MS-Fit Search Results

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Sample ID (comment): HSG90
Database searched: SwissProt.1.20.2002
Molecular weight search (1000 - 100000 Da) selects 98142 entries.
Full pI range: 103987 entries.
Species search ( HUMAN MOUSE ) selects 12504 entries.
Combined molecular weight, pI and species searches select 11102 entries.
Pre searches select 11102 entries.
MS-Fit search selects 1258 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	3	carbamidomethylation	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	6.88e+005	16/49 (32%)	51805.0 / 5.35	HUMAN	P59 PROTEIN (HSP BINDING IMMUNOPHILIN) (HBI) (POSSIBLE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE) (PPIASE) (ROTAMASE) (FKBP52 PROTEIN) (52 KDA FK506 BINDING PROTEIN) (P52) (FKBP59) (HSP56)
2	4.67e+003	10/49 (20%)	66018.2 / 8.16	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	623	12/49 (24%)	82437.2 / 9.14	HUMAN	ZINC FINGER PROTEIN 225
4	604	8/49 (16%)	51632.1 / 6.43	MOUSE	P59 PROTEIN (HSP BINDING IMMUNOPHILIN) (HBI) (POSSIBLE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE) (PPIASE) (ROTAMASE) (FKBP52 PROTEIN) (52 KDA FK506 BINDING PROTEIN) (FKBP59)
5	369	7/49 (14%)	29665.8 / 9.26	HUMAN	SURFEIT LOCUS PROTEIN 2 (SURF-2)

Detailed Results

1. 16/49 matches (32%). 51805.0 Da, pI = 5.35. Acc. # Q02790. HUMAN. P59 PROTEIN (HSP BINDING IMMUNOPHILIN) (HBI) (POSSIBLE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE) (PPIASE) (ROTAMASE) (FKBP52 PROTEIN) (52 KDA FK506 BINDING PROTEIN) (P52) (FKBP59) (HSP56).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
678.3286	678.3575	-42.5472	182	186	(K)(E)
921.4431	921.4794	-39.3782	180	186	(K)(E)
1043.4780	1043.4984	-19.6197	410	417	(K)(L)
1117.5640	1117.5788	-13.2677	391	399	(K)TQLAVCQQR(I)	1Cys-am
1156.6140	1156.6366	-19.5456	235	244	(K)(Y)
1171.5770	1171.5934	-13.9623	409	417	(K)(L)
1452.7252	1452.7157	6.5656	332	344	(K)LQAFSAAIESCNK(A)	1Cys-am
1541.7909	1541.7712	12.7417	360	373	(R)(A)
1582.8275	1582.8481	-13.0300	75	88	(K)(A)
1635.8212	1635.8535	-19.7317	53	66	(R)(F)
1697.8756	1697.8723	1.9064	359	373	(R)(A)
1940.1095	1940.0646	23.1463	235	250	(K)(S)
1950.9760	1950.9449	15.9323	190	206	(R)(A)
2197.1233	2197.2021	-35.8687	233	250	(K)(S)
2366.2160	2366.1550	25.7950	255	274	(K)(E)
2535.2329	2535.2012	12.5103	99	121	(K)VGEVCHITCKPEYAYGSAGSPPK(I)	2Cys-am

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The matched peptides cover 34% (159/459AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 32.7 Mean Error = -7.3971 ppm Data Tolerance = 44.0897 ppm Mean Missed Cleavages = 0.6

2. 10/49 matches (20%). 66018.2 Da, pI = 8.16. Acc. # P04264. HUMAN. Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
678.3286 678.3422 -20.0578 617 624 (R)(S)
1179.5784 1179.6010 -19.1115 377 386 (K)(H)
1277.6734 1277.6337 31.0076 247 257 (K)(N)
1277.6734 1277.7105 -29.0969 473 483 (K)(T)
1475.7692 1475.7858 -11.2724 200 211 (R)(W)
1475.7692 1475.7494 13.3830 212 223 (K)(T)
1487.8416 1487.8043 25.1064 240 251 (R)(L)
1638.8701 1638.8604 5.9270 186 199 (K)(F)
1716.8875 1716.8517 20.8671 418 432 (K)(G)
1942.0331 1941.9882 23.1238 444 460 (K)(L)
1993.9996 1993.9772 11.2378 224 239 (R)(R)
2383.9890 2383.9524 15.3510 519 549 (R)(G)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)
678.3286	678.3422	-20.0578	617	624	(R)(S)
1179.5784	1179.6010	-19.1115	377	386	(K)(H)
1277.6734	1277.6337	31.0076	247	257	(K)(N)
1277.6734	1277.7105	-29.0969	473	483	(K)(T)
1475.7692	1475.7858	-11.2724	200	211	(R)(W)
1475.7692	1475.7494	13.3830	212	223	(K)(T)
1487.8416	1487.8043	25.1064	240	251	(R)(L)
1638.8701	1638.8604	5.9270	186	199	(K)(F)
1716.8875	1716.8517	20.8671	418	432	(K)(G)
1942.0331	1941.9882	23.1238	444	460	(K)(L)
1993.9996	1993.9772	11.2378	224	239	(R)(R)
2383.9890	2383.9524	15.3510	519	549	(R)(G)

The matched peptides cover 25% (164/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.4 Mean Error = 5.5388 ppm Data Tolerance = 40.5025 ppm Mean Missed Cleavages = 0.5

3. 12/49 matches (24%). 82437.2 Da, pI = 9.14. Acc. # Q9UK10. HUMAN. ZINC FINGER PROTEIN 225.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1043.4780 1043.5274 -47.4018 441 448 (R)(V)
1171.5770 1171.5352 35.6871 626 634 (K)(W)
1475.7692 1475.7317 25.4169 617 628 (R)(C)
1582.8275 1582.7760 32.5132 329 342 (K)SALNSHRMVHTGEK(R) 1Met-ox
1638.8701 1638.8175 32.1111 184 196 (K)SFCYSSALRIHQR(V) 1Cys-am
2040.0638 2039.9894 36.4678 127 144 (K)QDDMPCQVDAGLSIIHVK(T) 1Cys-am
2146.1056 2146.0286 35.9005 588 604 (K)(F)
2197.1233 2197.0507 33.0311 234 251 (K)(K)
2211.1707 2211.0664 47.1962 234 251 (K)CEQCGKGFSRRSGLYVHR(K) 1Cys-am
2211.1707 2211.0803 40.9009 617 634 (R)(W)
2215.1113 2215.0024 49.1524 362 379 (K)HQIDHTGEKPYNCKECGK(S) 1Cys-am
2283.1847 2283.0837 44.2469 281 298 (R)(S)
2366.2160 2366.2199 -1.6326 299 317 (R)(C)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1043.4780	1043.5274	-47.4018	441	448	(R)(V)
1171.5770	1171.5352	35.6871	626	634	(K)(W)
1475.7692	1475.7317	25.4169	617	628	(R)(C)
1582.8275	1582.7760	32.5132	329	342	(K)SALNSHRMVHTGEK(R)	1Met-ox
1638.8701	1638.8175	32.1111	184	196	(K)SFCYSSALRIHQR(V)	1Cys-am
2040.0638	2039.9894	36.4678	127	144	(K)QDDMPCQVDAGLSIIHVK(T)	1Cys-am
2146.1056	2146.0286	35.9005	588	604	(K)(F)
2197.1233	2197.0507	33.0311	234	251	(K)(K)
2211.1707	2211.0664	47.1962	234	251	(K)CEQCGKGFSRRSGLYVHR(K)	1Cys-am
2211.1707	2211.0803	40.9009	617	634	(R)(W)
2215.1113	2215.0024	49.1524	362	379	(K)HQIDHTGEKPYNCKECGK(S)	1Cys-am
2283.1847	2283.0837	44.2469	281	298	(R)(S)
2366.2160	2366.2199	-1.6326	299	317	(R)(C)

The matched peptides cover 22% (161/706AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 24.5 Mean Error = 27.9684 ppm Data Tolerance = 51.8725 ppm Mean Missed Cleavages = 1.8

4. 8/49 matches (16%). 51632.1 Da, pI = 6.43. Acc. # P30416. MOUSE. P59 PROTEIN (HSP BINDING IMMUNOPHILIN) (HBI) (POSSIBLE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE) (PPIASE) (ROTAMASE) (FKBP52 PROTEIN) (52 KDA FK506 BINDING PROTEIN) (FKBP59).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
678.3286 678.3575 -42.5472 182 186 (R)(E)
1043.4780 1043.4984 -19.6197 410 417 (K)(L)
1117.5640 1117.5788 -13.2677 391 399 (K)TQLAVCQQR(T) 1Cys-am
1171.5770 1171.5934 -13.9623 409 417 (K)(L)
1452.7252 1452.7157 6.5656 332 344 (K)LQAFSAAIESCNK(A) 1Cys-am
1582.8275 1582.8481 -13.0300 75 88 (K)(A)
1635.8212 1635.8535 -19.7317 53 66 (R)(F)
2233.1221 2233.1473 -11.3140 213 232 (R)(R)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
678.3286	678.3575	-42.5472	182	186	(R)(E)
1043.4780	1043.4984	-19.6197	410	417	(K)(L)
1117.5640	1117.5788	-13.2677	391	399	(K)TQLAVCQQR(T)	1Cys-am
1171.5770	1171.5934	-13.9623	409	417	(K)(L)
1452.7252	1452.7157	6.5656	332	344	(K)LQAFSAAIESCNK(A)	1Cys-am
1582.8275	1582.8481	-13.0300	75	88	(K)(A)
1635.8212	1635.8535	-19.7317	53	66	(R)(F)
2233.1221	2233.1473	-11.3140	213	232	(R)(R)

The matched peptides cover 18% (84/458AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 16.3 Mean Error = -15.8634 ppm Data Tolerance = 27.0791 ppm Mean Missed Cleavages = 0.5

5. 7/49 matches (14%). 29665.8 Da, pI = 9.26. Acc. # Q15527. HUMAN. SURFEIT LOCUS PROTEIN 2 (SURF-2).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1043.4780 1043.5097 -30.3845 77 84 (K)(L)
1452.7252 1452.7559 -21.1236 14 25 (R)(K)
1541.7909 1541.7899 0.6602 119 131 (K)QGVEYVPACLVHR(R) 1Cys-am
1697.8756 1697.8910 -9.0643 119 132 (K)QGVEYVPACLVHRR(R) 1Cys-am
1940.1095 1940.0466 32.3945 10 25 (R)(K)
2535.2329 2535.2237 3.6484 113 132 (K)YEECQKQGVEYVPACLVHRR(R) 1Cys-am
2705.2545 2705.3404 -31.7652 113 133 (K)YEECQKQGVEYVPACLVHRRR(R) 2Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1043.4780	1043.5097	-30.3845	77	84	(K)(L)
1452.7252	1452.7559	-21.1236	14	25	(R)(K)
1541.7909	1541.7899	0.6602	119	131	(K)QGVEYVPACLVHR(R)	1Cys-am
1697.8756	1697.8910	-9.0643	119	132	(K)QGVEYVPACLVHRR(R)	1Cys-am
1940.1095	1940.0466	32.3945	10	25	(R)(K)
2535.2329	2535.2237	3.6484	113	132	(K)YEECQKQGVEYVPACLVHRR(R)	1Cys-am
2705.2545	2705.3404	-31.7652	113	133	(K)YEECQKQGVEYVPACLVHRRR(R)	2Cys-am

The matched peptides cover 17% (45/256AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 14.3 Mean Error = -7.9478 ppm Data Tolerance = 45.3254 ppm Mean Missed Cleavages = 1.3