	343	354	(R)(A)
1302.6937	1302.7018	-6.1994	392	402	(R)(V)
1475.7612	1475.7858	-16.6603	199	210	(R)(W)
1475.7612	1475.7494	7.9949	211	222	(K)(T)
1638.8565	1638.8604	-2.3925	185	198	(K)(F)
2286.0948	2286.1254	-13.3668	366	385	(K)(H)
2383.9762	2383.9524	9.9861	518	548	(R)(G)

The matched peptides cover 17% (112/643 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/63 matches (7%). 21050.4 Da, pI = 6.95. Acc. # P23510. HUMAN. OX40 LIGAND (OX40L) (GLYCOPROTEIN GP34) (TAX-TRANSCRIPTIONALLY ACTIVATED GLYCOPROTEIN 1)..

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1412.6856 1412.7459 -42.6871 134 146 (R)(D)
1638.8565 1638.8413 9.2596 74 87 (K)(V)
1812.8336 1812.8921 -32.2551 107 121 (K)(D)
2040.0156 2040.0687 -26.0609 71 87 (K)KEKGFILTSQKEDEIMK(V) 1Met-ox
2211.1077 2211.1484 -18.4059 88 106 (K)VQNNSVIINCDGFYLISLK(G) 1Cys-am

58 unmatched masses: 603.3594 606.3182 608.2150 610.2171 615.3673 618.1139 625.3841 632.3369 647.2616 672.1462 687.3091 691.4019 712.2581 723.3362 732.2704 762.3725 842.5232 907.4351 1156.5969 1157.6518 1179.6007 1189.6833 1234.6126 1237.5700 1302.6937 1307.7123 1475.7612 1489.7787 1548.7623 1566.7483 1598.7217 1643.8148 1698.8268 1707.7951 1914.7902 1929.9120 1940.9215 1994.9703 2013.8809 2041.0389 2056.8315 2131.9379 2162.2595 2169.1563 2170.0862 2195.9325 2215.0146 2234.1168 2283.1497 2284.1936 2286.0948 2383.9762 2408.1080 2705.1422 2706.0837 2718.0934 3216.7821 3265.3140

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1412.6856	1412.7459	-42.6871	134	146	(R)(D)
1638.8565	1638.8413	9.2596	74	87	(K)(V)
1812.8336	1812.8921	-32.2551	107	121	(K)(D)
2040.0156	2040.0687	-26.0609	71	87	(K)KEKGFILTSQKEDEIMK(V)	1Met-ox
2211.1077	2211.1484	-18.4059	88	106	(K)VQNNSVIINCDGFYLISLK(G)	1Cys-am

The matched peptides cover 34% (64/183 AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):