MS-Fit Search Results

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Sample ID (comment): HSG316
Database searched: SwissProt.8.28.2000
Molecular weight search (1000 - 100000 Da) selects 83787 entries.
Full pI range: 88628 entries.
Species search ( HUMAN MOUSE ) selects 9907 entries.
Combined molecular weight, pI and species searches select 8922 entries.
Pre searches select 8922 entries.
MS-Fit search selects 642 entries (results displayed for top 5 matches).

Parameters Used in Search

Considered modifications: | Oxidation of M | Protein N-terminus Acetylated | Acrylamide Modified Cys |

4	50.000 ppm	monoisotopic	Trypsin	4	unmodified	Hydrogen (H)	Free Acid (O H)
Min. # Peptides to Match	Peptide Mass Tolerance (+/-)	Peptide Masses are	Digest Used	Max. # Missed Cleavages	Cysteines Modified by	Peptide N terminus	Peptide C terminus

Result Summary

Rank	MOWSE Score	# (%) Masses Matched	Protein MW (Da)/pI	Species	Protein Name
1	2.13e+006	18/34 (52%)	56665.1 / 6.29	HUMAN	D-3-PHOSPHOGLYCERATE DEHYDROGENASE (PGDH)
2	328	7/34 (20%)	51449.0 / 6.51	MOUSE	D-3-PHOSPHOGLYCERATE DEHYDROGENASE (PGDH) (A10)
3	205	8/34 (23%)	76365.1 / 5.74	HUMAN	CGMP-DEPENDENT PROTEIN KINASE 1, ALPHA ISOZYME (CGK 1 ALPHA) (CGKI-ALPHA)
4	142	9/34 (26%)	65375.2 / 11.31	HUMAN	ALU SUBFAMILY SB2 SEQUENCE CONTAMINATION WARNING ENTRY
5	122	4/34 (11%)	71289.3 / 6.02	MOUSE	GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)

Detailed Results

1. 18/34 matches (52%). 56665.1 Da, pI = 6.29. Acc. # O43175. HUMAN. D-3-PHOSPHOGLYCERATE DEHYDROGENASE (PGDH).

m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
671.4294	671.4204	13.3035	70	75	(K)(A)
733.4017	733.3997	2.7716	1	7	(-)AFANLR(K)	Acet N
899.5816	899.5678	15.3470	147	155	(K)(I)
986.6212	986.6039	17.5344	462	469	(R)(T)
1099.6159	1099.6111	4.2845	237	247	(R)(A)
1390.6780	1390.7000	-15.8395	120	132	(R)QIPQATASMKDGK(W)	1Met-ox
1390.6780	1390.6544	16.9715	523	533	(K)(-)
1462.7263	1462.7034	15.6362	9	20	(K)VLISDSLDPCCR(K)	2Cys-am
1590.8153	1590.7984	10.6677	8	20	(R)KVLISDSLDPCCR(K)	2Cys-am
1590.8153	1590.7984	10.6677	9	21	(K)VLISDSLDPCCRK(I)	2Cys-am
1638.8262	1638.7871	23.8613	295	308	(R)CGEEIAVQFVDMVK(G)	1Cys-am
1639.8763	1639.9205	-26.9878	365	380	(K)NAGNCLSPAVIVGLLK(E)	1Cys-am
1654.7980	1654.7821	9.6363	295	308	(R)CGEEIAVQFVDMVK(G)	1Met-ox 1Cys-am
1900.9993	1900.9690	15.9191	39	54	(K)EELIAELQDCEGLIVR(S)	1Cys-am
2231.1275	2231.0767	22.7974	248	268	(R)ALQSGQCAGAALDVFTEEPPR(D)	1Cys-am
2272.2089	2272.1681	17.9380	470	491	(R)(L)
2288.2322	2288.1808	22.4476	39	58	(K)EELIAELQDCEGLIVRSATK(V)	1Cys-am
2288.2322	2288.1630	30.2088	470	491	(R)TQTSDPAMLPTMIGLLAEAGVR(L)	1Met-ox
2304.1982	2304.1579	17.4910	470	491	(R)TQTSDPAMLPTMIGLLAEAGVR(L)	2Met-ox
2471.3302	2471.2816	19.6790	34	54	(K)QNLSKEELIAELQDCEGLIVR(S)	1Cys-am
2502.2451	2502.2047	16.1524	248	270	(R)ALQSGQCAGAALDVFTEEPPRDR(A)	1Cys-am

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The matched peptides cover 33% (179/533AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 52.9 Mean Error = 12.4042 ppm Data Tolerance = 26.0074 ppm Mean Missed Cleavages = 0.3

2. 7/34 matches (20%). 51449.0 Da, pI = 6.51. Acc. # Q61753. MOUSE. D-3-PHOSPHOGLYCERATE DEHYDROGENASE (PGDH) (A10).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
671.4294 671.4204 13.3035 22 27 (K)(A)
899.5816 899.5678 15.3470 99 107 (K)(I)
1099.6159 1099.6111 4.2845 189 199 (R)(A)
1638.8262 1638.7871 23.8613 247 260 (R)CGEEIAVQFVDMVK(G) 1Cys-am
1654.7980 1654.7821 9.6363 247 260 (R)CGEEIAVQFVDMVK(G) 1Met-ox 1Cys-am
2231.1275 2231.0767 22.7974 200 220 (R)ALQSGQCAGAALDVFTEEPPR(D) 1Cys-am
2502.2451 2502.2047 16.1524 200 222 (R)ALQSGQCAGAALDVFTEEPPRDR(A) 1Cys-am

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
671.4294	671.4204	13.3035	22	27	(K)(A)
899.5816	899.5678	15.3470	99	107	(K)(I)
1099.6159	1099.6111	4.2845	189	199	(R)(A)
1638.8262	1638.7871	23.8613	247	260	(R)CGEEIAVQFVDMVK(G)	1Cys-am
1654.7980	1654.7821	9.6363	247	260	(R)CGEEIAVQFVDMVK(G)	1Met-ox 1Cys-am
2231.1275	2231.0767	22.7974	200	220	(R)ALQSGQCAGAALDVFTEEPPR(D)	1Cys-am
2502.2451	2502.2047	16.1524	200	222	(R)ALQSGQCAGAALDVFTEEPPRDR(A)	1Cys-am

The matched peptides cover 12% (63/485AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 20.6 Mean Error = 15.0546 ppm Data Tolerance = 13.8376 ppm Mean Missed Cleavages = 0.1

3. 8/34 matches (23%). 76365.1 Da, pI = 5.74. Acc. # Q13976. HUMAN. CGMP-DEPENDENT PROTEIN KINASE 1, ALPHA ISOZYME (CGK 1 ALPHA) (CGKI-ALPHA).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1373.6414 1373.6741 -23.8309 345 356 (K)(L)
1390.6780 1390.6490 20.8260 271 282 (R)(T)
1590.8153 1590.8426 -17.1469 43 57 (K)(T)
1639.8763 1639.9093 -20.1380 381 394 (K)(R)
1640.9181 1640.8720 28.0852 597 611 (R)(D)
1661.8925 1661.8797 7.6511 43 57 (K)CQSVLPVPSTHIGPR(T) 1Cys-am
1795.9642 1796.0104 -25.7451 381 395 (K)(H)
2211.1389 2211.1041 15.7249 1 18 (-)(I)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1373.6414	1373.6741	-23.8309	345	356	(K)(L)
1390.6780	1390.6490	20.8260	271	282	(R)(T)
1590.8153	1590.8426	-17.1469	43	57	(K)(T)
1639.8763	1639.9093	-20.1380	381	394	(K)(R)
1640.9181	1640.8720	28.0852	597	611	(R)(D)
1661.8925	1661.8797	7.6511	43	57	(K)CQSVLPVPSTHIGPR(T)	1Cys-am
1795.9642	1796.0104	-25.7451	381	395	(K)(H)
2211.1389	2211.1041	15.7249	1	18	(-)(I)

The matched peptides cover 12% (87/671AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 23.5 Mean Error = -1.8217 ppm Data Tolerance = 44.2851 ppm Mean Missed Cleavages = 1.4

4. 9/34 matches (26%). 65375.2 Da, pI = 11.31. Acc. # P39191. HUMAN. ALU SUBFAMILY SB2 SEQUENCE CONTAMINATION WARNING ENTRY.

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
899.5816 899.5678 15.3470 53 60 (R)(L)
1273.6245 1273.5999 19.2580 466 477 (R)(S)
1462.7263 1462.7688 -29.0588 166 178 (R)MAXTREAELAVSR(D) 1Met-ox
1590.8153 1590.7910 15.3155 171 185 (R)(S)
1639.8763 1639.8080 41.6326 253 268 (R)(E)
1661.8925 1661.8281 38.7266 171 185 (R)EAELAVSRDCATAVR(S) 1Cys-am
2211.1389 2211.1053 15.2028 61 80 (R)LRQENGVNPGSGACSEPRLR(H) 1Cys-am
2288.2322 2288.2435 -4.9505 513 535 (R)(V)
2304.1982 2304.1710 11.8317 433 455 (R)(A)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
899.5816	899.5678	15.3470	53	60	(R)(L)
1273.6245	1273.5999	19.2580	466	477	(R)(S)
1462.7263	1462.7688	-29.0588	166	178	(R)MAXTREAELAVSR(D)	1Met-ox
1590.8153	1590.7910	15.3155	171	185	(R)(S)
1639.8763	1639.8080	41.6326	253	268	(R)(E)
1661.8925	1661.8281	38.7266	171	185	(R)EAELAVSRDCATAVR(S)	1Cys-am
2211.1389	2211.1053	15.2028	61	80	(R)LRQENGVNPGSGACSEPRLR(H)	1Cys-am
2288.2322	2288.2435	-4.9505	513	535	(R)(V)
2304.1982	2304.1710	11.8317	433	455	(R)(A)

The matched peptides cover 20% (122/603AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 26.5 Mean Error = 13.7005 ppm Data Tolerance = 42.5716 ppm Mean Missed Cleavages = 1.0

5. 4/34 matches (11%). 71289.3 Da, pI = 6.02. Acc. # Q61085. MOUSE. GTP-RHO BINDING PROTEIN 1 (RHOPHILIN).

m/z
submitted MH⁺
matched Delta
ppm start end Peptide Sequence
(Click for Fragment Ions) Modifications
1099.6159 1099.6111 4.2845 418 427 (R)(L)
2211.1389 2211.1331 2.6129 564 584 (R)(G)
2227.2185 2227.1280 40.6326 564 584 (R)SMGEEGVSLQVVSLLPSPEPR(G) 1Met-ox
2272.2089 2272.1515 25.2606 195 214 (R)(A)

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m/z submitted	MH⁺ matched	Delta ppm	start	end	Peptide Sequence (Click for Fragment Ions)	Modifications
1099.6159	1099.6111	4.2845	418	427	(R)(L)
2211.1389	2211.1331	2.6129	564	584	(R)(G)
2227.2185	2227.1280	40.6326	564	584	(R)SMGEEGVSLQVVSLLPSPEPR(G)	1Met-ox
2272.2089	2272.1515	25.2606	195	214	(R)(A)

The matched peptides cover 7% (51/643AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

Percent TIC = 11.8 Mean Error = 18.1977 ppm Data Tolerance = 36.3257 ppm Mean Missed Cleavages = 0.0