MS-Fit Search Results

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Sample ID (comment): HSG081
Database searched: SwissProt.8.20.2001
Molecular weight search (1000 - 100000 Da) selects 94194 entries.
Full pI range: 99754 entries.
Species search ( HUMAN MOUSE ) selects 11713 entries.
Combined molecular weight, pI and species searches select 10428 entries.
Pre searches select 10428 entries.
MS-Fit search selects 401 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	6.51e+005	14/36 (38%)	32660.3 / 5.54	HUMAN	INORGANIC PYROPHOSPHATASE (PYROPHOSPHATE PHOSPHO-HYDROLASE) (PPASE)
2	337	6/36 (16%)	36879.4 / 5.56	HUMAN	ANNEXIN A8 (ANNEXIN VIII) (VASCULAR ANTICOAGULANT-BETA) (VAC-BETA)
3	130	5/36 (13%)	65132.9 / 5.92	HUMAN	CD166 ANTIGEN PRECURSOR (ACTIVATED LEUKOCYTE-CELL ADHESION MOLECULE) (ALCAM)
4	109	6/36 (16%)	36843.5 / 5.56	MOUSE	ANNEXIN A8 (ANNEXIN VIII)
5	88.1	6/36 (16%)	71175.0 / 7.00	HUMAN	FRAGILE X MENTAL RETARDATION 1 PROTEIN (PROTEIN FMR-1) (FMRP)

Detailed Results

1. 14/36 matches (38%). 32660.3 Da, pI = 5.54. Acc. # Q15181. HUMAN. INORGANIC PYROPHOSPHATASE (PYROPHOSPHATE PHOSPHO-HYDROLASE) (PPASE).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
954.4588	954.4169	43.9498	1	9	(-)SGFSTEER(A)	Acet N
1053.5793	1053.5369	40.2690	10	18	(R)(V)
1114.6239	1114.5937	27.1214	80	88	(R)(G)
1685.8457	1685.7879	34.3052	239	253	(K)GISCMNTTLSESPFK(C)	1Cys-am
1694.9403	1694.8906	29.3335	178	191	(R)(R)
1701.8277	1701.7828	26.3856	239	253	(K)GISCMNTTLSESPFK(C)	1Met-ox 1Cys-am
1789.9192	1789.9046	8.1618	140	155	(K)(V)
1851.0100	1850.9917	9.8855	177	191	(K)(R)
1851.0100	1850.9917	9.8855	178	192	(R)(Y)
1863.9053	1863.9281	-12.2374	26	41	(K)(D)
1940.0370	1940.0527	-8.0860	58	74	(K)(K)
2175.9455	2175.9109	15.8891	110	128	(K)HTGCCGDNDPIDVCEIGSK(V)	3Cys-am
2230.1400	2230.0821	25.9788	193	211	(R)(D)
2230.1400	2230.0848	24.7596	234	253	(K)KTNGKGISCMNTTLSESPFK(C)	1Met-ox 1Cys-am
2444.2663	2444.2058	24.7708	156	177	(K)(L)
2461.1823	2461.1213	24.7837	89	109	(K)(H)

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The matched peptides cover 66% (192/289AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 38.9 Mean Error = 20.3222 ppm Data Tolerance = 31.2400 ppm Mean Missed Cleavages = 0.5

2. 6/36 matches (16%). 36879.4 Da, pI = 5.56. Acc. # P13928. HUMAN. ANNEXIN A8 (ANNEXIN VIII) (VASCULAR ANTICOAGULANT-BETA) (VAC-BETA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1172.7132 1172.6825 26.1286 158 167 (R)ILVCLLQGSR(D) 1Cys-am
1539.7548 1539.7014 34.6561 250 261 (K)CTQNLHSYFAER(L) 1Cys-am
1698.8836 1698.8559 16.2999 195 208 (R)GTDEMKFITILCTR(S) 1Cys-am
1727.9816 1727.9656 9.2763 36 51 (K)(S)
1940.0370 1940.1293 -47.5732 113 129 (K)(E)
2740.2409 2740.1750 24.0466 134 157 (K)(I)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1172.7132	1172.6825	26.1286	158	167	(R)ILVCLLQGSR(D)	1Cys-am
1539.7548	1539.7014	34.6561	250	261	(K)CTQNLHSYFAER(L)	1Cys-am
1698.8836	1698.8559	16.2999	195	208	(R)GTDEMKFITILCTR(S)	1Cys-am
1727.9816	1727.9656	9.2763	36	51	(K)(S)
1940.0370	1940.1293	-47.5732	113	129	(K)(E)
2740.2409	2740.1750	24.0466	134	157	(K)(I)

The matched peptides cover 28% (93/327AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 10.4724 ppm Data Tolerance = 59.4569 ppm Mean Missed Cleavages = 0.7

3. 5/36 matches (13%). 65132.9 Da, pI = 5.92. Acc. # Q13740. HUMAN. CD166 ANTIGEN PRECURSOR (ACTIVATED LEUKOCYTE-CELL ADHESION MOLECULE) (ALCAM).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
735.4010 735.3711 40.7055 313 318 (K)(K)
749.3607 749.3868 -34.8223 313 318 (K)CSLIDK(K) 1Cys-am
1694.9403 1694.8713 40.7086 90 104 (R)(I)
1940.0370 1940.0070 15.4341 56 71 (K)(S)
2444.2663 2444.2343 13.1010 473 493 (K)IIISPEENVTLTCTAENQLER(T) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
735.4010	735.3711	40.7055	313	318	(K)(K)
749.3607	749.3868	-34.8223	313	318	(K)CSLIDK(K)	1Cys-am
1694.9403	1694.8713	40.7086	90	104	(R)(I)
1940.0370	1940.0070	15.4341	56	71	(K)(S)
2444.2663	2444.2343	13.1010	473	493	(K)IIISPEENVTLTCTAENQLER(T)	1Cys-am

The matched peptides cover 9% (58/583AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 13.9 Mean Error = 15.0254 ppm Data Tolerance = 61.7071 ppm Mean Missed Cleavages = 0.2

4. 6/36 matches (16%). 36843.5 Da, pI = 5.56. Acc. # O35640. MOUSE. ANNEXIN A8 (ANNEXIN VIII).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1114.6239 1114.5832 36.5852 293 301 (K)(T)
1172.7132 1172.6825 26.1286 158 167 (R)ILVCLLQGSR(D) 1Cys-am
1539.7548 1539.7127 27.3600 250 261 (K)(L)
1727.9816 1727.9656 9.2763 36 51 (K)(S)
1940.0370 1940.1293 -47.5732 113 129 (K)(E)
2740.2409 2740.1750 24.0466 134 157 (K)(I)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1114.6239	1114.5832	36.5852	293	301	(K)(T)
1172.7132	1172.6825	26.1286	158	167	(R)ILVCLLQGSR(D)	1Cys-am
1539.7548	1539.7127	27.3600	250	261	(K)(L)
1727.9816	1727.9656	9.2763	36	51	(K)(S)
1940.0370	1940.1293	-47.5732	113	129	(K)(E)
2740.2409	2740.1750	24.0466	134	157	(K)(I)

The matched peptides cover 26% (88/327AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 12.6373 ppm Data Tolerance = 61.5730 ppm Mean Missed Cleavages = 1.0

5. 6/36 matches (16%). 71175.0 Da, pI = 7.00. Acc. # Q06787. HUMAN. FRAGILE X MENTAL RETARDATION 1 PROTEIN (PROTEIN FMR-1) (FMRP).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
735.4010 735.3902 14.7374 472 477 (R)(N)
1539.7548 1539.7013 34.7749 552 564 (K)(N)
1566.7864 1566.8143 -17.7755 394 407 (K)(D)
1707.8486 1707.8311 10.2797 139 152 (R)QMCAKEAAHKDFKK(A) 1Met-ox
2444.2663 2444.2430 9.5289 124 143 (K)(E)
2444.2663 2444.1952 29.0864 573 593 (R)TTDGSLQIRVDCNNERSVHTK(T) 1Cys-am
2740.2409 2740.2816 -14.8752 89 111 (K)GEFYVIEYAACDATYNEIVTIER(L) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
735.4010	735.3902	14.7374	472	477	(R)(N)
1539.7548	1539.7013	34.7749	552	564	(K)(N)
1566.7864	1566.8143	-17.7755	394	407	(K)(D)
1707.8486	1707.8311	10.2797	139	152	(R)QMCAKEAAHKDFKK(A)	1Met-ox
2444.2663	2444.2430	9.5289	124	143	(K)(E)
2444.2663	2444.1952	29.0864	573	593	(R)TTDGSLQIRVDCNNERSVHTK(T)	1Cys-am
2740.2409	2740.2816	-14.8752	89	111	(K)GEFYVIEYAACDATYNEIVTIER(L)	1Cys-am

The matched peptides cover 16% (106/632AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.7 Mean Error = 9.3938 ppm Data Tolerance = 39.9041 ppm Mean Missed Cleavages = 1.3